EMAGE mouse gene expression spatial database

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EMAGE:30882

Bbox1 ( MGI:1891372)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004044_01	euxassay_004044_02	euxassay_004044_03	euxassay_004044_04	euxassay_004044_05

euxassay_004044_06	euxassay_004044_07	euxassay_004044_08	euxassay_004044_09	euxassay_004044_10

euxassay_004044_11	euxassay_004044_12	euxassay_004044_13	euxassay_004044_14	euxassay_004044_15

euxassay_004044_16	euxassay_004044_17	euxassay_004044_18	euxassay_004044_19	euxassay_004044_20

euxassay_004044_21	euxassay_004044_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

liver

weak

regional

weak expression: see section 01 02 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4953
Entity Detected:	Bbox1, ( MGI:1891372)
Sequence:	sense strand is shown >T4953 ATCAGGTTCAGGTACAATCTGCCAGCCTACTATAATTAACACTGATTTGTCCTAGCAGGTGGCTGATAGG ATTTATCACCAAAGACCAGAAACATGCACTGTGCCATCCTAAAGGCAGAAGCAGTGGATGGGGCTCATTT GATGCAGATCTTTTGGCACGATGGGGCAGAGTCCCTCTACCCAGCAGTATGGCTGAGAGACAACTGTCAG TGTTCAGACTGCTACCTACATTCTGCAAAAGCTCGGAAACTTCTCCTGGAAGCTCTTGATGTAAACATTA GAATCGATGATTTGACATTTGACCGGAAAAAGGTTTATATCACATGGCCCAATGACCATTACAGTGAATT CGAAGCTAACTGGCTGAAGAAAAGATGCTTTTCTCAACAGGCCAGAGCAAGGCTCCAAGGAGAATTGTTT TTACCAGAATGTCAGTACTGGGGCTCAGAACTCCAGCTGCCAACTCTGAATTTTGAAGATGTGTTAAATG ATGATGACCATGCATACAAATGGCTTTCCAGTCTCAAGAAAGTAGGCATTGTGAGACTCACCGGAGCAGC TGACAAACGTGGTGAGATCATAAAACTTGGGAAAAGAATTGGCTTCCTGTACCTCACATTTTATGGACAC ACTTGGCAAGTGCAGGACAAGATTGATGCCAACAATGTGGCTTATACAACTGGGAAGCTCAGCTTCCACA CTGACTACCCAGCCCTCCACCATCCACCTGGGGTTCAGCTTCTGCATTGTATCAAGCAAACAGTCACAGG AGGTGACTCTGAAATTGTGGATGGGTTTAACGTGTGCCAAAAGCTCAAGGAGAAGAACCCTCAGGCTTTC CATATTCTCTCCTCGACCTTTGTGGATTTTACAGACATTGGAGTGGATTACTGTGATTTCTCTGTGCAAT CTAAACACAAGATTATAGAGTTGGATGATAAAGGCCAAGTGGTTCGCGTCAACTTCAACAATGCAACCAG AGATACAGTCTTTGATGTCCCCATCGAAAGAGTTCAGCCTTTCTACGCTGCTCTGAAAGAGTTTGTTGAC CTCATGAACAGCAAAGAATACAAGTATACCTTCAAGATGAATCCAGGTGATGTGATTACCTTCGACAACT GGCGCTTGCTTCATGGACGTCGTAGCTATGAGGCGGGAACTGAGATATCCCGCCATCTGGAAGGAGCGTA TGCTGACTGGGATGTAGTGATGTCAAGGCTTCGTATTCTGAGACAGAGAGTCACGAATGGGAACTGAATC TCCTGGCCTTAGACTTTAGTGCAAGTCCAGTGACTGAATGCTCCTATCTATAACCTGGTTATTCTCATAT CATGATCAACATCATTCACATAATAATTTCTTTAAAACTGAACATGCAGCTTCTCTCATCAGGGTATTCT TCCCTGCAATCATATTCAATATCTCTTATTTAGTGTCATGGTAAGGGTTAGTCTCAAAGCATCCCTTCTG TGGGATAAGCTCTCTGACTTTGCATTAGCAAGTATTACCAAACCACCCCCCCTACAAATAGTTTTATATC TAACAAATCTTTCTGGCAAATAAAACTGTTTTATAAATGATTAAAGCTTGTGTTTCAAATAAAAGATCTT AAAATATGTTCCTGCTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5134018 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5134018 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004044 same experiment
	EMAGE:30869 same embryo
	EMAGE:30019 same embryo
	EMAGE:29969 same embryo
	EMAGE:30890 same embryo
	EMAGE:29984 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source