EMAGE mouse gene expression spatial database

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EMAGE:30903

9430076C15Rik RIKEN cDNA 9430076C15 gene ( MGI:2443551)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_016017_26

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

oral epithelium

weak

regional

weak expression: see section 06 07 21

hindlimb digit 3 mesenchyme

weak

regional

weak expression: see section 07 08 17 18

telencephalon ventricular layer

weak

regional

weak expression: see section 05 06 07 08 15 16 17 18 19 20

hindlimb digit 5 mesenchyme

weak

regional

weak expression: see section 07 18 19

hindlimb digit 1 mesenchyme

weak

regional

weak expression: see section 06 17 18

hindlimb digit 4 mesenchyme

weak

regional

weak expression: see section 07 17 19

hindlimb digit 2 mesenchyme

weak

regional

weak expression: see section 06 08 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39685
Entity Detected:	9430076C15Rik, RIKEN cDNA 9430076C15 gene ( MGI:2443551)
Sequence:	sense strand is shown >T39685 CATCAGACTCACGCAAGGATAGTGTCCTTCCCTCTGTCACAGTGATCCCCACAGTCCACAAACTTGGGGA CGTTCATGTATTTTGTCTGCACTTCACAGTTCTCATTTTGTAGAGCTGGCTCTTTTGACAAATTAGAGGC AGGAGGTGCTCACAGGCCACCCAGCCTGGAGGTGAGGTCAGAGTGACCCTCTTGAAAGGCCACCCGTTGT TACCTGCTCTGCTCTTGTTGACAGAGGGACATTTGGTGGCCTTTCTGAACCCTCTTGTGGCTTCCTATGT TCTTTACTTTCTGAAACCTTACCGGCTGTTTTATCCTGTCCGTGACTGTGGCGTTGTTACCCATGGCACA CACCTCTCTCCTTTGGCTCTCAAGTGGCATTCCCAAAGGACCTACGTCTCAGCATCACCTGAGCATCACC TCTCAGCATCACCTGAGCATCACCTCTCAGCATCACCTGAGCATCACCTCTCAGCATCACCTGAGCATCA CCTCTCAGCATCACCTGAGCACACCATTTCTGATAAACTCCAGCATGCATGTCAAGATGAGACCAGCCTG TAAGATCAGCCAATCATTTTGGAGCAATAAGATGGGTGATCCACTTACTAGCAAGCCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 69442. Forward Primer - name:069442_F_cDNA_9430076C15Rik, sequence:CATCAGACTCACGCAAGGATAG; Reverse Primer - name:069442_N_SP6_cDNA_9430076C15Rik, sequence:AGGCTTGCTAGTAAGTGGATC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_016017 same experiment
	EMAGE:30883 same embryo
	EMAGE:30003 same embryo
	EMAGE:30044 same embryo
	EMAGE:30039 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source