EMAGE mouse gene expression spatial database

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EMAGE:30623

Camta2 ( MGI:2135957)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008596_01	euxassay_008596_02	euxassay_008596_03	euxassay_008596_04	euxassay_008596_05

euxassay_008596_06	euxassay_008596_07	euxassay_008596_08	euxassay_008596_09	euxassay_008596_10

euxassay_008596_11	euxassay_008596_12	euxassay_008596_13	euxassay_008596_14	euxassay_008596_15

euxassay_008596_16	euxassay_008596_17	euxassay_008596_18	euxassay_008596_19	euxassay_008596_20

euxassay_008596_21	euxassay_008596_22	euxassay_008596_23	euxassay_008596_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9171
Entity Detected:	Camta2, ( MGI:2135957)
Sequence:	sense strand is shown >T9171 GTTTGGGGTAGAATGGCCGCTGCCGCCGTCACCCGCGGGACCCCGGGAGCACAGACTCCCCCTCCCCCCG GCCCCTCAGGCCGGGGGTGACCTGGCCCCCTGGAACCTTCACCATGAATACCAAGGACACCACTGAGGTT GCTGAGAATAGCCACCACCTGAAGATCTTCCTCCCCAAGAAATTGCTGGAGTGTCTTCCTCGATGCCCAC TGTTGCCTCCAGAGCGGCTCCGATGGAATACAAATGAGGAGATTGCATCCTACCTGATCACCTTTGAGAA ACACGATGAGTGGCTGTCTTGTGCCCCAAAGACAAGGCCTCAGAATGGCTCCATTATCCTCTACAATCGT AAGAAGGTGAAATACCGGAAGGATGGTTACCTTTGGAAGAAACGGAAGGATGGGAAGACTACCCGAGAGG ACCACATGAAACTCAAGGTCCAGGGCATGGAGCCTGTCTCCTGGCAGTGTCTCTATGGCTGCTACGTTCA CTCTTCCATCGTCCCCACATTCCACCGGCGCTGCTATTGGCTGCTCCAGAACCCCGACATCGTCCTTGTG CACTACCTGAACGTCCCAGCCCTGGAGGATTGTGGAAAGGGCTGTAGCCCCATCTTTTGTTCCATCAGTA GCGACCGTCGAGAATGGCTGAAGTGGTCACGTGAGGAGCTCTTGGGACAGCTGAAGCCCATGTTTCATGG CATCAAGTGGAGCTGTGGGAATGGGGCAGAGGAATTCTCCGTGGAACAGTTGGTGCAACAGATCTTGGAC ACCCACCCAACCAAGCCAGCACCCAGAACTCATGCTTGTCTCTGCAGTGGGGGCCTTGGTTCCGGGAGCC TTACCCACAAATGCAGTAGCACGAAACACCGCATCATCTCTCCCAAAGTGGAGCCCCGAGCTTTAGCCCT GGCTTCTATATCCCACTCCAAGCCCCCCGAACCCCCACCACTGATAGCTCCACTTCCTCCAGAGCTCCCC AAGGCACACACCTCCCCATCTTCCTCCTCTTCTTCCTCCTCCTCCTCAGGATTTGCAGAACCCCTAGAAA TCAGGCCTAGCCCTCCTACCTCTCGAGGGGGTTCATCAAGAGGAGGCACTGCTATCCTCCTCCTAACAGG ACTAGAGCAGCGCGCTGGGGGCCTGACACCCACCAGGCACTTGGCTCCGCAGGCTGAGCCTAGACCTCCT GTGAGCTTGGCCGTGGTTGTAGGTTCTGAGCCTTCTGCCCCACCGGCTCCTCCCAGCCCTGCCTTTGACC CTGATCGTTTTCTCAACAGCCCCCAAAGGGGCCAGACATACGGAGGGGGCCAAGGAGTAAACCCAGACTT CCCCGAGGCAGAGGGCACTCACACTCCCTGTCCTGCCCTGGAACCTGCAGCTGCCCTGGAGCCCCAGGCG GCTGCTCGAGGTCTCGCTCCACAGTTGGGAGCAAACGGGAGAAGAGGAAACAAATTCTTTATCCAAGATG ATGATAGTGGGGAGGAACTCAAGGGTCCGGGAACGGTGCCGCCGGTACCTTCATCCCCTCCTTCATCCCC GTCCTCCCCAACCGCCTTGCCGCCATCAGGCAGGGCAACGAGAGGAGAAGCCTTGTTCGGAGGCTCTGCT GGCAGCAGCAGCGAGCTCGAGCCCTTCAGTCTTTCGTCATTCCCAGACCTCATGGGAGAACTCATCAGTG ATGAAGCTCCAGGTGTCCCTGCCCCCGCCCCCCAGCTCTCTCCTGCGCTAAATGCCATCACAGACTTCTC CCCAGAGTGGTCCTACCCAGAGGGCGGGGTCAAGGTGCTCATCACAGGCCCTTGGACAGAGGCCGCAGAG CATTATTCCTGTGTCTTCGATCACATCGCAGTGCCAGCCTCCCTGGTCCAGCCTGGTGTCTTACGCTGCT ACTGTCCTGCCCATGAGGTAGGGCTGGTGTCTTTGCAGGTGGCGGGGCGAGAGGGCCCCCTCTCTGCCTC TGTGCTCTTTGAGTATCGAGCCCGCCGGTTCCTGTCTCTGCCTAGTACACAGCTCGACTGGTTGTCACTG GATGACAGCCAGTTCCGGATGTCCATCCTGGAGCGGCTGGAGCAGATGGAGAAGCGGATGGCAGAGATTG CGGCAGCTGGGCAGGCTCCTGGTCAGGGCCCAGAGGCTCCTCCAATTCAGGATGAAGGCCAGGGCCCTGG CTTCGAGGCACGGGTGGTGGTCTTGGTAGAGAGCATGATCCCGCGGTCCACTTGGAGGGGTCCTGAACGG CTGATCCATGGAAGTCCCTTCCGGGGCATGAGCCTCCTACATCTGGCTGCTGCACAGGGCTACGCTCGGC TCATCGAGACTCTGAGCCAGTGGAGGAGTGTGGAAACAGGAAGCTTGGACTTAGAGCAAGAGGTTGACCC GCTCAATGTGGACCATTTCTCTTGCACCCCTCTGATGTGGGCCTGTGCTCTGGGACACTTAGAAGCTGCT GTGCTTCTTTTCTGTTGGAACCGACAAGCACTGAGCATTCCGGACTCTCTGGGCCGACTCCCCCTGTCTG TGGCTCATTCTCGTGGTCACGTGCGCCTTGCCCGCTGCCTTGAGGAACTGCAGAGACAGGAGCTTTCAGT TGAGCACCCACTCGCTCTATCCCCACCATCCTCTAGCCCAGACACTGGCCTGAGCAGCGCCTCGTCACCC TCCGAGCTGTCAGATGGCACTTTCTCTGTGACATCAGCCTACTCAAGTGCCCCAGATGGCAGTCCTCCCC CTGCTCCTCCGCTGGCCTCTGACATTTCTATGGAGATGATCCCAGGCCAGCTTTCTTGTGGTGCCCCTGA GACACCCTTACTCCTCATGGACTATGAAGCCACTAACTCCAAAGAACCTGCTCCCTCCCCGTGCGGCCCC CCACTAGCCCAGGACAATGGGGCTGCTCCAGAGGATGCTGACAGCCCACCAGCTGTGGATGTGATCCCGG TGGACATGATCTCACTGGCCAAGCAGATCATCGACGCCACACCAGAGCGGATTAAACGAGAGGACTTCTC AGAGTTCCCTGACGCTGGAGCCTCACCACGGGAGCACACAGGCACCGTGGGGCTCAGTGAGACCATGTCC TGGCTGGCCAGTTACCTGGAGAATGTGGATCATTTCCCCAGCTCAGCTCCTCCCAGTGAGCTGCCCTTTG AACGAGGTCGCCTCGCTATCCCCCCTGCACCTTCCTGGGCAGAGTTCCTCTCTGCATCTACCAGTGGCAA GATGGAAAGTGATTTTGCCCTGCTGACTCTCTCAGATCACGAGCAGCGGGAGCTGTATGAGGCAGCACGA GTCATCCAGACAGCTTTCCGAAAGTACAAGGGTCGGAGGCTGAAGGAGCAGCAGGAGGTAGCCGCAGCGG TGATCCAACGTTGTTATCGGAAGTACAAGCAGCTGACCTGGATTGCACTTAAGTTTGCACTCTATAAGAA AATGACTCAGGCGGCCATCCTGATTCAGAGCAAGTTCCGAAGCTACTATGAACAGAAGCGGTTTCAGCAG AGCCGCCGAGCAGCGGTGCTTATCCAGCAGCACTACCGCTCCTACCGCCGCAGGCCGGGGCCTCCTCACC GGCCCTCAGGCCCCCTTCCTGCTCGAAACAAGGGCACCTTTCTCACCAAGAAGCAAGACCAGGCAGCCCG GAAAATAATGAGGTTCCTGCGGCGCTGCCGACACAGAATGAGGGAACTGAAGCAGAACCAGGAGCTGGAG GGGCTGCCCCAGCCAGGACTGGCCACCTGACCTCTCCAACGCCTTTCTTCAAAACCCTGGGAGCCCCTTC TGGCTGTCTAAACAAGGAGAGGGCTTTCGGGACTAGGGGAGCCCCCTGTCGGCAGCTCTTCACCCCTTCA CCTTTTTGGGAGCCCTTCGTAGAACCCCCGCCCCCAGGCCTTACACGTTTCCTCCCTTCTGGTTGTGCCT CCTCTTTTGGTCTCCGGCTCCAGAAGACCCACGCCCCACATATCTGCATCTTCAGCTGTGACCTCCGGAG CCCTGCCTGCACCTTCTCCATATCCCCGTTCCTGCCTGCACCTGACTCGGACCTTCCTGACTTGCCTTAT TTATTTGTTCGAGAGTCTCTGATGAATGTATCCGCCTCGGTCCCCACCGCTGCCTTCGCTGCGCGCGCCC TCGTGTTTCAGGGGTGACTGCCCCCACCTTGACTCCGCATGTTTGCGTCTGTTTCCTCCTTTCCTGGCCC TGTCTTCCCCATCACCCTGACTCTCTGGTCGCCCACGTAGGGGCCAAAGAGGAGGGGGTGTACATAGGAT CGCGAGGCGGAGTCCCGCTCCCGTGGGGAGGGGTGTGTACATAACTGTAACATACAGAGTATAGTGAAGA ATCTATTTAAGGCGCTGCGCGGCCGGGCGTCTGATTCCCTGGCGCGGCGGGAGCCCCCGGCTGGCTCCAC GAGCACTTTCTACTTGTGCATGGGCTTGGTTTATACGAATTGCCATTAAACGTCTCTGCACCAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827800 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827800 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008596 same experiment
	EMAGE:31856 same embryo
	EMAGE:31317 same embryo
	EMAGE:31891 same embryo
	EMAGE:29471 same embryo
	EMAGE:31893 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source