EMAGE mouse gene expression spatial database

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EMAGE:30820

A130010J15Rik RIKEN cDNA A130010J15 gene ( MGI:2441776)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002912_01	euxassay_002912_02	euxassay_002912_03	euxassay_002912_04	euxassay_002912_05

euxassay_002912_06	euxassay_002912_07	euxassay_002912_08	euxassay_002912_09	euxassay_002912_10

euxassay_002912_11	euxassay_002912_12	euxassay_002912_13	euxassay_002912_14	euxassay_002912_15

euxassay_002912_16	euxassay_002912_17	euxassay_002912_18	euxassay_002912_19	euxassay_002912_20

euxassay_002912_21	euxassay_002912_22	euxassay_002912_23	euxassay_002912_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 17 18 19 20

lower jaw incisor

moderate

regional

moderate expression: see section 17 weak expression: see section 11 12 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 17 weak expression: see section 11 12 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4339
Entity Detected:	A130010J15Rik, RIKEN cDNA A130010J15 gene ( MGI:2441776)
Sequence:	sense strand is shown >T4339 GTGGGAGGGCACTACGCCGCTGGCGGGCAGGCGGAAGGGCTTCCTGCGCCCCCGAACCTGCGGCGGAAAT GCTAGCCGAGTGTCTTGAGGCAGAGCGCTTCCTTTTGTAACTAGTCTACCACCGCGTAGGCACGTGTGGG ATCTTTCTCAAGTGCTGGGAGCTGAACTGTGTGTGGCCCAGGGAAAGGAACTGTGGCTAGCTAAGGAGGC CATATGTCATGTAACTTCCAGATCTTATGTCAACACCAAGCCCTCAGCTGCTGGTGGCAGCTGCTCAGCA GACTCTGGGCATGGGCAAGAGAAAATGCCCACCTCGAGCTACCTGCCTTCACCTGGCAGGAGAGGTGCTG GCTGTGGCCCGCGGGCTGAAGCCAGCTGTACTCTATGATTGCAACTCTGCAGGAGTATTGGCGCTCCAAA GTTATCTGGAGGAACTACAGGGGCTGGGCTTCCTGGAGCCAGGACTTCACATTCTTGAGATTGGAGAGAA TAACTTTATTGTCAGTCCTGAGTATGCCTGTCAGCATTTGGAACAGACACTGCTTGGTACTGTAGCTTTC GTGGACGTTTCCCGCTCCCAACCTCATCCTTCTGTCCGGTCGGTGGACCAGCTCCCGGACTTGAAATCTC TCATAGCTGACGTCATCACACGTTTTCGAGGACTTAAGAAAGATGTGTCTCAGGGAGTCTCCTACACCAG GCTACATTCCTCAGACTGGAATCTCTGCACTGTATTTGGGATACTCCTGGGATATCCAGTCTCTTACACA TTTGACCTGAACCGTGAAGATGACAACTGCTTAACCATGACTCCCCTGCGGGTGTTTACTGCCCGGATTT CGTGGCTGCCTGGTCAACCATCAATTCTGCTGTACTCCTTTAGTGTTCCAGAGAGTTTGTTCCCAGCCCT GAAAAACTTTCTAAGTGCCTGGGAGAAGGAACTCAGAACCCGATTTAGGGCTCAGAATGCCTTTGCTGAC CTCAGCATCTCCTCTGAGGTGGTCACACTTCCTGCTGTGGCCCTCTGAGTTAACTCTGTCCGTTGAGAAG GTATCAATGAATGTCAATTACGATGAATGTTGTGCAGTCAATTGAACACGTGTGAATGGGGAGAGTCGTT GGCAGCAGTAGTATGATTTCAGTCTTTATATTAGGAGTTCCTAGGTCCTTAGCCGAGGTGTCTGTTTATT TTTCATTGAATGAGAGATGGAAAAGGAGCATTGTGGAGACATGAGTGTACCCTGGGATGGTGGCCTTCCC TGGTGGATAGTGCCTTAGAGGGAAGGAGAATGACTAGGTAATCTATGTATGGAACTCTAACTGAAGTAGG GTGCGAGACAATGATGCTTAAGGGTTACACTTGTGGCTTATTTCTCAGGAATACACAGTTCTAAAGAGTA GTCTTGTGAGATAAAGTTTATTTACAACCTGGTGAAAAAAAAATAAAACTAAGAGTCCGGATTCATTTGG AGGGAAACCCAAGTTATAAAGTACCAGAACTCAAGGTTTTCATGGAGTTGTTTGCATCTGAGTTTTACTT CAGATAGATGCCAGAAGTTCTTCAGATGACCAGGTTGCCTTTGTTTGCCAGGAACACTGCCAGTGCTGTA GCAATCACCACCATCAGCACTGGGAGCCATTGACCACACTGCAGCTGTATGGAAGGAGGTGGAGACAGGA GAGAGCAGCTATGAATTAGCTGGAGAAAGTATGGGCAAGAATAGTCCTTTGGCATTTTAACCCAGTAGAA GACCTCACACTGGTGGCAGAAGGGGCTTTTTTTTTTTTTTCTCTTCAGTGTCTCCCTCACTTTTTGTCAT TTCCACTAGATAAAACCAGTTTACTAGTGGCCTAGAGACTCATATCTGACAGGGGACAGATATTTAAATG CTGATCTGAATGAGGTGGAAATTAACTCAGCAGAATAACACTCTTTGTACACGCCTCAATACTTTTGCAG TCCTCCTCTGCCCTTGTCATTATGTTTACAATAAATCAAAAAAGTACTCTTTCATGCTTGAACCAATACA GGCTGAATATCCTTTTTCTGAAATGCTTGGGATTAGGACTCATTCAAATTTTGTCTTTTTTTTTCCCATT AGTATTTGTTTAGTATTTGTATTAGTATTTGTAGTAGTAGTATTAGTATTTGTTTACCAGTTGAGCAGAC GTAATAAAAAGACATCTGAATACCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4527064 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4527064 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002912 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source