EMAGE mouse gene expression spatial database

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EMAGE:30657

Mylip ( MGI:2388271)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_003645_06	euxassay_003645_07	euxassay_003645_08	euxassay_003645_09	euxassay_003645_10

euxassay_003645_11	euxassay_003645_12	euxassay_003645_13	euxassay_003645_14	euxassay_003645_15

euxassay_003645_16	euxassay_003645_17	euxassay_003645_18	euxassay_003645_19	euxassay_003645_20

euxassay_003645_21	euxassay_003645_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6846
Entity Detected:	Mylip, ( MGI:2388271)
Sequence:	sense strand is shown >T6846 GGAGCAATTGCGGTGAGGCGACAGCCCCGGCGCACACCCGAGAAGAAGCGGCGGTGGCGGCGGCCCCAGC CATGCTGTGCTATGTGACGAGGCCGGACGCGGTGCTGATGGAGGTAGAGGTGGAGGCAAAAGCCAACGGC GAGGACTGTCTCAACCAGGTGTGCAGGCGTCTAGGGATCATCGAGGTTGATTATTTTGGGCTGCAGTTCA CGGGGAGCAAAGGTGAGAGCTTATGGCTGAATCTGAGAAACCGGATCTCCCAGCAGATGGATGGGCTGGC ACCTTACCGCCTTAAACTGAGGGTCAAGTTCTTTGTGGAGCCTCATCTCATCTTACAGGAGCAGACAAGG CATATCTTTTTCTTGCACATTAAAGAGTCCCTCTTGGCAGGCCACCTCCAGTGTTCTGCAGAGCAGGCCG TGGAACTCAGTGCCCTCCTGGCTCAGACCAAATTTGGAGACTACAACCAGAACACCGCCCAATACAGCTA TGAGGACCTGTGTGAGAAAGAGCTCTCCAGCTCCACTTTGAACAGCATCGTTGCGAAGCATAAGGAGCTG GAGGGCATCAGCCAGGCCTCTGCCGAGTACCAGGTTCTGCAGATTGTGTCAGCGATGGAGAACTACGGCA TAGAGTGGCATGCTGTGAGGGACAGCGAAGGACAGAAACTCCTCATTGGGGTCGGACCTGAAGGCATCTC GATCTGTAAAGAGGACTTTAGCCCTATTAACAGGATAGCTTATCCTGTGGTGCAGATGGCCACCCAGTCA GGAAAGAATGTCTACTTGACCGTCACCAAGGAGTCCGGCAACAGCATCGTGCTCCTGTTTAAGATGATCA GCACCAGAGCAGCCAGCGGCCTCTACCGAGCCATCACCGAAACACATGCATTCTATAGGTGTGACACAGT CACCAGTGCCGTCATGATGCAGTACAGTCGCGACCTGAAGGGCCACTTGGCGTCTCTGTTTCTGAACGAA AACATTAACCTTGGTAAGAAATACGTCTTCGACATCAAGAGAACATCCAAAGAGGTCTATGACCATGCCA GGAGGGCTCTGTACAACGCCGGCGTTGTGGACCTTGTCTCTCGAAGTGACCAGAGCCCCCCCAGCTCACC CCTGAAGTCCTCAGACAGCAGCATGAGCTGCAGCAGCTGTGAGGGCCTCAGCTGCCAGCAGACCCGGGTG CTGCAGGAGAAGCTGCGCAAGCTGAAGGAAGCCATGCTGTGTATGGCGTGCTGCGAGGAGGAGATCAACT CCACCTTCTGCCCCTGCGGCCACACTGTGTGCTGCGAGAGCTGTGCAGCCCAGCTGCAGTCCTGTCCGGT CTGCAGATCCCGTGTGGAGCATGTCCAGCACGTCTACCTGCCCACCCACACCAGTCTCCTCAATCTGACT GTCATCTGATGCGTCCTGCACTCGATGGACAAGCCATGTCCCCACAAGCTGCAGTATTGTAAACTATAAG AATAATAACTTTGTGAAGAGCTATTTCACTCTCAACACCCATCTGCCATGAGACATTTTCAGAAACAAGG AGGAAAAGAAAACAAGAATGTGACCACACCTCTTCCGTGAGGAGAAGCAACAGGCCCCATGGCCACCAGG AAGAACTCTGGGACACGGACACATTCCTTGAACTTTAGGGTTGGTTTTTTTTTTAATGATCAAGTAAAGG AGTAGATAGAATCGTCTTCGTCAGTCAAGTGGCAACATGGCCCAACCGTGGGCACCTTTTAGGAAATGAC GTCATATGTCTCCTTCACTTTTTCCCCGGGCAGCATTTTGTAAGTGTTTTAAGGATTTCCTTGGTTCTTT TTGTATGGTCATGGAGCGCTGAATATTTTTAATAGGGATTTTTTTTCTTAAAGAAATAGTCCTCATTATA AAAGTCATTTCTGTCTTTATAACTCATTCAAGAACAACTGGAAAAGCTGGCAGATTGAAAAAAAAAAAGC AATCCTGTGACTTCCCAAGGGTTGACAGCAATGTTGTCAGATTGGAAGCAGTCTGGCTGAGAGCCAATAG GTAACTCACCGTGGGTGACTTCCTTCCTAGAGCCCTTCCGTTTCCCCTCATTCCACACCCCATGCCTTTC ACTGATAAAAATGCTACCAGTTTGGTTAAGAGACATACATGGTAGAGTCAAGCACTCCCTGGGCTTTGGA GATTGGAAAGCGAGAGTAGCTTCCTTGAAGGAAAAAGATGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG AGAGAGAGAGAGAGAGAGAGAGAGAGATGAGCCAGAGAGCCACTCAGTATGCCCGAGTGGTTCTTCACTT TCCCAAGCACTCACTCCAGCTGCACCCATGGGTGTCGCCTTGCTTGAAGATCAAACTTTCTACAGCCTTA TAGGTTTCTAGATAGTGTCTCCTTTTTGTGTATGTCTTGTTTCTCGTTGTTCGAGTTTTCCTATGTCAGT GCTTCCATACTCATTGTCCTGCCCCCTCGGTGTCTTCCAGAGGTAGGGCTACTTCTTTATGTTTCCATAT TCTAAGTTTTCACCCCCACTTGGGCATTTTGGATGCTAGTGAGCTAGGGGGTTTTCTAGGGTGTCAGGAA ACCTAGCTGACCTCATCGGGTGCAATACTAGCTAAGTTAAAGCTAGAAGCCTACACTGTCACTTTACTGA GATTTCTGAGTCTACGTTTCATATTGCCTTAATGTAGCAGTAATGTGTTTATGCATTTGTTTCTTTGCAC AGACATTTTGTCAGATATTAAAACTCTACTTTTTTATGGCACATATTAGCATATAAGCCTTTATTCCAAG AGGTATTTATTTTTTCACTTGTAAAAAAAATAATGTTTCCACATTTAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3964381 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3964381 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003645 same experiment
	EMAGE:30649 same embryo
	EMAGE:32000 same embryo
	EMAGE:32029 same embryo
	EMAGE:30656 same embryo
	EMAGE:30697 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source