EMAGE mouse gene expression spatial database

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EMAGE:30869

E330036I19Rik ( MGI:2442032)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004046_01	euxassay_004046_02	euxassay_004046_03	euxassay_004046_04	euxassay_004046_05

euxassay_004046_06	euxassay_004046_07	euxassay_004046_08	euxassay_004046_09	euxassay_004046_10

euxassay_004046_11	euxassay_004046_12	euxassay_004046_13	euxassay_004046_14	euxassay_004046_15

euxassay_004046_16	euxassay_004046_17	euxassay_004046_18	euxassay_004046_19	euxassay_004046_20

euxassay_004046_21	euxassay_004046_22	euxassay_004046_23	euxassay_004046_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

liver

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

spinal cord

weak

regional

weak expression: see section 06 07 08 09 10 11 12 not examined expression: see section 13 14

brain

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4404
Entity Detected:	E330036I19Rik, ( MGI:2442032)
Sequence:	sense strand is shown >T4404 CCTTGGCGCTGCTGGGGACCCCCTCCAGACACGCGCGGAGTCAGAAGGGTGCGCGCGGCTTAGGCGGCGC GGTGGGAGGTGCGCCATGCCGGGGATGGTGCTGTTCGGCCGGCGCTGGTCTCTAGCCAGCGACGACTTGG TGTTCCCCGGGTCTTTTGAGCTGTTCCTGCGTGTGCTGTGGTGGATTGTCAGTCTGACGTTGTATCTCAC GCACAGAAGGAGGCTGGACTGTCCCGGTGGAGTCTTGCTCAGCACGTACCTGATAGTCCTCCTTGTTCTC CTGGCAGTTATTATATGCACCGTGTTGGCCATCGTGTGTGTCAGCATGAGAGGAACCATTTGTAACCCTG GACCTCGGAAGTCTATGTCTAAGCTGCTCTATATCCGCCTGGCGCTCTTCCTGCCAGAGATGGTCTGGGC TTCTCTGGGGGCCGCCTGGGTGGCAAAAGGTATCCAGTGTGACAGGACAGTTGTCATTGGCATCATCGCC ACCGTCATTGTCAGCTGGATTGTCATTGCTGCCACCATGGTCACCATCATCTTTGTCTTTGACCCGCTGG GCGGGAAGATGGCTCCGTATCCCCCATGCATCCCGGAGCACCTGGACAGTAACAGCTCAAACCGGTTACT GACTGGCCTGAAGACGGCTGCGAAGAGCGTGTGGGAGACCCGGGTGCAGTTCTGCTGCTGCTGCGTTGGG CAGGATGATAACACCAGGGTGGCGTTTTCCAGCACTGCCGACCTGTTCTCCACCTACTTCTCTGACACAG ACCTGGTGCCTAGTGATATCGCAGCAGGCTTCACCCTTCTCCACCAGCAACAAGACAATATCAGCCACAG CCGGGAGCCTCCGGAGGTCGTGACCCACACACCAGGACAGCCTCAGGAAACTGAGTTAGATGCAGAAGTG GAGAACTGCCATCATTACATGCCGTTTGCAGCAGCTGCCTATGGGTGGCCGCTCTACATCTACAGGAACC CGTTCACGGGGCTGTGCAGGATTGGTGGCGACTGTTGCAGAGCCAGAGACATAGAGTACGATGCAGTGGA AGGTGACCAGCACAACTGCCACTTCGCCTCCATCCTGAAGACCACAGGGCTGCAGTACAGGGATTTCATT CACATAAGCTTTCACGACAAGGTGTATGAGCTGCCCTTCATAGTGGTTCTGGACCACAGGAAGGAGTCTG TTGTGGTCGCCGTGAGAGGGACCATGTCTCTCCAGGACGTCCTGACCGACCTGTCTGCCGAGAGTGAGAC CCTGGAGCTGGGGATTGAGCTACAGGACTGTGTGGCCCACAAGGGAATTGCTCAAGCGGCCAGATACATT CACCGCAGACTGGTCAATGATGGGATTCTGAGCCAAGCCTTCAGTGTCGCTCCGGAGTACCAGCTCGTTC TGGTGGGGCACAGCCTAGGAGCCGGCGCTGCGGCCCTGTTAGCCATCATGCTCCGGGGGGCCTACCCACA AGTCCGTGCTTATGCCTTCTCCCCGCCCAGGGGGCTGCTCAGCAAATCCCTTTACGAGTACTCCAAGGAC TTTGTCGTGTCGCTTATCCTAGGGATGGATGTGATCCCCAGGTTAAGTGTGACCAACATGGAGGACCTCA AGAGGAGGATCCTGAGAGTGATCGCTAACTGCAATAAGCCGAAGTACAAGATCTTGCTGCATGGCTGTTG GTACGGACTGTTCGGAGGAAGCCCTGATAACTTTCCAACTGAACTGGATGAGGGCACCCAGGGGGCCCTG ACTCAGCCTCTTCTTGGAGAGCAGACACTTCTAACCCGATACTCCCCGGGCTACTGTTCCAGCGACTCCC CACTGGACTCCCCCACCAAGTACCCCACTCTGTACCCACCTGGCAGGATCATCCACCTGGAAGAGGAGGG TGGTTCAGGGAGGTTTGGCTGCTGTTCTGCTGCCCAGTACAGAGCGAGGTGGGCACACGAAGCAGAATTC AGTAAGATCCTGATAGGCCCAAAGATGCTGATTGACCACATGCCTGACGTCATGATTCGGGCCCTGGACA GAGTGCTTGCCGACAGGACAGCCTGCGTCTCCTGCCCAGGGCAAGGCGGCTCAAGTGTACCGTAGTCAGC TCTACTGGACCCTGACCGGTACAACGGACTGGCAGTTTCAGGAGGTTTTTTTGTTGTTGCTTTTGTTTTT TTCTTAAGACTGATTGACTGTGCGTGGCTTCTGTGAGGCTGGTATGGTGGTATCTGTCTATCAACAGTTA CCTGAGGGGTCCCTGCAGAAATGGACACCAAAATGACCATACAATTTATACAACCTTTAAAAAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4921222 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4921222 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004046 same experiment
	EMAGE:30019 same embryo
	EMAGE:29969 same embryo
	EMAGE:30890 same embryo
	EMAGE:30882 same embryo
	EMAGE:29984 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source