Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30952

Apoh ( MGI:88058)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:30952 EMAGE:30952 EMAGE:30952 EMAGE:30952 EMAGE:30952
euxassay_002744_01 euxassay_002744_02 euxassay_002744_03 euxassay_002744_04 euxassay_002744_05
EMAGE:30952 EMAGE:30952 EMAGE:30952 EMAGE:30952 EMAGE:30952
euxassay_002744_06 euxassay_002744_07 euxassay_002744_08 euxassay_002744_09 euxassay_002744_10
EMAGE:30952 EMAGE:30952 EMAGE:30952 EMAGE:30952 EMAGE:30952
euxassay_002744_11 euxassay_002744_12 euxassay_002744_13 euxassay_002744_14 euxassay_002744_15
EMAGE:30952 EMAGE:30952 EMAGE:30952 EMAGE:30952
euxassay_002744_16 euxassay_002744_17 euxassay_002744_18 euxassay_002744_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
liver lobe
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T914
Entity Detected:Apoh, ( MGI:88058)
Sequence:sense strand is shown

>T914
CCTCGAGNCTGTTGGCCTACTGGACCACTTTGGTGAAGCTGGTGGGACGCATCCGCAATGGTTTCCCCGG
TGCTCGCCTTGTTCTCCGCCTTCCTCTGCCATGTTGCTATTGCAGGACGGATCTGTCCGAAGCCGGATGA
CCTACCATTTGCTACGGTTGTCCCCTTAAAGACATCCTACGACCCTGGGGAGCAGATTGTCTACTCCTGC
AAGCCAGGCTACGTGTCCAGGGGAGGGATGAGACGGTTTACCTGTCCTCTCACAGGAATGTGGCCCATCA
ACACCCTGAGATGTGTCCCCAGAGTATGTCCTTTCGCTGGAATCTTAGAAAATGGAATTGTACGCTACAC
GAGTTTTGAATATCCCAAGAACATCAGTTTTGCTTGTAACCCTGGGTTTTTTCTGAATGGGACCAGCTCA
TCTAAGTGCACGGAGGAAGGAAAATGGAGCCCAGATATTCCTGCTTGTGCTCGCATCACCTGCCCGCCAC
CACCAGTTCCAAAGTTTGCACTCCTTAAGGATTATAGGCCTTCAGCTGGGAACAAC
Notes:The probe template was PCR amplified from IMAGE:1924737 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1924737 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002744 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS