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EMAGE:30876

Gchfr ( MGI:2443977)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
medulla oblongata basal plate marginal layer
strong strong
single cellstrong expression: see section 10 11 12 13 14 moderate expression: see section 09
midbrain mantle layer
strong strong
single cellstrong expression: see section 12 13 14 15
pons marginal layer
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8322
Entity Detected:Gchfr, ( MGI:2443977)
Sequence:sense strand is shown

>T8322
GGCACGAGGGTGGTCGCGGGCAGCCCCAGCCACGCACCATGCCCTATCTGCTCATCAGCACTCAGATCCG
TATGGAGGTGGGCCCCACCATGGTGGGTGATGAACACTCGGATCCGGAGCTGATGCAGCACCTGGGGGCC
TCCAAGAGAAGTGTCTTGGGAAACAACTTTTACGAATACTACGTCAACGACCCTCCTCGCATAGTCCTGG
ACAAGCTGGAATGCAAGGGCTTCCGAGTGCTGAGCATGACAGGGGTGGGCCAGACGCTGGTGTGGTGTCT
GCACAAGGAATGAGCTGCTTGAGCTGACCTGTTCAGAGACCGCCATGTGCCCTGCCCACATCCTTGCATC
ACTGTTTGCCCTGCCTATCTCTTTCCAAATTGTCAACTCCCTTGCCCAATACTGGTGGGCTGTGAACAGC
CTAAGGGACCTCGCTCCAGCTGGTTGGGCACAGCAGGCTCCTGCCCTGGTGCTTTCAGCTTCCCCCAGCT
AGGCCTGACCCTGGCTCTTGCTGTAGGCCACATGCTGAAGGCAAGCCAACCCGAGGACGAGGACAGTCCC
TGGATCCTACACAGCTAATCCAAGCCAATAAAGGGCTCTCACAAGTGAAAAAAAAAAAAAAAAAAAAAAA
AAAAGAAAC
Notes:The probe template was PCR amplified from IMAGE:1511037 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1511037 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_005374 same experiment
 EMAGE:30879 same embryo
 EMAGE:29970 same embryo
 EMAGE:29981 same embryo
 EMAGE:30006 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS