EMAGE mouse gene expression spatial database

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EMAGE:30528

Zfp871 zinc finger protein 871 ( MGI:1921793)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_005485_21	euxassay_005485_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4744
Entity Detected:	Zfp871, zinc finger protein 871 ( MGI:1921793)
Sequence:	sense strand is shown >T4744 AAATGTGCAGATCACAGAATGACTAAGAGCCAAAGTGGTTGAGTTTAGAGTCTGTCCTGAAGTAGGAGGC ATTGAGCTGAGGCGGGTGCAAAGAAGAATCTGAAAGGGGACCAATGATGATGTCCTAGCAGTGTAGACAC AGAGATGTCAAGTATACGCGCACCGAACGTTGAAGTAAGTCAATTTGAAGTTGGCTGAATGTCCTGGGCA GGAGAAACCGCACTGGAGATGGAGTCCAAAGGACTGACTCAGTTCTTCCCTGTGGGAGTCAGTGGCCTTT GAGGATGTGGCTGTGAACTTCTCCCTGGAGGAGTGGGCTATGTTGAATCCATCCCAGAAGAACCTCTACA AAGATGTGATGCAGGAAACCTTGAGGAACCTGGCTTCTATAGGGAGCAGTTTGGAGCACCATATCATTGA AAATATATACCGAAACTCCTGGAAAAAACTAAGTGGTCAAGTGGTGGAGGGACTCTATGACTGTAAAGAT GACCATCAATGTGCAGAAATCTTCGACTTCACTACACACAGTATTGTAAACCAGAACTCCTTGCCAGGAG TGTATATATGTGAAAACAGCGGATGTGCAACGGTAGTCATTGGCCATTTATCTCTAAGTGTACTTCTTAA ACCTGACCCAAAGCATGAATCGTGCGAAGATCAGGAATATGGGAAGGAATTATACAATATGGAATATGGG GCCCCCTCCAGTTCTCTCACATCATTTGAGAACTATGAAAGTACTTACACTGTAGAGAGAATCTATAAAA CTGAGCGTTGTGACGAAGACTATAGATATGGCCAAAATGTTGAAAAGACTTTCACTGAAGAAAAGCCCTT TGAGTGTAAGCAGTGTGGGAAGTGTTTCAGTTCCTCCAGGTATTTTCGAAGGCACGAACGACGTCATCGG GCCAAGAAATGCTATATCTGTAAGCAGTGTGGGAAAGCCTTTGCTTTTCCCACTTACCTACAAACACACG AAAGAATTCACACGGGGGAGAAACCGTACGTGTGCTTGCAGTGTGGGAAAACCTTCGCTCATTCTCGTTC CCTTAAAACACATAAAAGAACTCACTCGGTTAGGGACCTCTTTGTGTGCAATTACTGTGGGAAAACCTTA CGTCATTACAGCTCTCTTCAAATTCATACAAGAATTCACACGGGAGAGAAACCTTATGTGTGTAAACCGT GTGGTAAAGCCTTCATTACTTACCGCTCCTTTCGAATACATGAAAGAATTCACACTGGAGAAACACCTTA TGAATGTAAGCAGTGTGGCAACACCTTCATGCGTTGGATTCAGTTGCGAAAGCATGAAATAATTCACAGT GGGGTGAAGCCCTATGCTTGTAAGCTGTGTGGAAAAAGCTTCACTTGTTCTGGGTCCTTGAGAACACATG AAAGGATTCACACTGGGGAGAGACCCTATGTGTGTGACCAGTGTGGATATAGCTTTACTCGGTTAAATTC GCTTTTAAGGCATAAAATAACCCACACCGGTGAGAAGCCCTACGTGTGTAATCATTGTGGGAAGACTTTC CCTCGATCTGCGTCTCTTCAAAGACATGTCAAAATTCACACCGAGGAGAAACCCTATGTATGCAAACAGT GTGGCGTTGCCTTCCCTAGTTCTGCTGACCTCTTAGAACACGAGCAAACCCATATTGACGAGAAACCGTA CATATGTGAGCAGTGCGGCAACGCCTTCGTGCTGTGGAGTCAGTTTCAGAAGCATAAAGCCCTTCACGCC GTCCCAGTGTCCTACGTGTGTAAGCAGTGTGGGAAGAGCTTCACTTCCTCGAGGTCCCTGAAAACCCACG AGAGAATCCACACGGGTGAGAAGCCTTACGAGTGCAAGCAGTGTGGGAAGACCTTCCTGTGGTCCTACTC CCTACAGAGACATGAAAAGACTCACACGGACGGGAAGGTCCACGTGTGTAAGCAGTGCGGGGAAACTTTC CCTTACGACAGTCATCTCCAGGTGCATGAGAAACTGCACTTTGACGATGAGCCTTTTAATTGTAAGTACT GTGGGAAGCCCTTCTTCACTCTGAACCATTTGCATAGACATGAATTACTTCACACTACCCTGGATTCCAG TGTTTTTTAGTCATGGGAGGATTTTTTTCCCCCGCTTTGTGGTTTTCATAGAAATAAGAGAAATCATACT GGAAAAAGACTGGATTTGTGCTAGCAATGTGGAAAACCTTTTGATTTGTTTCATTGAAACAAACGTGCGA TGTCACAATGAGGAGAAACCTTTTTTTAAAACGAAAATCAATGTACAAATGGTTTTCTGAGCTTAAAGCT CCTTGGAATGCATCAAAGAGAAACGGGCCTGTGACTGCGAGCAGTGTGGTCGAGTCATCTGCAGTCCTGG ATCTTGGAGGAGACAGGAGAAAACTCAAGCTGTAGAGAAACTTTTATGTGTATATGTGGTGTTGTATTAA AAAAAAGCCCTTTCTGCTTCCCCATTCCCTCTGAATATGTGGAAGGAGTCACTGGAGAGAAACCCTTCAT AGCTAGGCAATCTTGGATAGTCTTGAGTTATCACATCCTCTTACAGACATGAAAGAACCTACTGTAGAGA AATCTTAAAAACGGGATTTGTGAACTCTTCATTATCAGCTTCATAGAACTAACAAGAATGTACGTGGGGA GAATTCCTATTAGTGGAAACCTCTATGAACACCATTTGGTTCAATGAACAGACAAGGAAAGAACTTGATC CGGAGTGCTAATCTTCTCAAAAGAGATGTTCAAGGTTTCATTTGGCACAGATGGTAACACATATCGGATG AAAACTTTTGCTGTTGAAAAACATTGGAAAATCTAGAGCAAAGTCCTTTGAAATTTTTTTCATGTTTAAT CTGATAAATGTCAGTATTATGAAGGACTGGACATATTTTATTTTATTATTTTTTTTTTGTAGCATGCTCC AGGAAATGCACACTCTGGTGAGAATGTTATCAGTACAGTATTTTGTATTTATTGAAATGTTTCAGGGGCT GATAGGGATGAGTTAGCCTTTAAGAGCATCTTCTGCTCCTTCCAAAGGACATTGAGTTGGCCTTTAAGAG CATTTGCTTTTTAGCACCACGTCACAGGGCTCACAACTGCATGTGACTCTAGTTACAGGAGACACACTTG TACACATAAAAATAAAGCTTAAATATTTTTCTAGTCTTTTTTTTTCCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5040597 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5040597 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005485 same experiment
	EMAGE:31648 same embryo
	EMAGE:31603 same embryo
	EMAGE:31594 same embryo
	EMAGE:29399 same embryo
	EMAGE:30478 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source