EMAGE mouse gene expression spatial database

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EMAGE:30478

Stoml1 ( MGI:1916356)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005498_01	euxassay_005498_02	euxassay_005498_03	euxassay_005498_04	euxassay_005498_05

euxassay_005498_06	euxassay_005498_07	euxassay_005498_08	euxassay_005498_09	euxassay_005498_10

euxassay_005498_11	euxassay_005498_12	euxassay_005498_13	euxassay_005498_14	euxassay_005498_15

euxassay_005498_16	euxassay_005498_17	euxassay_005498_18	euxassay_005498_19	euxassay_005498_20

euxassay_005498_21	euxassay_005498_22	euxassay_005498_23	euxassay_005498_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4731
Entity Detected:	Stoml1, ( MGI:1916356)
Sequence:	sense strand is shown >T4731 CCACGCGTCCGGGGCGTGTCCCGGTTAGAAGTCATGCTTGGAAGGTCTGGCTACCGGGCACTGCCCTTAG GGGATTTTGATCGTTTCCAGCAGTCGAGCTTCGGCTTTCTGGGTTCGCAGAAGGGCTGCTTGTCCCCAGA GCCGGGCAGCGTGGGGCCGGGGGCCGACGCGCCGGAGAGCTGGCCCTCCTGTCTCTGCCATGGCCTCGTC AGTGTCCTGGGGTTCTTGCTGCTGCTGCTCACCTTCCCCATTTCCGGCTGGTTTGCTCTGAAGATTGTGC CCACCTATGAGAGGATGATTGTGTTTCGACTGGGCCGGATCCGTAACCCCCAGGGGCCTGGCATGGTTCT TCTCCTGCCCTTTATTGACTCCTTCCAGAGGGTAGATTTGAGGACCCGAGCCTTCAACGTTCCTCCTTGC AAGCTGGCCTCTAAGGATGGGGCTGTGCTGTCGGTGGGAGCTGATGTCCAGTTCCGCATCTGGGACCCAG TGCTATCCGTGATGGCTGTGAAGGACCTGAACACAGCTACTCGCATGACAGCCCACAACGCCATGACTAA GGCCCTGCTCAGAAGGCCACTGCAGGAGATCCAGATGGAGAAGCTCAAGATCGGTGACCAGCTCCTGCTG GAGATCAATGACGTGACCAGAGCTTGGGGACTGGAGGTCGACCGTGTAGAGCTGGCAGTAGAGGCTGTGT TGCAGCCACCGCAAGACAGCCTGACTGTGCCCAGCCTGGACAGCACCCTCCAACAGCTGGCCCTCCACTT GCTAGGGGGAAGTATGAACTCAGCGGTTGGACGTGTTCCATCCCCAGGGCCAGACACCTTGGAGATGATA AATGAAGTGGAACCTCCTGCCTCTCTTGCTGGGGCCGGGGCTGAGCCCAGCCCGAAGCAGCCCGTGGCTG AGGGGCTCCTCACAGCCCTGCAACCCTTCCTGTCAGAGGCACTGGTCAGCCAGGTTGGGGCCTGCTACCA GTTCAATGTCATCTTGCCCAGTGGCACCCAGAGTATCTACTTCCTGGACCTTACTACAGGACAAGGCCGC GTGGGACATGGAGAGCCTGATGGCATCCCTGACGTGGTGGTGGAGATGGCTGAAGCAGACTTGCAGGCCT TGTTGAGCAAAGAACTTCGGCCCTTGGGGGCCTACATGAGTGGGCGGCTGAAGGTGAAGGGTGACCTGGC CGTGGTCATGAAGCTGGAGGCTGTACTCAAGGCCTTGAAGTAGCCGTGTTGGTTGTCCATCAGACACCTA GCCCTGAACCTGGTGCCAACCCAGAGAAGCCTCTTGGAATAGGAGGCGTTGGTTTGGGCCATGGAAAACT GAGGCCTGAAGATGTTTCAGTCCCAACGAGGAGCCCGGGAGGCTGGGAGACATCTACTGCTGGTCACAGC TGAGTCGGGTGTACCACCCCTTAGCCTGTGGTAGTCCTCTGGACAGGCCTTTGCTCGTTCAATCCCCAAC AAATGCTTAGTGCTGAGTCGGAACACAGAGTGAAGACAGAGAGCCTGGCCTGCCCTACCCAGCTGATAAC CTGACCGACCGGAGAGACGAGAAGCCCAGACGCTTCTGAATGGCAGCATGTGAAGGGTCAAAGCACATGG ATCATGGCTCTGACATTCAGGGAGATCACAGGGAAGGGAAGGTGAGGAAGGGTGGCCCTCAAGAAAGGGT TACTGCAGCTGATCCAGGCGGCCCTGCCAGAACAGGCTGGGTGGGGAGGGCATGTGATAGAACAATTTGC TTTTCTTATGGGTCTGCTCCTGAAGACACCAGGTCCTATCCTCTGGCTCCATCCTGCATGTTTAAGGAGC TGGGCTCAGGGCAGCATGTGGGAGAGCCCTGCTGTTCCGTGCTTATAGAGGCTTGGAGCCTCCAACAAAT AAATGTGGCATTTATGGTGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5037636 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5037636 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005498 same experiment
	EMAGE:30528 same embryo
	EMAGE:31648 same embryo
	EMAGE:31603 same embryo
	EMAGE:31594 same embryo
	EMAGE:29399 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source