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EMAGE:30860

Cstb cystatin B ( MGI:109514)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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euxassay_009738_01 euxassay_009738_02 euxassay_009738_03 euxassay_009738_04 euxassay_009738_05
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euxassay_009738_06 euxassay_009738_07 euxassay_009738_08 euxassay_009738_09 euxassay_009738_10
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euxassay_009738_11 euxassay_009738_12 euxassay_009738_13 euxassay_009738_14 euxassay_009738_15
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
regionalmoderate expression: see section 10 11 13 14 15 weak expression: see section 12
maxilla
strong strong
single cellstrong expression: see section 05 06 07 09 10
mandible
strong strong
single cellstrong expression: see section 05 06 07 08 09 10
stomach
moderate moderate
regionalmoderate expression: see section 01 02 05 06 weak expression: see section 03 04
bladder
weak weak
regionalweak expression: see section 12 13 14
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30600
Entity Detected:Cstb, cystatin B ( MGI:109514)
Sequence:sense strand is shown

>T30600
AGGCACCCAGGTCTCCTAGTTGGATCTGTCTTCAGCTTCTCCGTGCTACCCCGACTACTGCTGCCAAGAT
GATGTGTGGCGCGCCATCTGCCACAATGCCAGCCACGGCCGAGACGCAGGAGGTCGCCGACCAGGTGAAG
TCCCAGCTTGAATCGAAAGAAAATCAGAAGTTTGATGTCTTTAAAGCCATATCCTTCAAGAGACAGATAG
TGGCTGGCACCAACCTCTTCATCAAGGTTGATGTTGGTGGAGATAAATGCGTGCACTTGAGGGTGTTTCA
ACCCCTCCCCCATGAAAACAAGCCTTTGACCCTGTCTTCCTATCAGACCAACAAAGAAAGGCACGATGAG
CTCTCCTACTTCTGATTCGGGGCTCCTTTGCACCTGTGTTTGGGACCACGTAGTAAAACCCCCCTCGGTG
ATGGAGAGAAGGGAGTTGAGCAGCTTCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5713930), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59742. Forward Primer - name:059742_F_IRAV114_c07_Cstb, sequence:AGGCACCCAGGTCTCCTA; Reverse Primer - name:059742_R_SP6_IRAV114_c07_Cstb, sequence:CAGAAGCTGCTCAACTCCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_009738 same experiment
 EMAGE:29957 same embryo
 EMAGE:29978 same embryo
 EMAGE:29958 same embryo
 EMAGE:30037 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS