EMAGE mouse gene expression spatial database

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EMAGE:30508

Dlst dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) ( MGI:1926170)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50307
Entity Detected:	Dlst, dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) ( MGI:1926170)
Sequence:	sense strand is shown >T50307 GATCCCGCTGCCTCCGCGATGCTGTCCCGGTCACGCTGCGTGTCCCGGGCGTTCAGCCGCTCGCTTTCTG CCTTCCAGAAGGGGAACTGCCCTCTAGGGAGACGTTCCCTGCCTGGGGTCTCCTTATGTCGGGGACCAGG TTACCCTGACAACAGGAAGATGGTCATTAACAGTGGTAGCGTCTTCAGGGTTCGCTTCTTCCAAACCACG GCTGTGTGCAAGAATGATGTGATAACAGTCCAAACCCCAGCGTTTGCAGAGTCTGTCACAGAGGGAGATG TCAGGTGGGAGAAAGCTGTTGGAGATGCGGTTGCAGAAGATGAAGTGGTGTGTGAGATTGAGACAGACAA GACTTCTGTGCAGGTTCCGTCGCCAGCAAATGGCATCATTGAAGCTCTTTTGGTACCCGATGGGGGCAAA GTTGAAGGAGGAACTCCTCTGTTCACACTCAGGAAAACTGGTGCTGCTCCTGCTAAGGCCAAACCAGCTG AAACTCCTGCTCCAGCCCACAAAGCAGAGCCCGCAGCACCGGCGGCCCCTCCTCCCCCCGCAGCACCTGT GCTCACTCAGATGCCACCAGTGCCCTCACCCTCACAGCCTCCTTCTAGCAAACCAGTGTCTGCAATAAAA CCCACTGCTGCCCCTCCACTGGCTGAGGCAGGAGCTGCTAAAGGTCTGCGTTCAGAACATCGGGAGAAAA TGAACAGGATGCGGCAGCGCATCGCCCAGCGTCTGAAGGAAGCCCAGAACACGTGTGCAATGCTGACGAC TTTCAATGAGGTTGACATGAGTAACATACAAGAGATGAGAGCTCGGCACAAGGATGCTTTCCTGAAAAAA CATAACCTCAAATTAGGCTTCATGTCAGCATTTGTGAAGGCCTCGGCGTTCGCCTTGCAGGAGCAGCCTG TTGTAAATGCAGTGATTGATGATGCAACCAAGGAAGTGGTGTATAGAGATTATATTGACATCAGTGTTGC AGTTGCTACCCCGAGGGGTCTCGTGGTTCCTGTCATCAGGAATGTGGAAACTATGAACTATGCAGATATT GAACGGACCATTAATGAACTAGGAGAGAAGGCCCGGAAGAATGAACTTGCCATTGAAGATATGGATGGTG GTACCTTCACCATCAGCAACGGAGGAGTTTTTGGCTCACTTTTTGGAACACCCATTATCAACCCCCCTCA ATCTGCCATCCTGGGCATGCACGCCATCTTTGACAGGCCTGTGGCTGTGGGAGGCAAGGTGGAAGTTCGA CCTATGATGTATGTAGCCCTGACCTATGACCACCGGCTGATTGATGGCAGAGAGGCTGTGACTTTCCTCC GAAAAATCAAGGCAGCAGTAGAAGATCCAAGAGTCCTCCTCCTAGACCTTTAGGAGGAAGCCACACATCC TACCTATCGATCATACAGGAACTGAAAACCACTCTTCTCCCTGTACCCTCATCAATCCCAGGTTAGCCTG ATGACAGGCAGGCACATACTATTGGCCTCAAGCACGGAAGCCAGGGCACGATGTAACCAGCGTTCACAAG TCTGTTCTTAGTGTTCCCTCGAGGCTCTTTCTCTCTCCCTGAGACTCTTATCTTCTGCTATCTTGGACCT GAGAGAGAGTGAGAGCCTTAATGGATGCTCATTAATGTTCCTGCCTTTATTCCAGTCCTCTACAAAGGCA ATTTTGCTTTTCTCCAGTAACAGTATGCTATAGGGATACCACCAGGGACCATGTGATTTGAGTTTCTATC TTAGGAAAGTGTTCTCCAGAGATGCCTAGGGGATGCTTTGCCTCCATCCCAGCTGTGCACACTCTGACTT GATGCCACCTGTGTGGAGGTATTGAACACAGGCAAAGAGGTGCTGCTTTGCTTCTTAAATGCACCATCAT TCTGACCTGTCAGTGGCTTCAAGGTGCTCTACCTCTTCCAGAAAGCACAGGCTGGGGTATTTGGGGACAG GCAGGATTGTGCAGCATCCCCTGAGCTTACCTGTTATAGT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. .
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_018317 same experiment
	EMAGE:30491 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source