EMAGE mouse gene expression spatial database

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EMAGE:30892

Ptchd1 ( MGI:2685233)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006479_01	euxassay_006479_02	euxassay_006479_03	euxassay_006479_04	euxassay_006479_05

euxassay_006479_06	euxassay_006479_07	euxassay_006479_08	euxassay_006479_09	euxassay_006479_10

euxassay_006479_11	euxassay_006479_12	euxassay_006479_13	euxassay_006479_14	euxassay_006479_15

euxassay_006479_16	euxassay_006479_17	euxassay_006479_18	euxassay_006479_19	euxassay_006479_20

euxassay_006479_21	euxassay_006479_22	euxassay_006479_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 weak expression: see section 06 07 08

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 14 15 16

midbrain mantle layer

moderate

single cell

moderate expression: see section 10 11 12 13 14 weak expression: see section 07 08 09 15

ventral grey horn

moderate

single cell

moderate expression: see section 07 08 09 10 11 12

diencephalon lateral wall mantle layer

moderate

single cell

moderate expression: see section 10 11 12 13 14 weak expression: see section 08 09 15

rest of cerebellum mantle layer

strong

regional

strong expression: see section 09 10 12 13 moderate expression: see section 03 04 05 06 07 08 14 15 16 17 18 19

pons mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 weak expression: see section 05 06 07 08

dorsal grey horn

moderate

single cell

moderate expression: see section 09

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35790
Entity Detected:	Ptchd1, ( MGI:2685233)
Sequence:	sense strand is shown >T35790 TCCTGGAAGAGCTAAAGAATGCTCGGGCCACCAATCGGACCAATTTTGCTATCACATATCCGATCACTCA CTTAAAAGATGGAAGGGCTGTGTATAATGGGCACCAACTCGGAGGTGTCACTGTGCACAGCAAGGATCGG GTGAAATCTGCAGAGGCCATTCAGCTTACCTACTATCTGCAATCAATCAACAGTCTCAATGACATGGTAG CTGAGAGGTGGGAGTCCAGCTTCTGTGACACTGTCAAACTATTCCAGAAATCCAACAGCAAAGTCAAAAT ATACCCGTACACATCCTCATCACTGAGGGAAGATTTCCAGAAGACCAGCCGTGTATCAGAACGTTACCTG GTCACCAGCCTGATCCTGGTAGTCACCATGGCTATTCTGTGCTGCTCTATGCAGGACTGTGTCCGCAGCA AACCCTGGTTAGGCCTACTTGGGTTGGTGACCATAAGCCTAGCCACTCTCACTGCAGCCGGAATCATCAA TCTCACTGGTGGAAAATACAACTCCACCTTCCTGGGAGTCCCTTTCGTCATGCTAGGTCATGGATTATAC GGGACTTTTGAAATGTTATCCTCGTGGAGGAAAACTAGAGAGGACCAACATGTTAAAGAGCGAACTGCAG AAGTCTATGCAGACTCCATGCTCTCCTTCTCTCTCACCACTGCCATGTACTTGGTCACCTTCGGCATCGG GGCCAGCCCTTTCACAAACATTGAGGCAGCCAGGATTTTCTGCTGCAACTCCTGTATTGCAATCCTCTTC AACTACCTCTATGTACTCTCATTTTATGGTTCCAGCCTTGTGTTCACTGGCTACATAGAAAACAATTACC AGCATAGTATATTCTGTAGAAAAGTTCCGAAGCCTGATGTATTACAGGAGAAGCCAGCATGGTATAGGTT T
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 86966. Forward Primer - name:086966_F_cDNA_9630036J22Rik, sequence:TCCTGGAAGAGCTAAAGAATGC; Reverse Primer - name:086966_N_SP6_cDNA_9630036J22Rik, sequence:AAACCTATACCATGCTGGCTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006479 same experiment
	EMAGE:31312 same embryo
	EMAGE:29960 same embryo
	EMAGE:29962 same embryo
	EMAGE:29963 same embryo
	EMAGE:31302 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source