EMAGE mouse gene expression spatial database

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EMAGE:30938

Gpd2 glycerol phosphate dehydrogenase 2, mitochondrial ( MGI:99778)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_003182_06	euxassay_003182_07	euxassay_003182_08	euxassay_003182_09	euxassay_003182_10

euxassay_003182_11	euxassay_003182_12	euxassay_003182_13	euxassay_003182_14	euxassay_003182_15

euxassay_003182_16	euxassay_003182_17	euxassay_003182_18	euxassay_003182_19	euxassay_003182_20

euxassay_003182_21	euxassay_003182_22	euxassay_003182_23	euxassay_003182_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 08 09 10 11 17 18 19 20 moderate expression: see section 21

nucleus pulposus

strong

regional

strong expression: see section 14 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4661
Entity Detected:	Gpd2, glycerol phosphate dehydrogenase 2, mitochondrial ( MGI:99778)
Sequence:	sense strand is shown >T4661 CCACGCGTCCGCCCGGGCCGCGGTGTCGCGGCGAAGGCGGTGGCTGAGTCGCCGGATCATCCCCCGGGGA GGCGGAGGCGCACGAGGGGAGGAGGAAGTGGCCGGGAGGGGCGTCGGCGGGCGGGTCGGGTTACCGTGGC CCCGCCTGACCCGGTGACATCCCGGCGCGCTCGCCGCGAGCTCAGGGCCACTCTGGTTCTCGGTGAGGCC GACTCCGTTCTGGCTGGAGGATCCTGACTTCCTTGCTCGCCGACCCCTTGCGCGTGACGACCGATCTCAG GCTGAGCAATGGCGTTTCAAAAGGCAGTGAAGGGGACTATTCTTGTGGGTGGAGGAGCTCTGGCCACTGT TTTGGGACTCTCTCAGTTTGCTCATTACAGAAGGAAGCAAGTGAGCCTGGCGTATGTGGAAGCAGCAGGA TACCTCACGGAGCCTGTGAACAGGGAACCTCCCTCCAGAGAAGCTCAGCTCATGACTTTGAAGAACACAC CCGAATTTGACATCCTTGTTATCGGAGGCGGAGCCACAGGGTGTGGCTGTGCACTAGATGCCGTCACCAG AGGACTGAAAACAGCCCTTGTAGAGAGAGATGACTTCTCATCGGGGACTAGCAGTAGAAGCACTAAATTG ATCCACGGTGGTGTGCGATACCTCCAGAAGGCTATCATGAACTTGGATGTTGAGCAGTATAGGATGGTGA AAGAAGCCCTTCACGAACGTGCCAACTTACTAGAAATCGCTCCTCATTTATCAGCTCCGTTGCCTATCAT GCTTCCACTTTACAAGTGGTGGCAGTTACCTTATTACTGGGTGGGAATCAAGATGTATGACCTGGTTGCA GGGAGTCAATGCCTGAAGAGCAGTTACGTCCTCAGCAAATCCCGAGCCCTGGAGCATTTTCCCATGCTCC AGAAGGACAAGCTGGTAGGCGCCATTGTCTACTATGACGGACAACACAACGATGCACGGATGAACCTCGC CATCGCCCTCACTGCTGCCAGGTACGGGGCTGCCACGGCCAATTACATGGAGGTGGTGAGCTTGCTCAAG AAGACAGACCCTGAAACCGGCAAAGAGCGAGTGAGCGGTGCGCGGTGCAAGGATGTGCTCACAGGGCAGG AATTTGACGTGAGAGCCAAATGCGTTATCAATGCCTCCGGCCCTTTCACAGACTCCGTGCGCAAAATGGA TGATAAAAACGTTGTTCCCATCTGCCAGCCCAGTGCCGGGGTCCATATTGTGATGCCCGGATACTACAGC CCTGAGAACATGGGACTTCTTGATCCTGCAACCAGTGATGGCAGAGTGATTTTCTTCTTGCCTTGGGAGA AGATGACAATTGCTGGCACCACTGATACGCCAACGGACGTCACGCACCATCCTATTCCTTCAGAAGAAGA CATTAACTTCATCCTGAATGAAGTGCGGAACTACCTGAGTTCTGACGTTGAAGTGAGAAGAGGGGATGTC TTGGCAGCCTGGAGTGGTATCCGTCCCCTTGTTACTGATCCCAAGTCTGCAGACACTCAGTCCATCTCTC GAAATCATGTTGTGGACATCAGTGACAGCGGACTCATCACAATAGCAGGTGGGAAGTGGACCACCTACCG CTCCATGGCAGAAGATACCGTGAATGCAGCTGTCAAGTTTCACAACTTGAATGCGGGACCGAGTAGGACT GTTGGGCTGTTCCTTCAAGGAGGCAAAGACTGGAGCCCCACACTCTACATCAGGCTTGTCCAGGATTATG GGCTTGAGAGCGAGGTTGCACAACATCTGGCCAAAACCTATGGTGACAAGGCTTTTGAGGTGGCCAAAAT GGCAAGTGTGACTGGAAAGCGGTGGCCTGTTGTTGGAGTGCGTCTTGTGTCAGAATTTCCATACATTGAA GCAGAGGTGAAATACGGCATTAAGGAGTATGCCTGCACTGCAGTTGACATGATCTCACGGCGCACCCGCC TGGCCTTTCTCAATGTTCAGGCTGCAGAGGAAGCCCTGCCTAGGATTGTTGAACTAATGGGAAGAGAGTT GAACTGGAGTGAATTGAGGAAACAGGAAGAACTTGAAACAGCCACGAGATTTCTGTACTATGAAATGGGC TACAAGTCTCGAACAGAACAACTTACAGATAGCACTGAAATCAGCCTGCTGCCTTCAGACATCGATAGGT ACAAGAAGAGATTTCACAAGTTTGATGAAGATGAAAAAGGCTTCATTACCATTGTTGATGTTCAGCGTGT CCTAGAGAGTATCAATGTACAAATGGACGAAAACACACTGCATGAAATTCTCTGCGAAGTAGATTTGAAC AAAAATGGACAGGTTGAGCTGCACGAGTTTCTGCAGCTGATGAGCGCAGTTCAGAAAGGAAGGGTCTCTG GAAGCCGACTTGCCATCCTGATGAAAACTGCCGAGGAGAACTTGGACCGCAGAGTTCCAATCCCCGTGGA CCGTAGTTGTGGAGGATTGTGAGTCTGACCAGTAAATCCGCCACCAGCAAGCATAGGACAGCCAGCGCTA TGTACAACCAGAGATGACTTAAACTCTAAAATAGTGGATCTCGTAGCTGCCTTTTTTTAAAACAAACAAA CAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5012142 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5012142 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003182 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source