EMAGE mouse gene expression spatial database

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EMAGE:30808

1700009P13Rik ( MGI:2443508)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006263_01	euxassay_006263_02	euxassay_006263_03	euxassay_006263_04	euxassay_006263_05

euxassay_006263_06	euxassay_006263_07	euxassay_006263_08	euxassay_006263_09	euxassay_006263_10

euxassay_006263_11	euxassay_006263_12	euxassay_006263_13	euxassay_006263_14	euxassay_006263_15

euxassay_006263_16	euxassay_006263_17	euxassay_006263_18	euxassay_006263_19	euxassay_006263_20

euxassay_006263_21	euxassay_006263_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 16 17 weak expression: see section 10 11 12 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7494
Entity Detected:	1700009P13Rik, ( MGI:2443508)
Sequence:	sense strand is shown >T7494 GGGCGCTTGCCTGGGTTTGTGACCCAGGCAGACGAGCTGGGCTCTTGATGCAGCCCTGGCGGAGGCTTTG AACGCTCTCTTACTTTTCCTAGCCGAGGAAGGGTAACCACAGCCTTTTCTCCCTCCGGTCTTATGAAGCT GGTGTTAATGCAACCAAGAATTTAAGACAATGGAAGAGCGAAAAGCAAAGAGGAAGAGTCCCAAGTCTTT CAGTGCCCACTCTACTCAGGTTGTTAATGCCAAAAAAAAATGCCATCCCATCTAGTAAAAGCACAGGGTT TTCAAATCCCACATCGCAGTCAGCTTCTCAGAGGCCAAAGTTAAAAAGGGTGATGAAAGAAAAGAACAAA CCTCCCGGAGGAGAAGGGAAGGGGGCCCAGTCCACACCCATCCAGCACTCCTTCCTCACCGACGTCTCGG ATGTCCAGGAGATGGAGCGCGGCCTCCTCAGCCTGCTGAACGATTTCCACTCTGGAAAGCTGCAGGCGTT TGGAAATGAATGTTCCATAGAGCAGATGGAGCATGTCCGGGGAATGCAGGAGAAATTAGCTCGCTTGAAT TTGGAGCTCTACGGGGAGTTAGAGGAACTTCCTGAGGATAAGCGGAAAGCAGCCAGTGACGCAAACCTGG ACAGGCTTCTGTCGGACGGGGCTGAAAAGCTGACTCAGCGTCTAAAAGTACTTGCCGCTCTTGCAGAAGA CCCAGATTCTGTTTCCAGCACCCACATGGCGGCCCTCGACCCTCTCTAACTTCAGTTCCGGGGATCTGAA GCCTTCTTCTTGCCTCTGAGGGCACCAGGCACACATGTGCTGCACATGCTTACATTTGGGCAAAGCACAC ATACCCATAAAATAAAAATAAATATCTAAAACCTTCTTAAGGGAGCTAGGGAGATGGCTCAGTTGGTAAA ATTCTTGCTACACCAGTGTGAGGGCCTGAGTTCAGATCCCAGCACCTGTGTGTAAAGTGCCTATGATCTC AGCACTCGAGGAGCAGAGACAAGGAGATCTCTGAGGATTACAGGCTGCCCTTTCTTGCTGATATTGTGAG CTTCAGGTTTGGTGAGAGATCCTGTCTCAAAGAAATGAGGTAGAGAACAGTTAAGGAGGACACCCGACCG TGACTTCTGGCCTCCTTATGTGCAGGCGCACAGCAAATGATGCGCACGCGAACATTTTTTAGAGAACAAA GGAGAAGAAAAATCTCTCCCCAGCGTATTTTAGCTTAATGTTCACTTTCTACACCTTTTCTTTTTGTCTT TCACTTTTTAAAAAAATATATAAGACAACTGTCTGCCCACACTCTAAAAGGCCAGCCTGGGCTATATGAG ACCTCTCTCAAAAAAAACAAGTGAACAGCCCTTCAAGCCTGTGCCTTAAGTGTCATCTCTGCCATGTATT ATTTGGTCTCACAAGTATTCAGTTCATGTTTTAGGCTCTATTTGTCTTAAATATAAGTTTAATTTGTATA CCTTTATAGAGCATTTGGAAAAATAAATTTTAAACCAGGAGGTATAGTGCAAGGCTAGTACTTGGTAACA GCAGGTAGTCTAGTTACTGTACCCACCTACCATCCTTTCTTTTTTTAAACTTCTTTGAGGTATTGGAGAT TAAGCCAAGGGCCTCATGGATGCTGGACAAGCACTCCACCACTAAACTACAGCCCCAGCAAACATCTCTT TACTTTGTGCTTCTTCTAAAATTAAAGGTAGTGTGGTCTGGATATCTGTATCCTTCTAGAACTTGTACTT TCAAGTCCTCACTCTGAGGGTGGTGGCAACAGGCGATGGGTCTGTGGAAGGTAATTAGGTCAGGAGTGGG ATTAGTGTCCTAGGAAAAGAGGCTGAAAAGAGACACCCCTCTGTTTCCATTCTGTGAGGACACAGTACAG GGTGCTGTTCTGTGAGCAGACACCAGCTCCTTTAGCATCTTAATCGTGGACTACTGCTCTCTAGAACTGG GAGAAATGAGTTTCTTTTTTCTGCAAGCCACTAATTTAATGATATTTTATTATAAGAACCCAAATAGACT AAGACAAGGGGTGAACAATTCTACTTTAAAGTGCTAATTGAATGACTTGTAAGATATTTTTGTAATAAAA GTTTTAAAGATTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4237399 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4237399 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006263 same experiment
	EMAGE:31352 same embryo
	EMAGE:31782 same embryo
	EMAGE:30612 same embryo
	EMAGE:30567 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source