EMAGE mouse gene expression spatial database

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EMAGE:30908

Liph lipase, member H ( MGI:2388029)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003172_01	euxassay_003172_02	euxassay_003172_03	euxassay_003172_04	euxassay_003172_05

euxassay_003172_06	euxassay_003172_07	euxassay_003172_08	euxassay_003172_09	euxassay_003172_10

euxassay_003172_11	euxassay_003172_12	euxassay_003172_13	euxassay_003172_14	euxassay_003172_15

euxassay_003172_16	euxassay_003172_17	euxassay_003172_18	euxassay_003172_19	euxassay_003172_20

euxassay_003172_21	euxassay_003172_22	euxassay_003172_23	euxassay_003172_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

hindgut

weak

regional

weak expression: see section 15

foregut-midgut junction

weak

regional

weak expression: see section 15 16

stomach

weak

regional

weak expression: see section 06 07 08 09

rectum

weak

regional

weak expression: see section 15 16

urethra of male

weak

regional

weak expression: see section 15 16

testis

strong

regional

strong expression: see section 07 08 09 10 21 22 moderate expression: see section 20

midgut

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17 18

bladder

strong

regional

strong expression: see section 14 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4188
Entity Detected:	Liph, lipase, member H ( MGI:2388029)
Sequence:	sense strand is shown >T4188 CCACGCGTCCGCGGGAACGCGGGAACGCCAGGCTCTGTGCGGAGTGCGACACGTCCTCGTGTTTGGGGCA CATGATCTCTTGAGGTTCCCTATGCTGAGGCTATGTTTTTTCATCAGTTTCATGTGCTTGGTGAAATCAG ACACGGATGAAACATGCCCGTCCTTCACCAGACTGAGCTTCCACAGCGCGGTGGTTGGTACAGGACTCTC CGTGAGACTGATGCTCTACACACAGAGAGACCAGACCTGTGCACAGATCATCAACTCCACAGCTCTGGGG AGCTTAAATGTGACCAAGAAAACCACTTTTATAATCCACGGTTTCCGGCCAACAGGCTCCCCTCCGGTTT GGATAGAGGAGCTGGTACAGAGTTTGATCAGTGTACAAGAGATGAATGTAGTGGTTGTTGATTGGAATCG AGGAGCGACAACTGTGATATACCCCCATGCGTCTAGCAAGACCAGACAAGTAGCTTCTATTTTGAAGGAA TTTATTGACCAGATGTTGGTCAAAGGAGCTTCTCTGGACAACATTTACATGATTGGAGTAAGTCTAGGAG CCCACATAGCTGGATTTGTTGGCGAGTCGTATGAGGGGAAGCTGGGGAGAGTCACAGGTCTTGACCCTGC AGGCCCTTTATTCAACGGGAGACCTCCTGAGGAGAGATTAGACCCCAGCGACGCACTGTTCGTGGACGTC ATCCACTCTGACACTGACGGTATAAAGTACTTCAAGTGTGACCACCAGATGTCCGTCTACCTGTACCTTG CATCCCTGCAAAATAACTGCTCCATCACGGCCTATCCCTGCGACTCCTATCGGGATTATAGGAATGGCAA GTGTGTCAGCTGTGGCGCTGGACAAATTGTGCCATGTCCCCGTGTAGGCTACTATGCTGACAGCTGGAAA GAGTACTTATGGGACAGAGATCCTCCGATGACGAAGGCGTTCTTCGACACAGCTGAGACAAAGCCATACT GCATGTATCATTATTTTGTGGACATTGTATCTTGGAACAAGAGCGTAAGAAGAGGGTTTATTACAATCAA ACTGAGAGGCGAAGATGGCAATATCACAGAATCCAAAATCGATCATGAGCCATCTGCATTTGAGAAATAC CATCAAGTGAGTCTCCTCGCAAGATTTAATCGAGATCTGGATAAGGTGGCAGAGATTTCCTTGTTGTTCT CCACAGGGTCTGTAGTGGGCCCAAAGTACAAGCTCAGGGTCCTGCAGATGAAGCTGAGGTCTCTGGCCCA TCCAGACAGGCCTCACTTGTGTCGATATGATCTCGTCCTTATGGAGAATGTTGAGACATCCTTCCAACCT ATCCTGTGCTCTCAGCAGCAGATGTAGCTGCTGTTAAGACCTGTGGGCAGTGATGACATCAAGATCTCTA CAGCTATAGGCTTTCAGTGCCTCACCCTATAGCGTCCTGAAGGCTTCCAAAGCATGGGGAAGAAAACCAA GGTTATTTTCGGCTGTGTTCCAAGGATGTCGCGTCACAAAAATGTCAAAGGACCTGCGATTATGAAACAC CACTGGGAGGGGGGGGGCGCCCTAACTAGGGCAAGACAGAAATAGCCCAGCTCCCTACAGCAAAGCACTT TGTCTGGCTGCGTCCCGAGGCCCCACTTGTTCCCCACTGTCGAATCTGCTGCCTGTCATACCACACCCTC AGGGCCGTGGGGCAAGCATCTAAATGGTCCCTGTGCCCAATCCTCTGCCATCTCGGCGGGAACCGAATGG AGAATGAACCAGACTACCTCACAGGGACTGTGGGTGGGGAGGGCCAGGCTGTGAAGGGTTTGGAAATCCC TTGGGTCCCTTGGGTGGACTACTGTGAACATGCTTTTCTTTTGATAGGGTTTGGCTCTTACGTAAAGACA CACAATCTCTCTTTTTCAACACCAGCTTTCCTCCTGGACTGTTTGCCTCGGGGCGACAAGAAGGCTCTGG CTTAGTCCAGACTCTTCTGAAGTGATGCTGATGGCCAGAGGTATTGGGCTGGGCTCATAGCCCTGAGGCC TGTGCAGCTAAGTGGAAGAAAGAGAAAAAAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4487939 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4487939 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003172 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source