EMAGE mouse gene expression spatial database

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EMAGE:30934

Wdr31 WD repeat domain 31 ( MGI:1918604)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_003180_11	euxassay_003180_12	euxassay_003180_13	euxassay_003180_14	euxassay_003180_15

euxassay_003180_16	euxassay_003180_17	euxassay_003180_18	euxassay_003180_19	euxassay_003180_20

euxassay_003180_21	euxassay_003180_22	euxassay_003180_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 08 09 10 17

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 14 15 16 17

lateral ventricle choroid plexus

weak

regional

weak expression: see section 06 07 08 09 10 12 13 15 16 17 18

lung

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21

4th ventricle lateral recess

weak

regional

weak expression: see section 06 07 08 09 10 15 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5378
Entity Detected:	Wdr31, WD repeat domain 31 ( MGI:1918604)
Sequence:	sense strand is shown >T5378 CCACGCGTCCGGTGGAAGCTAAGGATGCCTGCTACCCGTGCTTTCGGGCCTCGGTGCTAGCCCACCGAGA TTAGGAAGTGGCTGTCTGACTTGTTTCCGAAGGCTGAATGAACTACAGATCATCAAATACAGCAGCTCAC CAAGAAGATCTGGTTGCTTTGGGGGGAACACCCCCTCAGCGCCTGTGAGGGATGTTGTTACTTGACGGCC AACTGAAACCAGCTCTTCCTCAGAAGGTCTTCTGTAGACTCTGGGCTGTGATGGGAAAACTGCAGAGCAA ACTCAGACACAGCACTTACTTATACAGCAGGCCTGATGGAATTATAGAAGAGAGAGTCCAGACTAAAGCT CCCCAAGAATACATCCCTGTTCACGTGGATACCGTCTCTGTGATTGCCACTCTGAACTCAGACCTTTGTA TCTCTGGAGGAAAAGACAAGACCGCCGTGGCCTACAATTGGAAAACTGGGCGTATGGTGAAAAGGTTCAC AGGGCACGAGCGGGAAATCACGAAGATAGCCTGTATTCCCAAAGCAAACCAGTTCTTCAGCGCCTCTCGA GACAAGACAGTCCTGATGTGGGATCTGCAGGGCTCCTCGCACCCTAGGCAGCAGCTGTCTGGCCATGCCA TGGTGGTCACTGGCTTGGCTGTGAGCCCAGACTCGTCCCAGCTGTGCACTGGCTCTCGGGACAATAGCCT GCTTCTGTGGGATGTGGGGACCGGACAGTGTGTGGAGAGAGCGTCTGTCTCCAGGAACCTGGTCACACAC CTGTGCTGGGTCCCGAGTGAGCCATACATAGTCCAGACCTCTGAGGATAAGACCATCAGATTATGGGACA GTCGGGGGCTTCAGATGGCTCACATGTTTCCCACAAAACAGCATATCCAGACCCATTGTGAAGTCAGCGT GGATGGACACACGTGTATTTCCTGCAGCAGTGGCTTTGGGGGTGAAGGCTGTGAAGCCACGTTGTGGGAC CTAAGACAGACTAGAGACAGAATGTGTGAGTATAAGGGGCATTTCCAGACAGTTGCATCCTGTGTCTTTC TACCGAAGTCCACGGCCTTGATGCCTATGATTGCTACCTCTGCACATGACTCCAAGGTGAAGATTTGGAA CCAGGATACTGGAGCTTGCCTCTCCACCTTGTCTCTGGATGGATCAGGACCCCTGACCTCACTGGCTGTT GGTGACATCTCCTCCTTGTTATGCGCAAGTTTCTACAGAGGAGTTCACTTACTCAGAGTGGACCACAGCC AAGGACTGGAACTGCAGGAAGTGGCAGCCTTCTAAAATCAAGAGATATTGATTGGATAATATCAAACCAT GGTCCCTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5364307 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5364307 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003180 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source