EMAGE mouse gene expression spatial database

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EMAGE:30935

Zscan12 zinc finger and SCAN domain containing 12 ( MGI:1099444)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003181_01	euxassay_003181_02	euxassay_003181_03	euxassay_003181_04	euxassay_003181_05

euxassay_003181_06	euxassay_003181_07	euxassay_003181_08	euxassay_003181_09	euxassay_003181_10

euxassay_003181_11	euxassay_003181_12	euxassay_003181_13	euxassay_003181_14	euxassay_003181_15

euxassay_003181_16	euxassay_003181_17	euxassay_003181_18	euxassay_003181_19	euxassay_003181_20

euxassay_003181_21	euxassay_003181_22	euxassay_003181_23	euxassay_003181_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 11 weak expression: see section 12 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5383
Entity Detected:	Zscan12, zinc finger and SCAN domain containing 12 ( MGI:1099444)
Sequence:	sense strand is shown >T5383 GGAGCGACGCGGTCCACTGGGTAAGAAGATTTACCAAGGAGTTATTACTTGAAAGATTGATACATCTTTT ATCTAGCTACGATGACATCTACTAGTGATACCAAGGTCTGTAAGAACCAGGGTGGACTTTTGGAAATAAA AATGGAGGAAGAATGTAAGTATACCACCAGACAAGATAGGAACCTACAAAAGAACACTTATAACAGAGAC GTCTTCCGAAAATACTTCAGACAGTTTTGCTACCAGGAAACTTCTGGACCCCGGGAGGCTCTGAGCCGGC TGCGCGAGCTCTGCAGACAGTGGCTGCGGCCGGACTTGAACAGCAAGGAGCAGATCCTGGAGCTGCTGGT GCTGGAGCAGTTCCTGACCATCCTGCCAGGGGAGCTGCAGGCCTGGGTGCAGGAGCAGAATCCGGAGAGT GTGGAGGAGGTGGTGACTGTGCTGGAGGATTTAGAGAGAGAGCTTGATGAACTAGGATACCGGGCCTCAG TCCAAACTGAAGAACAGGTAACGTTTCAGGAGGTGAAGCCTTTGGCAACAGAGCAGAAACCCAGCGTGTC CCTTCAGTTTGTCAAAGCCAAGCCTGGGTGTGAACTTGCAGGCCGTGAAGCCCAGGAGGAGCAAGTTTCA GGTGTTGAGACTGGCAATGAGCCCAGGAATGTCACTCTAAAGCAAGGCCTCTGGGAAGGGACGGAAGCAG AACAGAATCCTGCTAGCAGATTAGCAAAGGATGCACTTGAGTGTGAAGAAGCTCACAACCCTGGAGAAGA GTCTTCTGGTATTTCCCATGAAGACAGCCAGCCTCTGCGTAATGAAAATGGTGTAAATTCTCCTGCGAAC TCAGAGTACGCTAAACACCAGAGCATCTGTCCAGGGAGAAAAGTGCATGGGTGTGATGAGTGTGGAAAGA GTTTTACTCAGCACTCGCGCCTCATAGAGCACAAGAGAGTCCACACTGGAGACAGGCCCTACAAATGTGA AGTATGTGGGAAAACTTTCCGATGGAGGACTGTTCTTATTCGACACAAGGTGGTCCACACTGGAGAGAAA CCGTATAAATGTAATGAATGTGGCAGGGCTTTTGGCCAGTGGTCAGCACTTAACCAACATCAGAGACTTC ACTCGGGAGAAAAGCACTACCACTGTAACGAATGTGGCAAAGCCTTTTGCCAGAAAGCAGGCCTCTTTCA CCATCTCAAGAGCCATAGAAGAAACAGACCTTATCAATGTCTTCAGTGTAATAAAAGTTTTAATCGCCGT TCTACACTTTCTCAGCATCAAGGAGTTCACACTGGAGCAAAACCCTACGAATGCAACGATTGTGGGAAAG CTTTTGTTTATAACTCATCTCTTGCTACCCATCAGGAAACCCATCACAAGGAGAAACCCTTCACTCAAAG TGGTCCTATTCAGCAGCAGAGGAACCACACCAAGGAGAAGCCCTACAAATGCAGTGTATGTGGAAAAGCA TTTATTCAAAAAATAAGTCTTATAGAACACGAACAAATTCATACCGGAGAGAGACCGTATAAATGTGCTG AGGGCGGGAAGGCCTTTATTCAGATGTCAGAACTCACAGAACATTAGGGAACCCACACTGGGAAGGCCCT ACAAGTGTGATGAATGTGGCAAGACCTTCAGACAGGAGTCAGAGCTTGCTGAGCACTAGAGAATTCATTA TAGAAGTGGTCCCTAAAAGTGTAATGGGCGTGGGGAATTGTTCACGTAGAACTCAGCTCTTACTCATCAT CAGACTAGTCACAAAAGAGCAAGTACTCTGTGTGATGACTACAGGGAAGCTACCTCACGGTGTCGGCTGA TGAAGAGTATTGTATTTGTAATGCTTTGTGGTGACATTTCAGCCTTTGCTGTTACGAGACCATAAATATT TGATGAGCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5364568 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5364568 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003181 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source