EMAGE mouse gene expression spatial database

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EMAGE:30905

Acsm1 acyl-CoA synthetase medium-chain family member 1 ( MGI:2152200)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

lung

strong

regional

strong expression: see section 09 10 moderate expression: see section 06 07 08 11 12 14 15 16 17 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4913
Entity Detected:	Acsm1, acyl-CoA synthetase medium-chain family member 1 ( MGI:2152200)
Sequence:	sense strand is shown >T4913 CTTACAAGCCGTCTCTCCTGGCTATAGTCTACCTGTGTCCTTCACAGTACCAGGACTGCCCTAATATGCA GTGGCTGAAGAGTTTCCAGATCTGCAAAGTCTTACAGGGCTTCTCCCTAAGCCCCACCCAGCTGCACAGA CGGTTGTTTTCAAGAGTTGGAGCTCCAAGATGGAATGACCATGATTCACCTGAGGAGTTTAACTTTGCAA GTGATGTCCTGGACTACTGGGCTCAAATGGAGGAGGAGGGCAAGAGAGGACCAAGTCCAGCCTTTTGGTG GGTGAATGGCCAAGGAGATGAAATAAAGTGGAGCTTCAGGAAGCTGAGGGACCTCACCTGTCGCACTGCC AACGTCTTTGAGCAGATTTGTGGCCTGCAGCAAGGAGATCACCTGGCCTTGATTCTGCCCCGAGTGCCCG AGTGGTGGTTGGTGACAGTGGGCTGCATGCGAACAGGGATCATCTTCATGCCTGGGACTACCCAACTGAA AGCCAAGGACATTCTCTACCGAATACAAATATCTCGAGCCAAAGCCATTGTGACCACAGCTAGCCTTGTC CCAGAGGTGGAATCTGTGGCTTCTGAGTGTCCTGATCTGAAAACCAAGCTGGTGGTGTCTGATCACAGCC ATGAAGGGTGGCTTGATTTCTGTTCACTGATTAAATCAGCATCCCCAGACCATACTTGTATTAAGTCAAA GATGAAGGATCCCATGGCCATCTTCTTCACCAGTGGGACCACAGGCTACCCCAAGATGGCAAAGCACAAC CAGGGACTTGCCTTCCGGTCATATATCCCTTCATGCAGAAAATTATTGAAGCTGAAGACATCTGACATCT TGTGGTGCATGTCAGACCCAGGATGGATTCTGGCTACCGTGGGGTGCCTGATCGAGCCATGGACATCAGG ATGTACAGTCTTCATCCACCACCTCCCTCAATTCGACCCCAAAGTCATTGTAGAGGTACTGTTCAAATAC CCCATCACTCAGTGCCTTGCTGCCCCAGGCGTGTATCGAATGGTTCTTCAGCAGAAAACCTCCAACCTCA GGTTCCCCACCCTTGAGCATTGCACTACTGGTGGGGAGAGCCTGCTGCCTGAGGAGTATGAGCAGTGGAA GCAAAGGACAGGTCTTTCCATCCACGAGGTCTATGGACAGTCAGAAACGGGGATCAGCAGTGCCACCCTC CGGGAAATGAAGATCAAGCGAGGCTCCATAGGGAAGGCCATCTTACCCTTTGACTTGCAGATCATCGATG AAAAGGGCAACATCCTCCCACCCAACACTGAAGGATACATTGGCATCAGGATCAAGCCCACCAGGCCTCT AGGCCTCTTCATGGAATATGAGAATAGCCCAGAGAGCACATCTGAAGTGGAGTGTGGGGACTTTTACAAT AGTGGGGATAGAGCGACCATTGATGAAGAGGGCTACATCTGGTTCTTGGGAAGGGGCGATGATGTCATCA ATGCTTCCGGGTATCGCATCGGGCCTGTAGAGGTGGAGAACGCCTTGGCGGAGCACCCAGCAGTGGCAGA GTCTGCGGTGGTGAGCAGCCCGGACAAGGATCGAGGAGAGGTGGTGAAGGCGTTTATTGTCCTCAACCCA GAGTTCCTGTCACACGATCAGGAACAGCTTATCAAAGAGCTACAGCATCATGTGAAGTCAGTGACGGCAC CATACAAGTACCCCAGGAAGGTGGAGTTTGTTTCAGAATTGCCCAAAACTGTCACAGGCAAAATCAAAAG GAAGGAACTTCGAAACAAGGAGTTTGGTCAGCTATGAACAGCAATCAACCCAGAGCATCCTGTGACCTGA ATCCTTGGCTGTTGGGGTCAGGGTGGTGTGGGATGACGATGGAATGCAGGAAGGGTAAATGTGGAAGAGA AAACCAAGACCCCAACATTTTTGCTTTCTTCAAACTCAGTTGGTCCCCTTCCTGGATACCTTCTTCTTCC CCTTCCACAGTGCACTCTCAGACTGTTTGTGGACAATAAAAATACTGACTTGAATAGCAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:5102652 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5102652 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003170 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source