EMAGE mouse gene expression spatial database

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EMAGE:30491

Fh1 fumarate hydratase 1 ( MGI:95530)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50427
Entity Detected:	Fh1, fumarate hydratase 1 ( MGI:95530)
Sequence:	sense strand is shown >T50427 CGGTACAGCAGCACCATGTACCGCGCACTCCGTCTCCTCGCGCGCTCGGTCGCCTCCTGCGGGTTCCGTC CGCCGGTGCTGCAGTGTCTGGGGAAGCGACCACCCTCCCGCGGTGTGCTCCGAACGTCGCGCGAATGGCA AGCCAAAATTCCTTCCGTGTAGAGTTCGACACCTTTGGTGAATTGAAGGTTCCAACCGATAAGTATTATG GTGCTCAGACCGTAAGATCTACGATGAACTTTAAGATTGGAGGTGCTACGGAACGTATGCCAATCCCAGT CATTCAAGCTTTCGGCATCTTGAAGCGAGCTGCTGCTGAAGTAAACCAGGAGTATGGTCTCGATCCAAAG ATTGCTAGTGCAATAATGAAGGCCGCAGATGAGGTAGCTGAAGGTAAATTAAATGATCACTTTCCTCTGG TGGTTTGGCAGACTGGATCAGGAACCCAGACAAACATGAATGTAAATGAAGTCATTAGCAACAGAGCAAT TGAAATGTTAGGAGGTGAACTTGGCAGCAAGAAGCCTGTGCACCCCAATGATCATGTTAACAAAAGCCAG AACTCGAATGACACCTTTCCCACAGCAATGCATATTGCTGCTGCAGTGGAAGTTCACAAGGTCCTGTTGC CAGGGTTACAGAAGCTCCATGATGCTCTCAGTGCAAAATCCAAAGAGTTTGCGCAGGTCATCAAAATTGG GCGAACTCACACGCAGGATACTGTCCCTCTTACTCTTGGACAGGAATTCAGTGGTTACGTTCAGCAAGTC CAGTATGCGATGGTGAGAATAAAAGCCGCCATGCCAAGAATCTACGAGCTCGCTGCTGGAGGCACTGCTG TGGGGACGGGGTTAAACACCAGGATCGGTTTCGCAGAAAAGGTGGCCGCAAAAGTAGCAGCACTCACAGG TTTGCCTTTTGTCACTGCCCCGAATAAGTTTGAAGCTCTGGCTGCTCATGATGCTCTGGTTGAGCTTAGT GGAGCCATGAACACTGCCGCCTGCAGTCTAATGAAGATAGCGAATGATATTCGCTTCCTGGGTTCTGGTC CTCGGTCAGGTTTGGGAGAGCTGATCTTGCCTGAAAACGAGCCAGGGAGCAGCATCATGCCAGGAAAGGT GAACCCTACTCAGTGTGAAGCGATGACCATGGTTGCAGCCCAAGTCATGGGGAATCACGTTGCTGTTACC GTTGGAGGCAGCAATGGACATTTTGAACTGAATGTGTTTAAACCAATGATGATTAAGAATGTGCTGCACT CAGCCAGGCTACTGGGAGATGCTTCAGTGTCCTTCACAGACAACTGTGTGGTCGGGATCCAGGCCAACAC AGAGCGGATCAACAAGCTAATGAATGAGTCTTTAATGTTGGTCACAGCTCTTAATCCACATATAGGGTAT GACAAAGCAGCAAAGATTGCCAAGACCGCACACAAGAACGGATCAACCTTAAAGGAAACGGCTATTGAAC TTGGCTATCTCACAGCAGAGCAGTTTGATGAGTGGGTGAAACCCAAGGATATGCTGGGTCCAAAGTGACT TCAATAAATTTATATTAAAATAAACTTAAGAAATTTAC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. .
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_018321 same experiment
	EMAGE:30508 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source