EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30824

Zcchc18 zinc finger, CCHC domain containing 18 ( MGI:1914245)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002916_01	euxassay_002916_02	euxassay_002916_03	euxassay_002916_04	euxassay_002916_05

euxassay_002916_06	euxassay_002916_07	euxassay_002916_08	euxassay_002916_09	euxassay_002916_10

euxassay_002916_11	euxassay_002916_12	euxassay_002916_13	euxassay_002916_14	euxassay_002916_15

euxassay_002916_16	euxassay_002916_17	euxassay_002916_18	euxassay_002916_19	euxassay_002916_20

euxassay_002916_21	euxassay_002916_22	euxassay_002916_23	euxassay_002916_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 19 20

stomach

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12

kidney calyx

weak

regional

weak expression: see section 09 10 11 12 19 20 21 22

cervico-thoracic ganglion

strong

regional

strong expression: see section 11 12 17 18

spinal cord

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 09 19 20

thoracic ganglion

strong

regional

strong expression: see section 14 15 16

midgut

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 13 18 19 20 moderate expression: see section 08

pancreas

moderate

regional

moderate expression: see section 11 12

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 20 21 22 23

cornea stroma

moderate

regional

moderate expression: see section 02 03 04 24

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23 moderate expression: see section 24

adrenal gland

weak

regional

weak expression: see section 10 11 19 20

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 12 13 16 moderate expression: see section 09 10 11 17 18 19 20

hindgut

moderate

regional

moderate expression: see section 14 15 16 17

cervical ganglion

strong

regional

strong expression: see section 10 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4283
Entity Detected:	Zcchc18, zinc finger, CCHC domain containing 18 ( MGI:1914245)
Sequence:	sense strand is shown >T4283 GCCTAACCCAGACAGCTCTGAGGCTGAGGCGCCATCCTTTCCTCTGCAAGGCCTACGAGGTGAGACAGCT CTTCGCAGCAGACCCTGAGCATCCTCTGCTCATCTACGAAGCAGCTCATCAAAGCCGATTTCAAACGTGA ATTCTGTGTGACTGATTGCAGAGCTGTTTAGACTAGAGGCCCAGGGCACCTGGATCGGAGAGCCTGCGGT CAGAGATCAATCCTACCCGGACAAGAGCTATTGCGGAAAGCACCTGTTGCAGAGAGTAGGCTTGAAATAA AGGTGGAAGCCAGTTTCTGAGCTCTGTTTCTACCCAAACACAAGGAAGAGACTGCTGGGGAACGTTCCTT TGGAGGTTTCTGCAGATTGGACTGCTTTTGTTTGCAGAAAGTCCAGGCGTCTTGTATTCAGATATCCAAT TGTTGTCAGGCATGGCTAGCCTCCTTGCTAGGATGGGTAACAGTCGCCGGCAGAATGCAGCTTTTATGCC TTTTGCCCATTCCATGCTGAGGGCCCTGGGGAGGAGCCTTGGTCCTTTGATTGCCAACATTGCAGAGAGA AACATACAGTCGTTCTCTGGGAGGGCAGAGCTAGGACCCGGGGAAGAAACCTTTGAAAACTGGCTAAGCC AAGTCCATGAGGTCCTGCCAGATTGGCCTATGTCTGAAGAAGAAAAGATCAAGCGCCTGATGAGAACCCT TAGGGGCCCTGCCCGGGAGGCCATGCGTTTGTTCCAGGCTGACAATCCCAATCTAAATGTCGCAGAATTT TTGCGGGCAATGAAGCTGCTCTTTGGAGCGTCTGAGAGCAGTATAACAGCGCATGGCAAATTTTTAAGCA CCCTGCAGGCACAGGGAGAGAAACCATCCCTGTATGTGATCCGTTTAGAGGTGCAGCTACAGAATGCTAT CCAGGCAGGGGTCCTTCCTCAGAGCGAGGCAAACAGGACTCGCCTGCACCAGCTCCTTGTAGGGGCTGAG CTGAGTAGGGAGCTGCGCACCAAGCTTAAGGGGCTTCTCCAAATGCATGCGCATAATGAGCAGGAAAACC TTCCTGACTTCCTGGAGTTGATCAGGATGATAAGGGAGGAAGAGGATTGGGATGAGACATTCCTTAGAAA TAAGCGGCCCAGAAGATCTGAGACAGTGATGGAGCGGGCAGCCAGCCCTGTGGTCTTTCAAGGCTCCCTG CCTATAGTGATAGGCAGTGCCGACTGTAATGTGATAGAGATAGATGATTCCCAGGATGATTCAGATGAAG ATGTGATCCTGGTGGAGCCCGAGGACCCTCCACTCTCATCCCCAGGTGCCTCATCCCTCCGAGGCACAGC CAGTACTCAGGAAGAGATGCTGATTATTGAGTCCCCTGATGATTTTGATGAGGAGTCTCCTTCCACTAGC AGTGGTTCTGGGCAAAGGAATAACGGGCCTGGGGATCTAGGTAGAACCAGAAAGCGCAAGTACCCAATCC GCTGTCCTCATTGTGGTGAAGAGGGCCACGCCAAAGAAACCTGTGACAATACCAGCAACAAGGGCCAGGT TTTTGAGAATTTGATAGTCACTCTGCAGGAGCTGACTCACATGGAGAGACCCAAACCCTCTGTACCATAC TAAGGTGTGACCCTCGGCCCCAGATCAGTGTTTGAATCTCCTCAGAAGCTAATTCTGGAGGAAAAAGGGA TGGGGTGGGCTGCTGACATTAAGGAATTCATTTAAAACAAGCTTTTTCTTTGGAGAAGAAAAAGCAAACT GGGGTACCATGCAACAGAGAGGCATGGGCCCACATCATTCCTTGCTCATTCCATTAACTGCTTAATGGTT GTTTCTCTGTGTGTCTTTCTCTGGTGATTTTAATCTATCCGTGAAGTGGTACCTTTTTGAACCTTCCAAA TGAAATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4505033 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4505033 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002916 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source