EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30471

Cttn cortactin ( MGI:99695)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_009858_01	euxassay_009858_02	euxassay_009858_03	euxassay_009858_04	euxassay_009858_05

euxassay_009858_06	euxassay_009858_07	euxassay_009858_08	euxassay_009858_09	euxassay_009858_10

euxassay_009858_11	euxassay_009858_12	euxassay_009858_13	euxassay_009858_14	euxassay_009858_15

euxassay_009858_16	euxassay_009858_17	euxassay_009858_18	euxassay_009858_19	euxassay_009858_20

euxassay_009858_21	euxassay_009858_22	euxassay_009858_23	euxassay_009858_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30891
Entity Detected:	Cttn, cortactin ( MGI:99695)
Sequence:	sense strand is shown >T30891 ATGCAGCTCCGTTCAAGGCCGAGCCGAGCTACCGAGGTAGCGAACCTGAGCCTGAGTACAGCATCGAGGC CGCAGGCATTCCTGAGGCTGGCAGCCAGCAAGGCCTGACCTATACATCAGAGCCCGTGTACGAGACTACA GAGGCTCCTGGCCACTATCAAGCAGAGGATGACACCTACGATGGGTATGAGAGTGACCTGGGCATCACAG CCATCGCCCTGTATGACTACCAGGCTGCTGGCGATGATGAGATCTCCTTTGACCCTGATGACATCATCAC CAACATAGAAATGATTGACGATGGCTGGTGGCGTGGGGTGTGCAAGGGCAGATACGGGCTCTTCCCAGCC AACTATGTGGAGCTGCGGCAGTAGGGCTGCCACCCAGAGCCTACCGGCACCAGCACAGGGTTCACACTAC AGAGCATCTGCGTGTGTTTGAGTTGGTTTCTGCTTCCGTTTCTGTTTTTGTTCTTTTTCTGAAGGTGGGG AGGGGAATATTCATATTGACTTTATATTTAATACATCTGCTGATGCTTTGGGGCATGTCCTGCCACAGGA TTTGCTAATGTCTGATGATCATGTTCACAGAGAACATGGGTGATTACTTGCAGCCATCCCAGGTGTTTTA GTTTTGCCAAGCTGACTGTGGTATGTAGCCACTTCAGGGGTTGCGCTTCTCACTAGGTTGGCGGTACATG TGGCCTTGTGCCCACCCCCATCCCCATTGTAGTCAGGTTCCCAGGATGGAGACAGTGCTCCTGGGGTGGC CACTCAGTGAGGAGGCTCTCTGGGGTCCAGTGTTTGGAAGAGGAGGAAAGGGGACC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3981559), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 17790. Forward Primer - name:017790_F_IRAV24-27_I18_Cttn, sequence:ATGCAGCTCCGTTCAAGG; Reverse Primer - name:017790_R_SP6_IRAV24-27_I18_Cttn, sequence:TGGTCCCCTTTCCTCCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009858 same experiment
	EMAGE:30516 same embryo
	EMAGE:30526 same embryo
	EMAGE:30722 same embryo
	EMAGE:30710 same embryo
	EMAGE:32115 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source