EMAGE mouse gene expression spatial database

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EMAGE:30878

Gdf5 growth differentiation factor 5 ( MGI:95688)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004285_01	euxassay_004285_02	euxassay_004285_03	euxassay_004285_04	euxassay_004285_05

euxassay_004285_06	euxassay_004285_07	euxassay_004285_08	euxassay_004285_09	euxassay_004285_10

euxassay_004285_11	euxassay_004285_12	euxassay_004285_13	euxassay_004285_14	euxassay_004285_15

euxassay_004285_16	euxassay_004285_17	euxassay_004285_18	euxassay_004285_19	euxassay_004285_20

euxassay_004285_21	euxassay_004285_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

foot

strong

regional

strong expression: see section 05 06 07 09

arm

moderate

regional

moderate expression: see section 01

femur

strong

regional

strong expression: see section 08 09 17 18

hand

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09

rib

moderate

regional

moderate expression: see section 13 14 15

leg

moderate

regional

moderate expression: see section 01 02 03 04

axial skeleton

moderate

regional

moderate expression: see section 10 11 16 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6369
Entity Detected:	Gdf5, growth differentiation factor 5 ( MGI:95688)
Sequence:	sense strand is shown >T6369 TGGAGGCCTGGGAACGGGGCCGGGCCGTGGACCTCCGTGGCCTGGGCTTTGAACGCACTGCCCGACAGGT CCACGAGAAAGCCTTGTTCCTAGTGTTTGGTCGTACCAAGAAACGGGACCTGTTCTTTAATGAGATTAAG GCCCGCTCTGGCCAGGATGACAAGACTGTGTATGAATATTTGTTCAGCCAGCGGCGGAAACGCCGGGCCC CATTGGCCAATCGCCAGGGCAAGCGACCCAGCAAGAACCTCAAGGCTCGCTGCAGTCGCAAGGCCTTGCA TGTCAACTTCAAGGACATGGGCTGGGACGACTGGATCATCGCACCTCTTGAGTATGAGGCCTTCCACTGC GAAGGACTGTGTGAGTTCCCCTTGCGCTCCCACTTGGAGCCCACAAACCACGCAGTCATTCAGACCCTAA TGAACTCTATGGACCCTGAATCCACACCACCCACTTGTTGTGTGCCTACACGGCTGAGTCCTATTAGCAT CCTCTTCATCGACTCTGCCAACAACGTGGTGTATAAACAGTACGAGGACATGGTCGTGGAATCTTGTGGC TGCAGGTAGCAGCACTGGCCCACCTGTCTTCCAGG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers.. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004285 same experiment
	EMAGE:32164 same embryo
	EMAGE:29917 same embryo
	EMAGE:29772 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source