EMAGE mouse gene expression spatial database

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EMAGE:30939

Ttc4 ( MGI:1919604)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002091_01	euxassay_002091_02	euxassay_002091_03	euxassay_002091_04	euxassay_002091_05

euxassay_002091_06	euxassay_002091_07	euxassay_002091_08	euxassay_002091_09	euxassay_002091_10

euxassay_002091_11	euxassay_002091_12	euxassay_002091_13	euxassay_002091_14	euxassay_002091_15

euxassay_002091_16	euxassay_002091_17	euxassay_002091_18	euxassay_002091_19	euxassay_002091_20

euxassay_002091_21	euxassay_002091_22	euxassay_002091_23	euxassay_002091_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 11 13 15 weak expression: see section 05 06 12 14

thymus primordium

moderate

homogeneous

moderate expression: see section 09 10 11 12 13

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 15 16 17 18 19 20 21

renal cortex

moderate

regional

moderate expression: see section 03 04 05 06 07 13 14 15 16

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 15 17

spinal cord

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4624
Entity Detected:	Ttc4, ( MGI:1919604)
Sequence:	sense strand is shown >T4624 CCACGCGTCCGGCCGAAGTTGCGAGCTGCCATGGAGAGTTCTGAGCCTGAGCCTACAGAGGACGCTTCCA TGGACGCGTTCCTGGAAAAGTTTCAGAGCCAGCCTTACCGCGGCGGCTTCCGCGAGGATCAGTGGGAGGA GGAATTTGACAAGATTCCCCTATTTATGAAAAAAGCGCCATCAGAAATTGACCCCGAGGAGTTTCCTGAC TTGGCCTGCCTCCAGTCGATGATTTTTGATGATGATCGATATCCAGAAGAGCAAGCCAAGACCTATAAAG ATGAGGGCAATGACTACTTTAAAGAAAAAGACTATAAGAAAGCTGTGCTTTCATACAGTGAAGGGTTGAA GAAGAAGTGTGCAGACCCTGATTTGAATGCTGTCCTGTATACCAACCGGGCAGCAGCACAGTACTATCTC GGCAATGTTCGTTCTTCCCTCAATGATGTGCTGGCTGCCAAGAAGCTAAAGCCCGGTCACCTGAAAGCCA TCATAAGAGGTGCCCTGTGCCATCTTGAACTGAAACACTTTGCTGAGGCTGTGAACTGGTGTGATGAGGG ACTACAAATTGATGCCAAAGAGAAGAAACTTCTAGAAATAAGGGCAAAAGCAGACAAGCTAAAGAGGATG GAAGAGAGGGATCTGAGGAAGGCAAAGTTGAAAGAAAAGAAGGAACAGCATCAGAATGAGGCTCTGCTCC AGGCCATCAAGGCTAGGAACATCAGGCTTGTCTCTGAATCAGCCGGTGAGGATGAAGATTCAGCCTCCAA TGGTCCAGCTGAGATACTCCTGGATGGGCTCAGCTCTGAGAACCCCTATGGAGCCAGGCTAAGTATAGAT GACCAGGGCAGGCTGAGCTGGCCTGTGCTGTTCCTGTACCCGGAGTACGCGCAATCAGACTTCATCTCTG CTTTTCATGAGGACACCAGGTTTATTGACCATCTAATGGCGATGTTTAGTGAAGCACCCTCCTGGGACTC AGAGCACAAGTACCACCCAGAGAATCTGGAGGTCTACTTCGAGGATGAGGACAGGGCGGAACTATACCAG GTGTCTCCCGATAGCACCCTGTTGCAGGTGCTGCAGCACCCCAGGTGCTGCGTGAAGGCCCTTACCCCGG CATTCCTGGTCTGTGTAGGATCCTCGCCTTTCTGTAGGAATTACCTCCAGGGGAAGAAGGTGCACAGGTA ACATGGGAGCCAGACCCTGGGGTCTCTTCCCTCAACTTCCTCTGCTGGACTTTGTACACGGGAATCATCT GGCCTAGAGTGTAGCACTCTATCCTCAGGGACAGTGGTTCCCAGCCTCTTGTTGGGGAAGAAGCTGCAGG ACCCCCTGAACCTAAGCCTGTCTGGCTGAGTGCTGGGCTCCAGCTGGGCCGCACTCTCCTCAGATTCACA GCCCTGCCCTGGCCAGTACATCTTTGGAGTCCATCTCTGAAAAAATCATCCACTGCAGTTACCAGAATAG CTGGCCTTGGTAACTCCCTAGCCCAGAGACTGTCGCAGGATGCAGTGACAGTCTTAACCTTTGTGGCATC GGGCACATACAGAAGGCACAACTCCAAGGCAATAAACCAGCCCTGCACATACTCTAAGGCCTTAAGGCTT GAAAGACTGGCTCAGTTGCTTCACCAGCCTGGAGGAAAGCTAAGATGCTGTGGATGCCATGAAGTGACTG CAGCCATGAAGCCACATCCTGTTCTGTTGACTGTATAAACTTGGATCCTGGGACTACTCAGAGCCCTACT GGATTCTCTACTTCTCAATGCCCATGCAAGGGCTTGTGCCACATCTGACAGAAGCTTGAGGAGTCAATGG GCAGTGGTTTGCAAGTAGGCTTGTGTTGTTTTCCCTTTATTGTGAGGTCTCATTTCTGAAATAGGAGTAG TCCTGGTGTCTGAGCAAAGCCCACAGCAGCTGGCCTAAAGCCATTGTTTGTTACATGAGTGGCAAATGTG TTTGTAGTAAACACTTGAGATTTGGGGGATTGTTCGTCAATATAGGAAAACTGACTAATACATTATTAAA CTGAAACCAGCTGGTGGCCTTCAGATGGGCTAATATGAGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5004463 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5004463 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002091 same experiment
	EMAGE:29250 same embryo
	EMAGE:31395 same embryo
	EMAGE:31393 same embryo
	EMAGE:31376 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source