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EMAGE:30834

Nnat neuronatin ( MGI:104716)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 04 05 06 07 15 16 17 18
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 07 08 09 10 14 15
spinal cord
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 16 17
trigeminal v nerve
strong strong
regionalstrong expression: see section 06 07 14 15
thoracic ganglion
strong strong
regionalstrong expression: see section 11 12 13 14
left lung
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10
esophagus
strong strong
regionalstrong expression: see section 09 10 11
diaphragm
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
facial vii ganglion
strong strong
regionalstrong expression: see section 03 04 05 16 17 18 19
eye skeletal muscle
strong strong
regionalstrong expression: see section 02 03 04 05 18 19 20 21
vagus x ganglion
strong strong
regionalstrong expression: see section 05 06 15 16
peritoneal component
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
vertebral axis musculature
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
trigeminal v ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 07 14 15 16 17 18 19 20 21
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 12 13 14 15 16 17
cervical ganglion
strong strong
regionalstrong expression: see section 06 07 08 14 15
neural retina
strong strong
regionalstrong expression: see section 01 02 21 22 23
mesothelium
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
pericardial cavity
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
right lung
strong strong
regionalstrong expression: see section 11 12 13 14 15 16 17 18 19 20 21 22
mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
stomach
strong strong
spottedstrong expression: see section 01 02 03 04 05 06 07
tongue
strong strong
spottedstrong expression: see section 09 11
brain
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 04 05 17 18
tail paraxial mesenchyme
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13
tongue muscle
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 moderate expression: see section 06 13
midgut
strong strong
spottedstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
dorsal root ganglion
strong strong
regionalstrong expression: see section 07 08 09 10 11 15 16 17
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3265
Entity Detected:Nnat, neuronatin ( MGI:104716)
Sequence:sense strand is shown

>T3265
TGGCCTCGAGCCAGATTCGGACGAGGTCGACCACCCACCCACTTTCGGAACCATGGCCGCAGTGGCAGCA
GCCTCGGCAGAACTGCTCATCATCGGCTGGTACATCTTCCGCGTGCTGCTGCAGGTGTTCCTGGAATGCT
GCATTTACTGGGTAGGATTCGCTTTTCGAAATCCTCCAGGGACACAGCCCATTGCGAGAAGTGAGGTGTT
CAGGTACTCCCTGCAGAAGCTGGCGCACACGGTGTCCCGGACCGGGCGGCAGGTGCTGGGGGAGCGCAGG
CAGCGAGCCCCCAACTGAGGCCCCAGCTCCCAGCCCTGGGCGGCCGTGTCATCAGGTGCTCCTGTGCTTC
TCGACCAGCATGGGAGCCAGTGCCGCGCAGGAATGGGGGGTCCCCTGTGTTCCCTCGTCAGAGGAGCACT
TGCCAAGGTCAGTGAGGGGCCGGTAGGCCCCCAGAAAAGCAGCACCGACAATGATGAAGATATCAGTTCC
CTTCCCAGCCCCTTTGCCCCTGTCCCACTACCGGCGGGTGGGAGAGGAGGGGGGAAGAGGGG
Notes:The probe template was PCR amplified from IMAGE:2779989 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2779989 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_007364 same experiment
 EMAGE:30803 same embryo
 EMAGE:30813 same embryo
 EMAGE:30815 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS