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EMAGE:30890

Wnt10a wingless related MMTV integration site 10a ( MGI:108071)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:30890 EMAGE:30890 EMAGE:30890 EMAGE:30890 EMAGE:30890
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EMAGE:30890 EMAGE:30890 EMAGE:30890 EMAGE:30890 EMAGE:30890
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euxassay_004059_21 euxassay_004059_22 euxassay_004059_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
lower jaw molar
strong strong
regionalstrong expression: see section 07 weak expression: see section 20
upper jaw molar
strong strong
regionalstrong expression: see section 07 weak expression: see section 20
epidermis
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 23 weak expression: see section 01 02 03 04 21 22
lower jaw incisor
strong strong
regionalstrong expression: see section 12 moderate expression: see section 13 15 16
upper jaw incisor
strong strong
regionalstrong expression: see section 12 17 moderate expression: see section 13 16
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4406
Entity Detected:Wnt10a, wingless related MMTV integration site 10a ( MGI:108071)
Sequence:sense strand is shown

>T4406
GGCGGGCGCCGTCTGCTGCGGGAGCTGTGACCTGAGTAGGAGCTGTGTGTCGCAGCCGCCCCACCCCTGC
CGATCATGCGCCGGCGACCCTGGTTCGCCAGTCCCACTGGGCTGTGAGCCCCCCACTCCTGGCCTGTCAC
GGCCCGCGCGCCATGGGCAGCGCCCACCCTCGCCCCTGGCTGCGGCTCCCACAAGGGCCCCAGCCGCGGC
CTGAGTTCTGGGCGCTCCTGTTCTTCCTACTGCTGCTGGCTGCCGCTGTGCCCAGGTCAGCACCCAACGA
CATCCTGGGCCTCCGCCTACCCCCAGAGCCCGTGCTCAACGCCAACACAGTGTGCCTGACATTGCCCGGC
CTGAGCCGGCGGCAGATGGAGGTGTGTGTGCGTCACCCTGACGTGGCCGCCTCTGCTATCCAGGGCATCC
AGATCGCCATCCATGAGTGCCAGCATCAGTTCCGGGACCAGCGCTGGAACTGCTCCAGCCTGGAGACTCG
GAACAAAGTCCCCTACGAGAGCCCCATCTTCAGCCGAGGTTTTCGAGAGAGTGCTTTCGCCTACGCCATA
GCAGCTGCCGGGGTGGTGCACGCAGTGTCCAACGCGTGCGCTCTGGGTAAACTGAAGGCTTGCGGTTGCG
ACGCCTCCAGACGTGGGGACGAAGAAGCTTTCCGTCGGAAGCTGCACCGCTTGCAGCTGGACGCGCTGCA
GCGCGGAAAGGGCTTGAGCCACGGGGTCCCTGAACACCCGGCCATACTTCCTGCCAGCCCAGGTCTGCAG
GACTCCTGGGAGTGGGGTGGCTGCAGTCCGGATGTGGGCTTCGGAGAACGCTTCTCTAAGGACTTTCTGG
ACTCCCGAGAGCCTCACAGAGACATCCATGCTCGAATGAGACTCCACAACAACCGTGTGGGCCGGCAGGC
GGTGATGGAGAACATGCGGCGTAAGTGCAAATGCCACGGCACCTCAGGCAGCTGCCAGCTCAAGACCTGC
TGGCAGGTGACGCCTGAGTTCCGCACAGTAGGGGCGCTGCTGCGCAACCGCTTCCACCGCGCCACGCTCA
TCCGGCCGCACAACCGCAACGGTGGCCAGCTGGAGCCCGGCCCCGCGGGAGCACCCTCGCCAGCACCGGG
CACTCCAGGGCTGCGCCGCAGGGCCAGCCACTCCGACCTGGTCTACTTTGAGAAATCTCCCGACTTCTGT
GAGCGCGAGCCGCGCCTGGACTCGGCAGGCACTGTGGGCCGCCTGTGCAATAAGAGCAGCACGGGTCCCG
ATGGCTGCGGCAGCATGTGCTGTGGCCGCGGCCACAACATTCTGCGCCAGACGCGCAGCGAGCGCTGCCA
CTGCCGGTTCCACTGGTGCTGCTTCGTGGTCTGCGAAGAATGCCGCATCACCGAGTGGGTCAGCGTCTGC
AAGTGAGCAGACCCAAGCTCCTCTGGGTCTCAAGAATGGTTGTCCTCTTGGTGCCTGGCTTCTGCCGCTA
GCGGATCTGAGCCAGGCAGCAAGCAGCAGCCTTGGCTCCTGAGAGAGGTGGTTGGCTCTTACAGCCCCGA
GGGTCTACAATCACCAGACAGTCCAGATCTGATTGACATTCCTCCGCTCACCTCTGTAGGTTCCCCTCTT
TCTGTTCCTAGCTCAGACAGCTGGGGGTGATAGTGGAGACTGTTCCACACCCTAGGACAGGTCACCAAAG
CAGCCCAGCCTGGCATGCCTACCTCCTGTCATCTCTTCTTCCCTTCCCCAGGAGTGATAGGCAATGCACT
GAAGCTGATGGGCACCGGGGAAGAAAACTAAAAGGCAGAAATGGCCGTCATCGGGCTGAAGTGACTCTAA
GGGCTCCAGACCTCTGCTCCTGTCTTTCACTTAACAGATATTTATTTTTGCGCTCTCTTTGAGACACTCT
CTGGGGAAAAAGAAGCTCCGGAGTCTACAGGCTGATTAAGGGACATGGACAATAAACCAGTAAACACACA
AAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4921327 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4921327 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_004059 same experiment
 EMAGE:30869 same embryo
 EMAGE:30019 same embryo
 EMAGE:29969 same embryo
 EMAGE:30882 same embryo
 EMAGE:29984 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS