EMAGE mouse gene expression spatial database

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EMAGE:30826

Add3 adducin 3 (gamma) ( MGI:1351615)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_002919_06	euxassay_002919_07	euxassay_002919_08	euxassay_002919_09	euxassay_002919_10

euxassay_002919_11	euxassay_002919_12	euxassay_002919_13	euxassay_002919_14	euxassay_002919_15

euxassay_002919_16	euxassay_002919_17	euxassay_002919_18	euxassay_002919_19	euxassay_002919_20

euxassay_002919_21	euxassay_002919_22	euxassay_002919_23	euxassay_002919_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 moderate expression: see section 02 03 04 05 06 07

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 11 12 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4296
Entity Detected:	Add3, adducin 3 (gamma) ( MGI:1351615)
Sequence:	sense strand is shown >T4296 CCACGCGTCCGGGGATGAGCTACATAAACATGTGGTGAGTTGGTGAAAACAGAAGACTAAAATAGGGCAC AGTGGCTGGAAAAGAAAGAATCCAGAGAGGAAGACGACTGGAGACATGAGTTCCGACACCAGCCCAGCTG TGGTTACCACGCCTCCTCCTCCCAGCATGCCTCACAAAGAGAGGTATTTTGACCGAATCAATGAAAGTGA TCCGGAATACCTTCGAGAGAGGAACATGTCTCCTGATCTACGACAGGACTTCAATATGATGGAGCAGAGG AAGCGAGTCACTCAGATCCTGCAGAGCCCTGCCTTTCGGGAAGACCTGGAATGCCTCATCCAAGAGCAGA TGAAGAAAGGCCACAACCCAAGCGGCTTATTAGCGTTACAGCAGATTGCGGATTACATCGTGACCAGCTC CTTCTCGGGCTTCTCCTCGCCCTCCCTGAGTCTTGGCATGGTCACACCTATTAATGACCTTCCTGGTGCA GATACATCCTCGTATGTGAAGGGAGAAAAGCTTACTCGCTGCAAACTTGCCAGCCTCTACAGGCTTGCAG ACTTGTTTGGGTGGGCGCACCTGGCAAATACGTACATCTCGGTACGAATAAGTAAGGAGCAAGACCACAT TATAATAATTCCCAGAGGCCTCTCTTTTTCTGAAGCGACAGCCTCCACTTTGGTGAAAGTCAACATAATC GGAGAAGTGGTGGACCAGGGGAGCACTGATCTGAAAATCGACCACACGGGCTTCAGCCCGCATGCTGCCA TCTATTCAACACGCCCCGATGTGAAGTGTGTGATCCACATCCACACCCTTGCCACAGCAGCGGTGTCCTC CATGAAGTGTGGGATCCTTCCAATTTCCCAAGAGTCTCTCATCCTGGGAGATGTCGCCTACTATGACTAC CAGGGGTCGCTTGATGAGGAGGAAGAAAGAATTGAACTTCAGAAAGTGCTGGGTCCAAGCTGTAAGGTGC TCGTCCTCAGGAACCATGGCATGGTGGCTCTTGGCGAGACCCTCGAGGAGGCTTTCCATTACATCTTTAA TGTACAGATGGCCTGTGAGATCCAGGTGCAGGCAGTAGCAGGGGCAGGCGGAGTAGACAATCTGCTCGTA CTGGATCTTCAGAAATATAAAGCTTTCACCCACGGTGTCGCCATGTCTGGAGGAGGCGGTGTAAACATGG CTTCCCATCAGAAGTGGAAGGTTGGCGAGATTGAGTTTGAAGGGCTGATGAGGACCCTGGACAACTTGGG CTACAGAACAGGCTATGCGTACAGGCATCCTCTGGTCCGAGAGAAGCCTAGGCACAAGAGTGACGTGGAG ATCCCTGCAACCGTGACTGCCTTCTCCTTTGAAGATGACAGTGCTCCACTCTCTCCCCTCAAGTTCATGG CACAGAGACAGCAGCGTGAAAAAACAAGATGGCTGAATTCACCAAATACTTACATGAAGGTGAATGTGCC GGAGGAGTCTCGGAACGGGGAAACTAGTCCCAGGACCAAAATCACGTGGATGAAAGCAGAAGACTCCTCT AAAGTCAGTAGTGGGACACCGATCAAAATTGAAGACCCCAATCAGTTTGTTCCTTTAAACACAAACCCGA CGGAGGTGCTAGAAAAGAGAAATAAGATCCGGGAACAGAATCGCTATGATCTGAAGACAGCAGGGCCGCA GTCTCAGCTGCTGGCGGGGATCGTTGTGGACAAGCCGCCTTCGACCATGCAGTTTGACGATGACGATCAG GGCCCACCAGCTCCTCCTAACCCATTCAGCCACCTCCTGGAAGGAGAACTGGAAGAGTATACGAAGACAA TCGAGAGAAAGCAGCAAGGCCTGGACGACGCTGAGCAGGGGTCACTCTCAGACGACGCTGCATCTGTTTC ACAAATTCAGTCTCAAACTCAGTCACCGCAGAGTGTCCCTGAGAGATTAGAAGAAAACCACGAGCTGTTT TCCAAGAGCTTCACCTCCATGGACGCGCCTGTCATGATCATGAACGGCAAGGACGAGATGCATGATGTCG AAGACGAGCTTGCTCAGCGAGTCAGTAGGCTGACCACAAGCACGACCATAGAGAACATCGAGATCACCAT TAAGTCCCCAGAAAGAACTGAAGAGGTCCTGTCGCCTGATGGCTCACCTTCAAAGTCGCCCTCCAAGAAA AAGAAGAAATTCCGCACGCCTTCTTTTCTCAAAAAGAACAAAAAAAAGGAGAAAGTTGAGGCCTAAGTAG TCCTTTTGTAATTTGTATATTACATTGTGGCATTGCACACTTAAATACCATATTTTAGTTAATCGTTAAT ATACTGTGGTAGATCAGATTGGGAGTATGTAGCAAACTGGACTTTAAAAAACTGGAAAGAGGTTTTACTA AAAGAAAGATATTATTGAAAACTTTCAAATTCATCTCTTTTTATATGTCCGAAAACGGCCGAGCTTTTCA GCAGTGGCTGGCTTGGCCGACTCTGCCGTCCCTGGCAGAGCGTTGTTACTGTTGCTCCTTAGCAGCTGCT GCCTGGTGCCTAGGCCTCTGCAGCACATGTGCTGGGAAGGAGCTGCCAGTGAGCACGAGACAATGCTTAC TTGTCACTAATCAGACCTTGAGTTTATCATCGTGGTTATTGACTGTATCTTTGGGTCCCCACTGTTTCCA TGGGCATTAATGGGATGTTTTAAAAGCTTCTACAAGACTCTAGAGCATTAGTATACATTGGCACATCATT TTTTAAAAAATGTTTTAAGAAAAGATTTGTCTGGGTTTTTACTCATAGTATGAAATTCCCCTACCTGAAG TAACTGTTTGCCAAAAAGTCATTTTAATAAACTATAATTTTTGAAGAACTTCCTTTTTTATTAGTTTAGA AAACCCCTTATTTTTCATAGAGGGGATTTTGTACACATAATATGAGTTATTTAGTTTAACTCTTGCAAAA AAACAATTTTTGTATGTTAAAACGTTTGTATACCTTCAGTATAAGGTGTCATGTGCATGTTAGCCAATCA GTCAGGGGTAAAGCCTGCCCGAGTGCCATCCAGGAGTCCAGGGGCGTGGCACCCAAGGGCATATCAAGGC CTCGAGTCGTATTTGAACCATATAATGTATTTTTCTCTTAGTATTGCTGATGGATGAAGAGGCTGAGGGG AAGGGAAGAGTAAGAGACAGGACAGGACTGAGTCCTGTGTCCACAGAAGAAAGAACATGGAGATCGACTT GCTGAAAATAAGGCCCAAGTTTCTAAAATCCTGTTTCCTGCCAAAATCAATGTCTTGGTTGACATTTGAA TTTTAAAAGAGTTAAAGTGTACATCCTTTTAGAAGACGGCACTAGAATAAATCAGCAGGTCTAATCCCAC CAGCGAGAAAACTACCACGTCTTGATTTTTGCAAACTTAATCTTGATTTTTATAAACTTAAATTTTCTTT TAACTTCCTTTCTACTGTAAATACAAATTTAAGCCTAGATTTAATATAGTTTTCTCCATTTCCCCAAGTA ATGTCGCAGATCAATGTAAATGGATGACCCCAATACTCCACGAGTGGAAGTAAAGCGCCCTGCGGGTGAC TTGGCACGCAAACGTGACGTGACGTTGGCCGCAGTGCTCTCTGGGCTGGCTCTGTCTGACCCTGTGATTG ATGCTGGTCCTCTTAGACCTCACTAACTACTGGAGCCTGTTAATCTCGTAGTGGAAACTTTGAGTTGCTG AATTGTGCATCTGAGGATTGTTTTATGTCCTACATCATCTGTGTTGTGCTACAACGTGTGGAGACACAGT TACCTGTGAACTCTCCTTTTCCATAACTTTTAAAGAATATATATATCTAAATGAATGCAATGTGCATAAA TATTTTTTAAACACAACAGTAAACTATTGCACCTTTCGCTAACGCTTCTCTTTACTTGCTTTGACATAAT GAATGAGCCAGTGAACTCAGTGTCCTGTGGAAAATGTATAAAAGTTATCTGATATTGCTCTTAGATGTTA TGCTAATTAATGTTAAATCACAATAAACGGTATTTTAAATATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4506748 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4506748 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002919 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source