EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30841

Dnajb13 DnaJ (Hsp40) related, subfamily B, member 13 ( MGI:1916637)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006246_01	euxassay_006246_02	euxassay_006246_03	euxassay_006246_04	euxassay_006246_05

euxassay_006246_06	euxassay_006246_07	euxassay_006246_08	euxassay_006246_09	euxassay_006246_10

euxassay_006246_11	euxassay_006246_12	euxassay_006246_13	euxassay_006246_14	euxassay_006246_15

euxassay_006246_16	euxassay_006246_17	euxassay_006246_18	euxassay_006246_19	euxassay_006246_20

euxassay_006246_21	euxassay_006246_22	euxassay_006246_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

midbrain ventricular layer

moderate

regional

moderate expression: see section 11 12

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 12

lateral ventricle choroid plexus

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 11

pons ventricular layer

moderate

regional

moderate expression: see section 11 12

4th ventricle lateral recess

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12

spinal cord ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9703
Entity Detected:	Dnajb13, DnaJ (Hsp40) related, subfamily B, member 13 ( MGI:1916637)
Sequence:	sense strand is shown >T9703 ACGCAAACTAACAGAGGCTGGAGTCTGAGGAAAAACAGACCCATTGGTACCAGCCATGGGGCTGGATTAC TATGCTGTGCTTCAGGTCACCCGCAACTCAGAAGATGCCCAGATCAAGAAGGCATACCGGAAACTTGCCC TGAAGAACCACCCATTGAAGTCATCTGAGCCGGGTGCACCAGAGATATTCAAGCAAATAGCAGAAGCCTA CGACGTGCTGAGCGACCCTGTGAAGCGAGGCATCTATGACAAGTTTGGTGAGGAAGGCCTGAAGGGTGGG ATTCCCCTAGAGTTTGGATCCCAGACTCCATGGACAACTGGCTACGTCTTCCATGGCAACCCTGACAAAG TGTTCCACGAGTTTTTTGGGGGAGACAACCCCTTCAGCGAATTTTTTGATGCAGAAGGAAATGATATAGA TTTGAATTTTGGGGGGCTTTGGGGCAGAGGCGTCCAGAAACAAGACCCTCCCATTGAACGAGACCTCTAT CTGTCCCTTGAGGACTTATTCTTTGGCTGCACCAAAAAAATTAAGATCTCCCGAAGGGTACTAAATGAAG ACAGATATTCTTCTACCATCAAGGACAAGATCCTGACCATCGATGTGAGACCTGGATGGAGGCAGGGCAC ACGCATCACCTTTGAGAAGGAAGGGGACCAGGGCCCCAACATCATCCCTGCTGACATTATCTTCATTGTG AAGGAGAAGCTGCACCCTCGCTTCCGCAGGGAGCATGACAACCTCTTCTTCGTGTACCCCATTCCTTTGG GCAAGGCCCTTACCTGCTGCACTGTGGAGGTGAAGACTCTTGATGACCGGCTGCTCAACATCCCTATCAA CGACATTGTCCACCCTAAGTACTTCAAGATTGTGCCAGGTGAGGGGATGCCACTGCCTGAGAACCCCAGC AAGAAGGGAGACCTCTTCATCTTCTTTGACATCCAGTTCCCCACCCGCCTCACACCCCAGAAGAAGCAGA TGCTGCGCCAGGCATTGCTGACCTAATTCTGGGGGACCTGAGCAGGAACAGGGATGGAGAGGGCTAGCTG GGCCTTGCCTGCTCCTATCCACTCAACTCCCACAGCCTATGAAGATGGGGAGGCGTGAGACCTTAGGCTG ACATAATAAAGATTCCTATCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6705915 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6705915 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006246 same experiment
	EMAGE:29258 same embryo
	EMAGE:29247 same embryo
	EMAGE:30852 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source