EMAGE mouse gene expression spatial database

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EMAGE:30823

2900011O08Rik RIKEN cDNA 2900011O08 gene ( MGI:1914504)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002917_01	euxassay_002917_02	euxassay_002917_03	euxassay_002917_04	euxassay_002917_05

euxassay_002917_06	euxassay_002917_07	euxassay_002917_08	euxassay_002917_09	euxassay_002917_10

euxassay_002917_11	euxassay_002917_12	euxassay_002917_13	euxassay_002917_14	euxassay_002917_15

euxassay_002917_16	euxassay_002917_17	euxassay_002917_18	euxassay_002917_19	euxassay_002917_20

euxassay_002917_21	euxassay_002917_22	euxassay_002917_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 13 18 19 20 moderate expression: see section 17

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 21 22 23

cornea stroma

strong

regional

strong expression: see section 01 02 03 23

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

cervical ganglion

moderate

regional

moderate expression: see section 10 18 19

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 09 18 19 20

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 18

spinal cord

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15 16 17 18

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 09 19 20

thoracic ganglion

moderate

regional

moderate expression: see section 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4322
Entity Detected:	2900011O08Rik, RIKEN cDNA 2900011O08 gene ( MGI:1914504)
Sequence:	sense strand is shown >T4322 TGAAGAGATGGAGCAGCTTCCTCAGCAACGCCGGGCCCATTAAAACACTGCCGATCTGAGCTGCCTTCCT CCCCGCAGCCTTCAGCAGGGATGGAGTGAACCAGACCCCTGGACCTGCCCGCGGGGAACAGCAATGGAGT TAAAGCAATCTCTGTCTGTTCATCTGGAAGCCGAGAAGCCTCTCAGGCGTTATGGGGCGGTGGAGGAGAC AGCATGGAAAGCGGAGGGACTGGGAAGCCAGCTAGACATCATCTCCATGGCTGAGACCAGCATGATGCCA GAGGAGATCGAGCTGGAGATGGCGAAAATCCAGCGTCTCCGTGAAGTCCTGGTCCGTCGAGAGTCAGAAC TCAGGTTCATGATGGATGACATTCAGCTCTGCAAGGACATCATGGACCTGAAGCAGGAGCTGCAGAACTT GGTCGCCATCCCAGAAAAAGAAAAAACCAAGCTGCAGAAGCAGAGAGAGGATGAGCTCATCCAGAAGATC CACAGACTGGTACAGAAGAGAGACTTCCTGGTGGATGATGCCGAGGTGGAACGCTTAAGGGAACAGGAAG AAGACAAGGAAATGGCTGATTTCCTGAGAATCAAGTTAAAACCTCTAGACAAAGTAACCAAGACTTCAGC CAGCTCCCGGGCAGAGAAGAAGGCAGAGCCCCCACCTAGCAAGCCAACAGTGGCCAAGACAGGGCTGGCA CTCATCAAGGATTGCTGTGGGACCACCCAGTGCAACATCATGTAGCCCCTATGTGGGTGCCCCAGAGCTA TGGGGACCCTTCCTCCCACCCTCTTGTCCTCATAGCCCCCATTTCACAGGGGTACAAGAGCTTTCCAAGG CAGAAGGGATTGACAGCAAGCCCATGACTGCTATCAGGGGCTATGGGTATGGCAGGTGGGCCCAGTCTGT ACAGCGTGTAGCAATAGAGAGCCCCTCACTGTCGCATGACCCTCCCCAGAGCATGCCCAACCCAGGAGTC TGTCTTATCATTTATCTAACCACCAAGAAAGCCCCTCCCTTAAAACAATAGACCTTCACTTTTCTCTAGG TGTATCTAACCTACCATGCAAACGAACTGGGCAAGGGCATAGCCCCAGGTGTGTATAGCTGTGAATTTCA ATAATCTAGCACAGTGTTGGTCCCATTCCCCACCTACCCTCCCAGCTTTGCTGTCAGATACAAGTCCCGT TCCCTGTTTGCCCTCTCCCAGGGACTGTAGCTTGGAGGGTTCCACACAGCCCACACACCTCTTAGGCTCC ATTGTAATCACTTCCCTAAAGACACTGGCCTGTCTATGAAAGCTGAGTGTGTGTGGTGTTGGGTTTACAC CTCCTGCTCCATCCCAGCCTGGTGTGTATCTATCTGCCAGACTCCAGGCTCTTCCTTGATTTGCCATGCC CAGCCCACAGTGGAACAGAGTAGGGAACTGTGACCAAAAGCAGATCCAGTGCTTGGACCAGGTAGCAGAG AACTGCAGGGACCAATATCAAGACCAGAGTGAATGAGAAGGGGACCCAGAAAAGATGCCAACCCACATCC TCTTCACCCAGGGCTGCTGCAAATGAGTACATACAAGTGGGAAGGTCACACTGCTGGCCCAACACACACT GACAGGAAGCCTGACCCAACTATGTCCCATTGTCTGGTGGCGTGTTTGAACCAGCCCCACAATTCGTCTT GTGTAACTTGATGTACATTTTAAATGGCCAGCCCAGCACAGCCTGTGTGAACTCTGTGCGTGTATGTGTG TTATGACTGGCCTAAGTACTTAGCACCAGTCAAGATTTGCTGATGTGCAAACTATCCATCAGAGAAAAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4511454 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4511454 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002917 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source