EMAGE mouse gene expression spatial database

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EMAGE:30656

Lypla3 ( MGI:2178076)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003643_01	euxassay_003643_02	euxassay_003643_03	euxassay_003643_04	euxassay_003643_05

euxassay_003643_06	euxassay_003643_07	euxassay_003643_08	euxassay_003643_09	euxassay_003643_10

euxassay_003643_11	euxassay_003643_12	euxassay_003643_13	euxassay_003643_14	euxassay_003643_15

euxassay_003643_16	euxassay_003643_17	euxassay_003643_18	euxassay_003643_19	euxassay_003643_20

euxassay_003643_21	euxassay_003643_22	euxassay_003643_23	euxassay_003643_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6853
Entity Detected:	Lypla3, ( MGI:2178076)
Sequence:	sense strand is shown >T6853 GGTTAACTAGCTGAGCTGCGGCCCGGGCCTAGGCACGATCCCTAATCTCTAAAGCAGCCCCTTGGACCTG CGACCACAGTCTCACGCCATGGATCGCCATCTCTGCACCTGTCGCGAGACCCAGCTCCGGAGTGGCCTCC TGTTACCTCTGTTTCTACTAATGATGCTGGCAGACCTGACGCTCCCGGCCCAACGTCACCCCCCGGTGGT GCTGGTGCCTGGTGATTTGGGTAACCAGTTGGAAGCAAAGCTGGATAAGCCAAAGGTTGTACACTACCTT TGCTCCAAGAAGACGGACAGCTACTTCACACTCTGGCTGAATCTGGAACTGCTTCTGCCTGTTATCATTG ACTGCTGGATTGACAATATCAGGCTGGTTTACAACAGAACATCTCGGGCCACCCAGTTTCCCGATGGTGT GGACGTGCGTGTCCCTGGCTTTGGGGAAACATTTTCTATGGAATTCCTAGACCCCAGCAAGAGGAATGTG GGTTCCTATTTCTACACTATGGTGGAGAGCCTTGTGGGCTGGGGCTACACACGGGGTGAAGACGTTCGAG GTGCTCCCTATGATTGGCGGCGAGCCCCAAATGAAAACGGGCCCTACTTCTTGGCCCTGCGAGAGATGAT CGAGGAGATGTACCAGATGTATGGGGGCCCCGTGGTGCTGGTCGCCCACAGCATGGGCAACGTGTACATG CTCTACTTTCTGCAGCGGCAGCCACAAGTCTGGAAGGACAAATATATCCATGCCTTCGTCTCACTGGGGG CGCCCTGGGGGGGCGTGGCCAAGACGCTGCGTGTCCTGGCCTCAGGAGACAACAATCGCATTCCCGTCAT TGGGCCACTGAAGATCCGGGAACAGCAGCGATCTGCCGTCTCTACCAGCTGGCTACTGCCATACAACCAC ACTTGGTCACATGAAAAGGTATTTGTATACACACCCACGACTAACTACACGCTCCGGGACTATCACCGGT TCTTCCGGGACATCGGTTTCGAAGATGGCTGGTTCATGCGGCAGGACACAGAAGGGCTGGTTGAAGCCAT GACGCCACCCGGGGTGGAGCTGCACTGCTTGTATGGCACTGGTGTTCCCACGCCAAACTCTTTCTACTAC GAGAGCTTTCCTGATCGGGACCCCAAAATCTGCTTCGGCGATGGTGACGGCACGGTGAACCTGGAGAGCG TCCTGCAGTGCCAAGCCTGGCAGAGCCGCCAAGAGCACAGAGTATCATTGCAGGAGCTGCCGGGAAGCGA GCACATTGAGATGCTAGCCAATGCCACCACCTTGGCTTATCTGAAACGTGTGCTTCTGGAACCTTGATGT CCTCCGCCAGGCAGGGCTGCTGGATGGCTGGACCACGGAGCTTTTCTTGGCCTCTGACTCTTTCACGGTC TACAAGCTTGGCAGAGTTTGTGACCATCATTCAAGTCCCAGGAGGCATCTGCCTCGGAGGAATGCTATTT CTCAACCTTTCTTCTGTAGAATGGCCATGAAGAAGGAAGAAGTGAGTGTGGACATTTGAAGGAGACTGCT GCTGCTGCTGCTGCTGAATTGTGACCTCACGCTGGCTCTGGAGGCTGGCTTGTTTGGCCCCTCACCCCAG TCCCTAACTTGGACCGTGCAATCCTCTTAGCTCTCTGGTCTTTCTACTCTAGCTCACTGGGCCCTAACCT TCCCTTTTGAGGGATGTTAGTTACTGAGCTGATGTCCTGCCATGAAAGATTCCACAGAGATCCCCGGGTG ACCTTCCCACGCACTGGCCTCAGGTTGCCCACTACACTGGCCACAGGTGGCTAGAGCTGCTGGCTTCCCT GTGACCTCGCTGGTGGACATTCTGAGTGTGGGGTCTCCTGGAGGCAGTCGGGTAAGCCCCTGCAGGCAGG CCAGTTGCTCGTATTGTCTCCGTGATCCCTCCAGGCTGGGTTCTTTACAGTCTGCTCCTACCTCTCATTT AGCAGCATCCTAGTTCTGGACTGGACAGCAGAGGACCTCTCAATTCTTGGGGGCCATGTCCTAAGATTCC TGATGTCCCAGCTTGTGACGGAGGTCCCGGTACTGAAGCTGGCTCTGTTGGTTCTGGGACCTGTAGTTTA AGGGGAGGGGATGGGGCTTCTTTGAAGGTTCCTAGAAAGAAAGTGAGCTCAAGGGAACAGCCACGGTTGC TTAGAGCTTCCCATGAGTCACCTTCTTGAGAACTCTACCATCATGGTGCACCCCGCTGTGGGGTATTTCT ACTATCCACTTGGGCTGGCACAGGGTTGAGAGGTGGTAGAGTTCCTGCCCAACACTACCACCAGGCAGCT GAATGGGTTTTGGTTTGCAGGAAAACAGTCCAGAGCTTTGAAAAGGCTCCTAAGAGAGCTAGGAACTTAG AGTCCCAGCTTGGGGGTGGGGGGGGATTGTTTGTTTGCCCCAGGGATGCCACATGGTCTCTGTGTGACAG CAGGGATGGGGAATCTGGGACTGCCTTAGGGACAGTGTACCTGACTATGGGCTTGGGTACAGCTTTTAAG TTGGGGTCTCAAAACCACTTAGAAGCTGGACCACACTGCCTTCCCATATCGTTTCTGATGCTGGACTTCC CTTGTTAAGCACATTCCTATCATCTGTCCCTCACCTGAGTGTTGGGACCCCCCCACTTGGGGCCCTGAGC AAACTGTACCTCGATTTTGGCAATAAAAGTATTCTGGATGTTGTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3964729 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3964729 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003643 same experiment
	EMAGE:30649 same embryo
	EMAGE:32000 same embryo
	EMAGE:30657 same embryo
	EMAGE:32029 same embryo
	EMAGE:30697 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source