EMAGE mouse gene expression spatial database

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EMAGE:30879

Fhad1 ( MGI:1920323)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005373_01	euxassay_005373_02	euxassay_005373_03	euxassay_005373_04	euxassay_005373_05

euxassay_005373_06	euxassay_005373_07	euxassay_005373_08	euxassay_005373_09	euxassay_005373_10

euxassay_005373_11	euxassay_005373_12	euxassay_005373_13	euxassay_005373_14	euxassay_005373_15

euxassay_005373_16	euxassay_005373_17	euxassay_005373_18	euxassay_005373_19	euxassay_005373_20

euxassay_005373_21	euxassay_005373_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 10 11

nasal cavity olfactory epithelium

moderate

single cell

moderate expression: see section 09 10 11 12 14 15 16

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

ventral grey horn

strong

regional

strong expression: see section 10 11 12 13 14 moderate expression: see section 09 15 16

4th ventricle

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

pons ventricular layer

strong

regional

strong expression: see section 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8321
Entity Detected:	Fhad1, ( MGI:1920323)
Sequence:	sense strand is shown >T8321 TTTGTCTAGGAGATAGAATTAAAGGAGCAGAAAGAAAACGTCTTAAATAATAAATTAAAAGACGCCCTGG TCATGGTAGAGGACGCTCAGCAGATGAAGACAACGGAGAGTCAAAGAGCCGAGACGCTCGCTCTGAAGTT AAAGGAAACATTAGCAGAGCTAGAAACAACCAAGACGAAAATGATCCTGACGGACGATCGGCTGAAGCTG CAGCAGCAGTCGATGAAGGCCCTGCAGGATGAGCGGGAATCCCAGAAGCACGGCTTCGAGGAGGAAATCT CAGAGTACAAAGAGCAGATCAAACAGCACTCGCAGACCATCGTGAGCCTAGAGGAAAGGCTCTGCCAAGT GACTCAGTACTACCAGAAGATAGAAGGGGAGATCACAACGTTGAAAAACAACGATACAGGACCGAAGGAG GAAGCCTCCCAAGACCTTACAGCAGGACCCCCCCTGGACAGTGGTGACAAAGAAATAGCGTGCGACCATC TGATAGATGACTTACTAATGGCTCAGAAGGAAATCCTATCCCAGCAGGAGATAATCATGAAGCTGAGGAC TGACCTCGGGGAAGCTCACAGCCGGATGTCAGACCTGAGAGGGGAACTAAGCGAGAAGCAGAAGATGGAG CTGGAGCGGCAGGTGGCCCTGGTGCGACAGCAGAGCGGGGAGCTGAGCATGCTGAAGGCGAAGGTGGCGC AGACGACTGGCCTGATGGAGAAGAAGGACAGGGAACTGAAGGTCCTCAGGGAGGCGCTCAGGTGTGGGAA GGGGTCCGTGTGACGTGACCACCCCAAGGCCCTGGCCCTGGCACTGGCACAGAGAGGGCTCAGTAAATGC TGGCTGAAGCGGACTGATACATGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1496088 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1496088 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005373 same experiment
	EMAGE:29970 same embryo
	EMAGE:29981 same embryo
	EMAGE:30876 same embryo
	EMAGE:30006 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source