EMAGE mouse gene expression spatial database

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EMAGE:30817

Actl6b ( MGI:1933548)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005177_01	euxassay_005177_02	euxassay_005177_03	euxassay_005177_04	euxassay_005177_05

euxassay_005177_06	euxassay_005177_07	euxassay_005177_08	euxassay_005177_09	euxassay_005177_10

euxassay_005177_11	euxassay_005177_12	euxassay_005177_13	euxassay_005177_14	euxassay_005177_15

euxassay_005177_16	euxassay_005177_17	euxassay_005177_18	euxassay_005177_19	euxassay_005177_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 04 05 06 14 15 16 17 moderate expression: see section 13

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 12

spinal cord

strong

regional

strong expression: see section 07 08 09 10 11 12 13

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 14 15

trigeminal v nerve

strong

regional

strong expression: see section 14

thoracic ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11

retina

strong

regional

strong expression: see section 01 02 03 20

dorsal root ganglion

strong

regional

strong expression: see section 09 10 12 moderate expression: see section 04 05 06 07 08 11 14 weak expression: see section 13

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 06 16 17 18

vagus x ganglion

strong

regional

strong expression: see section 06 14

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 15 16 17 18 19

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 08 09 10 11 13 14 15 16 17

cervical ganglion

moderate

regional

moderate expression: see section 06 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9629
Entity Detected:	Actl6b, ( MGI:1933548)
Sequence:	sense strand is shown >T9629 GGGATCCCGGGAGCTGTCCGGCCGCCTCGGTGCTGATCCCGCCGCCGCCCACGGGCCGCGAGCAGCGTAG AGAGGGCACTATGAGCGGGGGCGTCTACGGCGGAGATGAGGTGGGGGCGCTCGTCTTTGACATTGGCTCT TTCTCAGTCCGAGCTGGGTACGCTGGGGAGGACTGCCCCAAGGCTGACTTCCCCACCACTGTGGGGCTGC TGGCCGCAGAAGAGGGGGGCGGGCTGGAGCTGGAGGGGGAGAAAGAGAAGAAAGGGAAGATTTTCCACAT CGACACCAATGCCCTGCACGTGCCTCGGGATGGAGCGGAGGTCATGTCGCCCCTCAAGAATGGCATGATT GAGGACTGGGAGTGCTTCCGAGCCATCCTGGATCATACCTACAGCAAACATGTCAAGTCCGAGCCAAACC TGCACCCAGTACTCATGTCGGAGGCTCCGTGGAACACTCGGGCCAAGCGGGAGAAGCTGACGGAGCTGAT GTTCGAGCAGTACAACATTCCTGCCTTCTTCTTATGCAAGACGGCCGTGCTCACAGCCTTTGCAAATGGA CGCTCCACAGGCCTGGTGCTGGACAGTGGGGCCACCCACACTACAGCCATCCCAGTCCATGATGGCTATG TCCTACAGCAAGGCATCGTCAAGTCCCCCCTGGCAGGGGACTTCATCTCCATGCAGTGCCGGGAGCTCTT CCAGGAAATGGCTATTGATATCATTCCTCCTTACATGATTGCAGCCAAGGAGCCTGTACGGGAGGGAGCC CCCCCAAACTGGAAGAAGAAGGAGAAGCTACCCCAAGTCTCCAAGTCCTGGCATAACTACATGTGTAACG AGGTGATCCAAGACTTCCAGGCCTCCGTACTGCAGGTGTCTGATTCCCCTTACGATGAACAGGTAGCTGC ACAAATGCCCACTGTGCATTATGAAATGCCCAATGGCTACAACACAGACTACGGCGCTGAGCGACTTCGA ATCCCTGAGGGCCTGTTTGATCCCTCTAATGTCAAGGGCCTGTCCGGGAATACCATGCTAGGAGTGGGTC ACGTGGTCACCACCAGCATCGGCATGTGTGACATTGACATTCGCCCGGGTCTCTATGGCAGTGTCATTGT CACTGGCGGCAACACTCTGCTTCAGGGGTTCACAGACAGACTTAATCGGGAGCTTTCTCAGAAGACCCCA CCGAGCATGCGTCTTAAGCTCATCGCCAGCAACAGCACCATGGAACGCAAGTTCAGCCCCTGGATTGGAG GCTCCATCTTGGCCTCACTGGGCACATTCCAGCAGATGTGGATCTCCAAACAGGAATACGAGGAGGGAGG GAAGCAGTGCGTGGAGCGGAAGTGCCCCTGAAGTTGCCCCCCTCCCCCAAATACCCGTCCACCCCATCCA CGGAGAAACGCCAGAGGGGCGTTCTACCAGCCAGAAATCCAAAAGCGCTGAACTCTCCCTTTCCCATTAC CCTCTTCTCTCTTACCCACATCTTCTTAGACTGTGATGTTCCTGAGTTGAAAGAAGAAAAAAAAAAAAGA ACTTTTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6534039 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6534039 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005177 same experiment
	EMAGE:30828 same embryo
	EMAGE:29595 same embryo
	EMAGE:31620 same embryo
	EMAGE:29356 same embryo
	EMAGE:29587 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source