Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30880

Gsta4 glutathione S-transferase, alpha 4 ( MGI:1309515)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:30880 EMAGE:30880 EMAGE:30880 EMAGE:30880 EMAGE:30880
euxassay_006220_01 euxassay_006220_02 euxassay_006220_03 euxassay_006220_04 euxassay_006220_05
EMAGE:30880 EMAGE:30880 EMAGE:30880 EMAGE:30880 EMAGE:30880
euxassay_006220_06 euxassay_006220_07 euxassay_006220_08 euxassay_006220_09 euxassay_006220_10
EMAGE:30880 EMAGE:30880 EMAGE:30880 EMAGE:30880 EMAGE:30880
euxassay_006220_11 euxassay_006220_12 euxassay_006220_13 euxassay_006220_14 euxassay_006220_15
EMAGE:30880 EMAGE:30880 EMAGE:30880 EMAGE:30880 EMAGE:30880
euxassay_006220_16 euxassay_006220_17 euxassay_006220_18 euxassay_006220_19 euxassay_006220_20
EMAGE:30880
euxassay_006220_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
olfactory cortex ventricular layer
weak weak
regionalweak expression: see section 12 13 17 18
right lung
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15 16 weak expression: see section 17 18
pituitary gland
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14
medulla oblongata basal plate ventricular layer
weak weak
regionalweak expression: see section 05 06 07 08 09 10 11 12
stomach
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08
pons ventricular layer
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16
urethra of male
moderate moderate
regionalmoderate expression: see section 10 11
left lung
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09
cochlea
moderate moderate
regionalmoderate expression: see section 01 02 03 05 06 07 15 16 weak expression: see section 04 17 18
spinal cord ventricular layer
strong strong
regionalstrong expression: see section 07 08 09
bladder
strong strong
regionalstrong expression: see section 09 10 11
esophagus
strong strong
regionalstrong expression: see section 09 10
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 06 07 08 16 17 18
trachea
moderate moderate
regionalmoderate expression: see section 10 11
thymus primordium
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12
renal cortex
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 12 13 14 15 16 not examined expression: see section 07
posterior naris
strong strong
spottedstrong expression: see section 15 17
telencephalon ventricular layer
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21
midbrain ventricular layer
weak weak
regionalweak expression: see section 05 06 07 08 09 10 11 12
nasal cavity olfactory epithelium
strong strong
spottedstrong expression: see section 10 11 13 14 16 17 18 19 20 moderate expression: see section 09 12
hypothalamus ventricular layer
moderate moderate
regionalmoderate expression: see section 13 14 weak expression: see section 11 12
lateral ventricle choroid plexus
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18
4th ventricle
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 13 weak expression: see section 10 11 12
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T693
Entity Detected:Gsta4, glutathione S-transferase, alpha 4 ( MGI:1309515)
Sequence:sense strand is shown

>T693
TCCTCGAGNCTGTTGGCCTACTGGAGAACCGCTTCTTTCCAGTGCCTGGAGACAACAATCCCACAAGAAT
AAGGAAGCTCTGAAGCAGGAGTCATGGCAGCCAAACCTAAGCTCTACTACTTTAATGGCAGGGGACGGAT
GGAGTCGATCCGCTGGCTGCTGGCTGCGGCTGGAGTGGAGTTTGAGGGAGAATTTCTTGAGACAAGGGAA
CAGTATGAGAAGATGCAAAAGGATGGACACCTGCTTTTCGGCCAAGTACCCTTGGTTGAAATCGATGGGA
TGATGCTGACACAGACCAGGGCCATCCTCAGCTACCTCGCTGCCAAGTACAACTTGTATGGGAAGGACCT
GAAGGAGAGAGTCAGGATTGACATGTATGCAGATGGCACCCAGGACCTGATGATGATGATTGCCGTGGCT
CCATTTAAAACCCCCAAGGAAAAAGAGGAGAGCTATGATTTGATACTGTCAAGAGCTAAAACCCGTTACT
TCCCAGTGTTTGAAAAGATTTTAAAAGACCACGGAGAGGCTTTTCTCGTTGGCAACCAGC
Notes:The probe template was PCR amplified from IMAGE:1889069 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1889069 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006220 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS