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EMAGE:30695

Brf1 ( MGI:1919558)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T90
Entity Detected:Brf1, ( MGI:1919558)
Sequence:sense strand is shown

>T90
GAAAGAAGCAAGAATAGCGAAGGAGAAGGAGCTTGGCATCTATAAGGAGCACAAGCCCAAGAAGTCTTGC
AAACGCCGGGAGCCAATCCTGGCCAGCACAGCTGGGGAGGCTATTGAGAAGATGCTGGAACAGAAGAAAA
TCTCCAGCAAGATCAACTACAGTGTGCTGCGGGACCTTAACAGCAAGGGTGGAGGCAGCCCACCCAGGGA
CGACTCACAGCCCCCAGAGCGTGCCAGCACCAAGAAGCTGTCACGGAGGAAAAGAGCAACTACCAGAAAT
AGTGCTGATCCTGGGACAAGCACAGGGAAGAGGTTGAGGCCTCTGCTGTCTGCCCAGCCGGCGAAGAAGG
CAGCCGTGGGAGAGGCCTTACTCTTAAGTTCTCCTGCCTTGGGAGCAGAGCCAATCAAGCCATCAGCAGT
CTTGGTAGAGAGTGGCCCTGTGTCGTACCATCCTGAGGAGGATGCAGATGAGGAGGACGCCGAGGACGAG
GATGGAGAG
Notes:The probe template was PCR amplified from IMAGE:556739 using vector specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:556739 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_016926 same experiment
 EMAGE:30696 same embryo
 EMAGE:30673 same embryo
 EMAGE:30672 same embryo
 EMAGE:30698 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS