EMAGE mouse gene expression spatial database

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EMAGE:30837

Stmn3 stathmin-like 3 ( MGI:1277137)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000708_13	euxassay_000708_01	euxassay_000708_02	euxassay_000708_03	euxassay_000708_04

euxassay_000708_05	euxassay_000708_06	euxassay_000708_07	euxassay_000708_08	euxassay_000708_09

euxassay_000708_10	euxassay_000708_11	euxassay_000708_12	euxassay_000708_14	euxassay_000708_15

euxassay_000708_16	euxassay_000708_17	euxassay_000708_18	euxassay_000708_19	euxassay_000708_20

euxassay_000708_21	euxassay_000708_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 15 16 17 18

facial vii ganglion

strong

homogeneous

strong expression: see section 04 05 06 18 19 20 21

vagus x ganglion

strong

homogeneous

strong expression: see section 08 17 moderate expression: see section 16 weak expression: see section 09

trigeminal v ganglion

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 17 18 19 20 21 22

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 10 11 14 15 16

hindgut

moderate

regional

moderate expression: see section 11 12 13 14 15

vestibulocochlear viii ganglion vestibular component

strong

homogeneous

strong expression: see section 04 05 06 07 17 18 19 20

superior glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 06 07 18

vestibulocochlear viii ganglion cochlear component

strong

homogeneous

strong expression: see section 06 18

inferior glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 06 07 18

retina

moderate

regional

moderate expression: see section 01 02 03 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3778
Entity Detected:	Stmn3, stathmin-like 3 ( MGI:1277137)
Sequence:	sense strand is shown >T3778 TGGCCTCGAGCCAGATTCGGCACGAGGATTTGCTCCTGCTTCTACTCTCAGCCTCACCCGAACACCATCT ACCAGTATGGAGATATGGAAGTGAAGCAGCTGGATAAGCGGGCCTCTGGCCAGAGCTTCGAGGTCATCCT CAAGTCTCCTTCTGACCTATCTCCAGAGAGCCCTGTGCTCTCTTCTCCTCCCAAGAGGAAGGATGCTTCC TTGGAAGAGCTGCAGAAGCGGCTGGAGGCAGCTGAGGAGCGGAGGAAGACTCAGGAGGCTCAGGTGCTGA AGCAGCTGGCAGAGCGGCGTGAGCATGAACGGGAGGTGCTGCACAAGGCGCTTGAAGAAAACAATAACTT TAGCCGCCTGGCGGAGGAGAAGCTCAACTACAAGATGGAGCTGAGCAAGGAGATCCGCGAGGCGCACTTG GCAGCGCTGCGCGAGCGGCTGCGCGAGAAGGAGCTGCACGCTGCTGAGGTGCGCAGGAACAAGGAGCAGC GGGAGGAAATGTCTGGCTAAGGAGTATTGGACCCAGCGGCGACAAGA
Notes:	The probe template was PCR amplified from IMAGE:388906 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:388906 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000708 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source