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EMAGE:30393

Ccnk cyclin K ( MGI:1276106)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:30393 EMAGE:30393 EMAGE:30393 EMAGE:30393 EMAGE:30393
euxassay_000075_05 euxassay_000075_01 euxassay_000075_02 euxassay_000075_03 euxassay_000075_04
EMAGE:30393 EMAGE:30393 EMAGE:30393 EMAGE:30393 EMAGE:30393
euxassay_000075_06 euxassay_000075_07 euxassay_000075_08 euxassay_000075_09 euxassay_000075_10
EMAGE:30393 EMAGE:30393 EMAGE:30393 EMAGE:30393 EMAGE:30393
euxassay_000075_11 euxassay_000075_12 euxassay_000075_13 euxassay_000075_14 euxassay_000075_15
EMAGE:30393 EMAGE:30393 EMAGE:30393 EMAGE:30393 EMAGE:30393
euxassay_000075_16 euxassay_000075_17 euxassay_000075_18 euxassay_000075_19 euxassay_000075_20
EMAGE:30393 EMAGE:30393 EMAGE:30393 EMAGE:30393
euxassay_000075_21 euxassay_000075_22 euxassay_000075_23 euxassay_000075_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1635
Entity Detected:Ccnk, cyclin K ( MGI:1276106)
Sequence:sense strand is shown

>T1635
TGGCCTCGANGCCAGATTCGGCACGAGGGTCGCTGAGGGTTTTGCCTGAGGCGAGGGGTTGATGTTGGCC
TGAAGAAAGATCTTCAGAGGATTACAACATTCCAGAGTCCCACTGGGGAAGAACAAGATACTTCAATAAA
TGAAGGAGAACAAAGAAAACTCAAGCCCTTCAGTCACGTCAGCAAACCTGGACCACACAAAACCATGTTG
GTACTGGGATAAGAAGGACCTAGCGCACACACCCTCACAACTTGAAGGACTCGATCCAGCCACTGAGGCC
CGGTACCGCCGAGAGGGGGCTCGCTTCATCTTTGATGTGGGCACACGTTTGGGGTTACACTATGACACCC
TGGCAACTGGAATCATTTATTTTCACCGATTTTATATGTTCCATTCCTTCAAACAATTCCCAAGATACGT
GACAGGAGCTTGTTGTCTTTTTCTGGCTGGGAAAGTAGAGGAAACGCCAAAAAAGTGTAAAGATATCATC
AAAACGGCTCGTAGTTTATTAAATGATGTCCAGTTCGGCCAGTTTGGAGATGACCCAAAGGAAGAAGTCA
TGGTGT
Notes:The probe template was PCR amplified from IMAGE:445618 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:445618 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000075 same experiment
 EMAGE:32028 same embryo
 EMAGE:31442 same embryo
 EMAGE:30632 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS