EMAGE mouse gene expression spatial database

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EMAGE:30815

Accn3 ( MGI:2159339)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007353_01	euxassay_007353_02	euxassay_007353_03	euxassay_007353_04	euxassay_007353_05

euxassay_007353_06	euxassay_007353_07	euxassay_007353_08	euxassay_007353_09	euxassay_007353_10

euxassay_007353_11	euxassay_007353_12	euxassay_007353_13	euxassay_007353_14	euxassay_007353_15

euxassay_007353_16	euxassay_007353_17	euxassay_007353_18	euxassay_007353_19	euxassay_007353_20

euxassay_007353_21	euxassay_007353_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

weak

spotted

weak expression: see section 09 10 11 12 13 17 18

facial vii ganglion

weak

spotted

weak expression: see section 04 05

vagus x ganglion

weak

spotted

weak expression: see section 07 17 18

trigeminal v ganglion

weak

spotted

weak expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

pectoral girdle and thoracic body wall muscle

weak

regional

weak expression: see section 03 04 05 06 07 08 20

vestibulocochlear viii ganglion

weak

spotted

weak expression: see section 05 06 07 08 17 18 19 20 21

glossopharyngeal ix ganglion

weak

spotted

weak expression: see section 05 06 07 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3262
Entity Detected:	Accn3, ( MGI:2159339)
Sequence:	sense strand is shown >T3262 TGGCCTCGAGCCAGATTCGGAAGAGGGGGAATGGAGTGTCTGGGGCCTTAAAGGCCCCTCCCTGCAGAAG AGACCCCATTTGAGGTGGGGATCCGAGTGCAGATCCACGGCCAGGAGGAACCCCCTGCCATTGACCAGCT GGGCTTCGGTGCTGCCCCAGGCCACCAGACTTTTGTGTCCTGCCAGCAACAGCAACTGAGTTTCCTGCCA CCACCCTGGGGTGACTGCAATACCGCATCTGTGGATCCCGACTTTGATCCAGAGCCCTCTGATCCCCTGG GTTCCCCTAGCTCCAGCCCTCCTTATAGCTTAATAGGGTGTCGCCTGGCCTGTGAGTCACGCTATGTGGC TCGGAAGTGCGGATGTCGAATGATGCATATGCCTGGAAACTCCCCAGTGTGCAGCCCCCAGCAGTACAAG GACTGTGCCAGCCCAGCTCTGGACGCTATGCTGCGAAAGGACACTTGTGTCTGTCCCAACCCGTGCGCCA CTACACGCTATGCCAAGGAGCTCTCCATGGTGCGGATTCCCAGCCGCGCTTCAGCTCGCTACCTGG
Notes:	The probe template was PCR amplified from IMAGE:2779963 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2779963 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007353 same experiment
	EMAGE:30803 same embryo
	EMAGE:30813 same embryo
	EMAGE:30834 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source