EMAGE mouse gene expression spatial database

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EMAGE:30917

Wasf3 WAS protein family, member 3 ( MGI:2658986)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003179_01	euxassay_003179_02	euxassay_003179_03	euxassay_003179_04	euxassay_003179_05

euxassay_003179_06	euxassay_003179_07	euxassay_003179_08	euxassay_003179_09	euxassay_003179_10

euxassay_003179_11	euxassay_003179_12	euxassay_003179_13	euxassay_003179_14	euxassay_003179_15

euxassay_003179_16	euxassay_003179_17	euxassay_003179_18	euxassay_003179_19	euxassay_003179_20

euxassay_003179_21	euxassay_003179_22	euxassay_003179_23	euxassay_003179_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 20

cervico-thoracic ganglion

weak

regional

weak expression: see section 11 17

forebrain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

spinal cord

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 08 19 20

thoracic ganglion

weak

regional

weak expression: see section 14 15

dorsal root ganglion

weak

regional

weak expression: see section 09 10 11 12 17 18 19

facial vii ganglion

weak

regional

weak expression: see section 05 06 21 22 23

midbrain

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 09

trigeminal v ganglion

moderate

regional

moderate expression: see section 09 weak expression: see section 04 05 06 07 08 19 20 21 22 23

adrenal gland

moderate

regional

moderate expression: see section 09 10 18 19 20

cervical ganglion

weak

regional

weak expression: see section 10 18

hindbrain

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5371
Entity Detected:	Wasf3, WAS protein family, member 3 ( MGI:2658986)
Sequence:	sense strand is shown >T5371 GCCCTGTGTTACCAGGGGCCTCAAGATCCAGGTGAAGGGTAAGCTACAGGTGGGGATCCGAGGGGTCTGC AGATAGAGGAGGTACCCAGGCCCCTTGGTTACCCACCTTGGAGGAGGAGGCCTGAGGATGCGCCGCCTTC TCCGCAACGCCCCGCCCCCGCAGCGCCTACGCTGCCCTCTCCCGTTGGCTCTCCATTGGCCGCAGAGCTC GCCAATCCAGCCCCCTGGCCGTGAAGGGGGCGTGGCCGCGCGGTGCCGGGCCGGGGGCCGCGTCTGTCGC CGCTGCGCGCTGCGGTGCGGGCGGCAGGACGATGAAGCCGGGAGCGCGGCGCGCTCTGATCCCGCTTGCT GTTCCTACTGACACCTGAGCCGCGGTGGCGATGCTGCTGAGGCCCTTCCAGCCCACGAGTCAACCTACTT GATTGTCAGCCATGCCTTTAGTGAAGAGAAACATCGAACCCCGGCACCTGTGCCGGGGCGCCCTGCCGGA AGGAGTCACCAGTGAGCTGGAATGCGTGACCAACAGCACGCTCGCTGCAATCATCCGCCAGCTGAGCAGT CTGAGTAAACATGCAGAAGACATATTTGGTGAGTTATTTAATGAGGCTAACAACTTCTACATCAGAGCAA ATTCCCTTCAAGACAGAATCGATCGGCTTGCTGTCAAAGTCACCCAGCTGGACTCCACGGTGGAGGAGGT CTCACTACAGGATATCAACATGAAGAAAGCATTCAAAAGCTCTACCATCCAAGACCAGCAGGTGGTCTCC AAGAACAGCATTCCCAACCCTGTTGCTGACATTTATAATCAGAGTGACAAACCCCCGCCGCTGAGCATTC TGACACCGTACAGAGATGACAAGAAGGATGGGCTGAAGTTCTACACGGATCCCTCTTACTTCTTTGACCT CTGGAAAGAAAAGATGCTGCAGGACACAGAAGACAAGAGGAAAGAGAAACGACGTCAGAAGGAACAAAAG CGTGTAGATGGCACCACCCGAGAGGTGAAAAAGGTTAGAAAAGCCAGAAACAGGCGCCAGGAGTGGAATA TGATGGCGTATGACAAGGAGCTTAGGCCCGACAACAGGTTGTCTCAGAGTGTGCACCACGGAGCATCCTC CGAGGGATCTCTGTCCCCAGATACTAGGTCTCACACATCCGATGTCACCGATTATTCTTATCCTGCTACT CCCAACCATGCTTTGCAAGCACAGCCGGCGACACCCTCCTACACAGCTGGCGATGCACCACTGCATGGGA CCACAAACCAAGGCGCTGAGCACGAGTACCGACCGTCGTCTGCCTCGGCAAGGCACATGGCCCTGAACAG ACCTCAGCAGCCACCGCCACCGCCTCCTCCCCAGGCGCCGGAGGGGTCCCAGGCCTCTACGTCAGTGGCT CCAGCCGACTATGGGATGCTTCCAGCACAGATAATTGAATACTACAGCCCCTCAGGACCGCCACCACCTC CCCCTCCACCCATGATTCCCTCAGCACAAACTGCCTTCGTCAGCCCTCTCCAGATGCCCACACAGCCCCC GTTCCCTGCCTCAGCTGTCTCCACATACCCCACTCCTCCTCACCAGCCCTCCACTGGGCTCCTGGCCACA GCACCACCACCCCCAGGTCCCCCACCTCCTCCACCTGGTCCTCCTGGCCCCTCCTCCCTATCATCGTCCC CAATGCATGGTCCCCCTGTGGCTGAGGCAAAGCGCCCAGAGCCTGCACAGCCACCCATAAGTGATGCGAG AAGTGACCTCTTGGCTGCCATCCGGATGGGAATCCAACTGAAAAAGGTGCAGGAGCAACGCGAGCAGGAA GCCAAGCGGGAGCCTGTCGGGAATGACGTGGCCACCATCTTGTCTAGACGCATTGCTGTGGAGTACAGCG ACTCAGACGACGACTCTGAGTTTGATGAGAATGACTGGTCTGACTGAGGCACAAGGGCCAGGCGACCAGT GGACAGCCAGGGCCCCATATGCAAGTGTGTTAGGAGCTCCTGTGCTCTCTGCACCCTCACAGTGGTATTT CCCTGTAGCTTTAGTCCCTTTTGTATTAACAGTGTGATTTGCTTCTGCACATCCTTCTGGATTTGTTGAG TATTTACTGTGTAATACTTAAGTGCCACAGAACACAGCACACCGTGCTGCACACCAGGAAACTCGCAGTA TTAGTGCATCACCCTGGAACAGCATCTTGCTCCCCTCTCAGCCGTGAGAGTCCCCTCTCCTGTCCATCCT GATTTGGTGGGCTTGCCACGTATCTGCGCAGCTGCCCCACGTCAGCTCTCTGCAAGATCACTGGCTGTGT GTCTCAGTGATGTCTTCCAACCAGTGGGTGATACGTTTTCCTGTTTCTTCCCTTTGCAAATTGAATTCCA GGTAGATATGGTTGTAGAGGGGAAAGTTTAACAAATCAGAATGAAATGTTTGTCTGTTTGCACACTGGGC CTGGTGGCCTCCAAGGAAATGTGTACGATCGTTTGCTTTCTCTATCTTGGCGCTTGCAACCGAGAGCGGC CATTTTTATGAGGGGAGCCCTGTGGGTACTCATGTGGAAAGTGCCCACTGACTTTGGCAGATGGAGTTCA CTTTCATGGCTAGCCCTGATAGCGTTGGGCACCAGTTAGCCAGGCCATGTTCAAGCTGCCTAGAGTCTGC AGAGCTTGCATGGCAGAGCGGCCGC
Notes:	The probe template was PCR amplified from IMAGE:5363785 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5363785 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003179 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source