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EMAGE:30847

Ppfia4 ( MGI:1915757)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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euxassay_009512_01 euxassay_009512_02 euxassay_009512_03 euxassay_009512_04 euxassay_009512_05
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euxassay_009512_06 euxassay_009512_07 euxassay_009512_08 euxassay_009512_09 euxassay_009512_10
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euxassay_009512_11 euxassay_009512_12 euxassay_009512_13 euxassay_009512_14 euxassay_009512_15
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euxassay_009512_16 euxassay_009512_17 euxassay_009512_18

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
extraembryonic component
moderate moderate
regionalmoderate expression: see section 07
spinal cord
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14
brain
moderate moderate
regionalmoderate expression: see section 01 02 03 05 06 07 08 09 10 11 12 13 14 15 16 17 18 weak expression: see section 04
tail paraxial mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08
tongue muscle
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10
diaphragm
moderate moderate
regionalmoderate expression: see section 02 03 04 05 07 08 09 10 11 12 13 14 15 16 17 18
foot
moderate moderate
regionalmoderate expression: see section 01 11 12 13
eye skeletal muscle
moderate moderate
regionalmoderate expression: see section 02 04 13 14 15 16 17 18
leg muscle
moderate moderate
regionalmoderate expression: see section 02 03 04 10 11 12 13 14 15 16
vertebral axis musculature
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 08 09 10 11 12 13 14 15 16 17 18 weak expression: see section 01
arm muscle
moderate moderate
regionalmoderate expression: see section 03 04 17 18
lower leg rest of mesenchyme
moderate moderate
regionalmoderate expression: see section 15 16 17 18
hand
moderate moderate
regionalmoderate expression: see section 17 18
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36885
Entity Detected:Ppfia4, ( MGI:1915757)
Sequence:sense strand is shown

>T36885
GAAATTGGATGACGGAGAAGAGAAGGTGTTCCGCCGAGCACCCTCCTGGAGGAAACGCTTCCGGCCTCGG
GATCACCATAGCGGTGGCATGTTGGGCACCTCAGCGGAGACCCTCCCGGCGGGCTTCCGTGTGTCCACGC
TGGGTCCGCTGCAGCCTCCACCTGCCCCGCCTAAGAAGATCATGCCGGAAGCTCACTCCCACTATCTCTA
CGGACACATGCTCTCCGCCTTCCGGGACTAGCCACGGCCCCCAGGGCGGGTGTCCTGGTTTCACAGGCTC
AGCCGGCCCTGACCCTTCCTGCTCGTTCCCTTTCTCCCTTGGCTCCCAGTCTCTCCTCTGTGATTTTCCA
GTTGCCCGGGATCTCAGAATATGCTCATCCACTCCTTCATCATCCCACTATCCGAGTCCTACTTCGTTCC
CCCTTTGTAAGTCTCTGGAGGATGTGACCAAGGTCTCCCGTGAGTGTCTGTCGGGCACCCAGGTTGTAGG
TGTTTGAGATTCTGTAGGAGGGAGGGAAGGACAGCACCTGTGGCCACCTTCCCTCTTCCGGATCTGTTTT
GGTCTGGACTATGGGCTCATCAGGCCCAGGATCCCGGACCAAAGACTGAGATGACCCTTCCAATAAAAGG
CCATTTCGGACTCTGTCCTGCTTGGACTAATTGGCCTTCTGGACCCCTCCACTCTGGCCGTACCCGATGG
TCTGAGTGGTTTTGCCACCTCCTCTCTCAGTATCACTCCCTCCTCCCCAAACCCCAATCTCCAGTGTCTG
TAGCTCTTGCCTGCAAGTCTTGCTTGTGGGGTGACGAGGGGTGTAGAATACCCCGAACCCTCTTCCAGGA
CAAAGCCAGGAGTGGAGCTGAGGAAGACTAA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 79623. Forward Primer - name:079623_F_cDNA_Ppfia4, sequence:GAAATTGGATGACGGAGAAGAG; Reverse Primer - name:079623_N_SP6_cDNA_Ppfia4, sequence:TTAGTCTTCCTCAGCTCCACT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_009512 same experiment
 EMAGE:30527 same embryo
 EMAGE:32160 same embryo
 EMAGE:30161 same embryo
 EMAGE:30733 same embryo
 EMAGE:29745 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS