EMAGE mouse gene expression spatial database

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EMAGE:30813

Fam49a ( MGI:1261783)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_007357_11	euxassay_007357_12	euxassay_007357_13	euxassay_007357_14	euxassay_007357_15

euxassay_007357_16	euxassay_007357_17	euxassay_007357_18	euxassay_007357_19	euxassay_007357_20

euxassay_007357_21	euxassay_007357_22	euxassay_007357_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

olfactory cortex mantle layer

strong

regional

strong expression: see section 07 08 09 10 14 15 16

thalamus mantle layer

moderate

regional

moderate expression: see section 09 10 14 15 16 weak expression: see section 07 08 11 13 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3263
Entity Detected:	Fam49a, ( MGI:1261783)
Sequence:	sense strand is shown >T3263 TGGCCTCGAGCCAGATTCGGCACGAGGGCAATTCAAATCCCAATGACATTCAACTTCAGGAGAAGGCTTG GAATGCAGTGTGTCCTCTGGTGGTGAGGCTGAAGAGGTTTTACGAATTCTCCATCCGTCTTGAAAAAGCA CTTCAGAGTTTATTGGAATCTCTGACGTGTCCACCCTATACACCAACCCAGCACCTGGAACGGGAGCAAG CCCTCGCAAAGGAATTTGCAGAAATCTTACATTTTACCCTTCGATTCGATGAGCTGAAGATGAGGAACCC CGCTATTCAGAATGACTTCAGTTACTATCGAAGAACAATAAGTCGTAACCGTATCAACAACATGCATCTA GACATTGAGAATGAAGTTAATAATGAGATGGCCAATCGAATGTCCCTCTTCTATGCAGAAGCCACACCGA TGTTGAAGACTCTTAGCAATGCCACAATGCACTTTGTCTCCGAAAATAAAACCCTGCCAATAGAGAATAC CACGGACTGCCTCAGTACGATGACAAGCGTCTGTAAAGTCATGCTGGAGACACCGGAGTACAGGAGCAGG TT
Notes:	The probe template was PCR amplified from IMAGE:2779968 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2779968 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007357 same experiment
	EMAGE:30803 same embryo
	EMAGE:30834 same embryo
	EMAGE:30815 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source