EMAGE mouse gene expression spatial database

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EMAGE:30932

Dst ( MGI:104627)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_016245_01	euxassay_016245_02	euxassay_016245_03	euxassay_016245_04	euxassay_016245_05

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euxassay_016245_11	euxassay_016245_12	euxassay_016245_13	euxassay_016245_14	euxassay_016245_15

euxassay_016245_16	euxassay_016245_17	euxassay_016245_18	euxassay_016245_19	euxassay_016245_20

euxassay_016245_21	euxassay_016245_22	euxassay_016245_23	euxassay_016245_24	euxassay_016245_25

euxassay_016245_26

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

esophagus

moderate

regional

moderate expression: see section 12 weak expression: see section 13

lower jaw molar

moderate

regional

moderate expression: see section 07 08 18 19

oral epithelium

moderate

regional

moderate expression: see section 06 07 08 16 17 18 19 weak expression: see section 05 10 11 12 15 20

upper jaw molar

moderate

regional

moderate expression: see section 06 07 18 19

vibrissa

weak

regional

weak expression: see section 04 20

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 04 11 12

lower jaw incisor

moderate

regional

moderate expression: see section 16 17 weak expression: see section 11 12 15

stomach

moderate

regional

moderate expression: see section 01 weak expression: see section 02 03 04 05

upper jaw incisor

weak

regional

weak expression: see section 11 12 15

urethra of male

weak

regional

weak expression: see section 12 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39390
Entity Detected:	Dst, ( MGI:104627)
Sequence:	sense strand is shown >T39390 TGGTCTGAAGACTGTCGAAGAAGTTCAGAGAAGTCTTAGCAAGTTTCTGACCAAAGCTACCTCCATTGCA GGACTTTATCTAGAATCTTCCAAAGAAAAAATGTCGTTCACTTCGGCGGCCCAGAAAATCATAATAGACA AAATGATAGCTCTAGCCTTTTTAGAAGCTCAGGCTGCAACAGGTTTTATAATTGATCCGGTTTCTGGTCA GACTTACTGTGTTGAAGATGCAGTTCTTCATGGCATCGTTGATCCTGAGTTCAGGAGCAGGCTCCTGGAG GCAGAGAAGGCAGTTTTGGGATATTCACATGCTTCTAAGACGCTGTCAGTGTTCCAGGCAATGGAAAATA GAATGCTTGATAGGAAGAAAGGTAAACATATCTTGGAGGCACAAATTGCCAGTGGGGGCGTCATTGACCC TGTGAGAGGCGTCCGTGTCCCTCCAGAAATGGCTGTGCAGCAGGGCTTGCTGAACAACGCCGTCCTACAG TTCCTGCATGAACCGTCCAGCAACACGAGAGTCTTTCCTAATCCCAACAACAAGCAGGCTCTGTATTATT CGGAGTTACTGCAGATCTGTGTGTTTGATGTAGATTGCCAGTGTTTTCTCTTGCCGTTTGGGGAGAGGGA AATATCCAATCTCAACATAGAGAAAACTCATAAAATTGCCGTGGTAGATACCAAAACTGGGGCGGAACTG ACGGCATTCGAGGCTTTCCAGAGAAACCTGATTGACAAGGGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 101029. Forward Primer - name:101029_F_cDNA_Dst, sequence:TGGTCTGAAGACTGTCGAAGAA; Reverse Primer - name:101029_N_SP6_cDNA_Dst, sequence:ACCCTTGTCAATCAGGTTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_016245 same experiment
	EMAGE:29262 same embryo
	EMAGE:29264 same embryo
	EMAGE:31323 same embryo
	EMAGE:29267 same embryo
	EMAGE:29248 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source