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EMAGE:30825

Tbc1d10a ( MGI:2144164)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:30825 EMAGE:30825 EMAGE:30825 EMAGE:30825 EMAGE:30825
euxassay_000737_01 euxassay_000737_02 euxassay_000737_03 euxassay_000737_04 euxassay_000737_05
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euxassay_000737_06 euxassay_000737_07 euxassay_000737_08 euxassay_000737_09 euxassay_000737_10
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euxassay_000737_11 euxassay_000737_12 euxassay_000737_13 euxassay_000737_14 euxassay_000737_15
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euxassay_000737_16 euxassay_000737_17 euxassay_000737_18 euxassay_000737_19 euxassay_000737_20
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euxassay_000737_21 euxassay_000737_22 euxassay_000737_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
vibrissa
weak weak
regionalweak expression: see section 05 06 20 21 22
kidney calyx
weak weak
regionalweak expression: see section 07 08 09 10 16 17 18 19 20
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T944
Entity Detected:Tbc1d10a, ( MGI:2144164)
Sequence:sense strand is shown

>T944
TCTCAGNCTGTTGGCCTACTGGGTCGGCGGAGGCCGGGCGGCTCAGGCGGCTGAGGTGGCCGCTGGCGCT
CTGGTGGCGGGCGCGGCCGATATGGCGAAAAGCAGCAGAGAGAATGGGCCGCGCGAGCCCGCAGCAGGCG
GGAGCCTGTCTGGGACCCGGGAAAGCCTGGCCCAGGGCCCCGACGCCGCCACCGCCGACGAGCTGAGCTC
GCTCGGCTCCGACTCGGAGGCCAACGGCTTCGCCGAGCGCCGCATCGACAAGTTCGGATTCATCGTGGGT
TCGCAGGGCGCCGAGGGCGCACTGGAGGAAGTTCCCCTGGAGGTGCTGAGGCAGAGGGAGTCCAAGTGGC
TGGACATGCTCAACAACTGGGACAAATGGATGGCCAAGAAGCACAAAAAGATTCGTCTTCGATGCCAGAA
GGGCATCCCACCTTCTCTGCGCGGACGGGCTTGGCAGTACCTGTCGGGAGGCAAGGTGAAACTTCAGCAG
AACCCTGGGAAGTTTGATGAGCTGGACATGTCTCCTGGGGACCCCAAGTGGCTA
Notes:The probe template was PCR amplified from IMAGE:1970058 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1970058 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000737 same experiment
 EMAGE:31804 same embryo
 EMAGE:29161 same embryo
 EMAGE:29183 same embryo
 EMAGE:29352 same embryo
 EMAGE:31799 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS