EMAGE mouse gene expression spatial database

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EMAGE:30632

Zfp326 zinc finger protein 326 ( MGI:1927246)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000178_01	euxassay_000178_02	euxassay_000178_03	euxassay_000178_04	euxassay_000178_05

euxassay_000178_06	euxassay_000178_07	euxassay_000178_08	euxassay_000178_09	euxassay_000178_10

euxassay_000178_11	euxassay_000178_12	euxassay_000178_13	euxassay_000178_14	euxassay_000178_15

euxassay_000178_16	euxassay_000178_17	euxassay_000178_18	euxassay_000178_19	euxassay_000178_20

euxassay_000178_21	euxassay_000178_22	euxassay_000178_23	euxassay_000178_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1573
Entity Detected:	Zfp326, zinc finger protein 326 ( MGI:1927246)
Sequence:	sense strand is shown >T1573 ATCTTACGGAGGGCTGGATCGTGACTATGGCCATGGATCCTATGGGGGTCAGAGATCCATGGATTCCTAC CTAAACCAGTCCTATGGCATGGACAATCACAGTGGTGGTGGTGGGGGTAGCAGGTTTGGACCTTATGAGT CTTACGACTCCAGGTCTTCTCTGGGTGGGCGAGATCTGTACAGATCTGGCTATGGTTTTAATGAACCCGA ACAAACCCGCTTCGGAGGTAGTTATGGTGGTCGATTTGAGAGCTCCTACCGGAATAGCCTTGACTCTTTC GGAGGTAGAAACCAGGGCGGGTCTAGCTGGGAAGCACCTTACTCCCGTTCAAAATTGAGGCCTGGGTTTA TGGAGGACAGAGGAAGAGAGAATTACTCTTCCTACAGCAGTTTTTCTTCACCCCATATGAAGCCTGCACC TGTAGGCTCTCGGGGGAGAGGAACGCCTGCTTATCCTGAAAGTACGTTTGGAAGCAGAAGCTATGATGCT TTTGGAGGACCATCCACAGGCAGAGGCC
Notes:	The probe template was PCR amplified from IMAGE:933790 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:933790 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000178 same experiment
	EMAGE:30393 same embryo
	EMAGE:32028 same embryo
	EMAGE:31442 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source