EMAGE mouse gene expression spatial database

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EMAGE:30959

Kcnab2 potassium voltage-gated channel, shaker-related subfamily, beta member 2 ( MGI:109239)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002988_01	euxassay_002988_02	euxassay_002988_03	euxassay_002988_04	euxassay_002988_05

euxassay_002988_06	euxassay_002988_07	euxassay_002988_08	euxassay_002988_09	euxassay_002988_10

euxassay_002988_11	euxassay_002988_12	euxassay_002988_13	euxassay_002988_14	euxassay_002988_15

euxassay_002988_16	euxassay_002988_17	euxassay_002988_18	euxassay_002988_19	euxassay_002988_20

euxassay_002988_21	euxassay_002988_22	euxassay_002988_23	euxassay_002988_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 16 17 18 19

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 20 21

thymus primordium

strong

regional

strong expression: see section 11 12 13 14 15 16

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 17 18 19 20 21 22

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 13 14 15 weak expression: see section 08 16

ventral grey horn

weak

regional

weak expression: see section 11 12 13 14 15 16

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4243
Entity Detected:	Kcnab2, potassium voltage-gated channel, shaker-related subfamily, beta member 2 ( MGI:109239)
Sequence:	sense strand is shown >T4243 GGGGGTGCTAAAGTCGCAAGGGCAGGGCAGGAAGGAAGATCCGGAACGGGGCGTGGGATCCGGAGCTGGA GGCCCCCAGGATTCCCCCTCTGTGAAAAGGCACGCTGATCTGTCTGGATAAGTGTGGCCGGCCCCATGTA TCCGGAATCAACCACGGGGTCCCCAGCTCGACTCTCCCTGCGGCAGACAGGCTCCCCCGGGATGATCTAC AGTACTCGTTATGGGAGTCCCAAAAGACAGCTCCAGTTTTACAGGAATCTGGGCAAATCTGGCCTTCGGG TCTCCTGCCTGGGGCTTGGAACATGGGTGACCTTCGGGGGCCAGATCACGGATGAGATGGCAGAGCACCT AATGACCTTGGCCTACGATAATGGCATCAACCTGTTCGATACGGCGGAGGTCTACGCTGCTGGAAAAGCT GAAGTGGTATTAGGGAACATCATTAAGAAGAAGGGATGGAGACGGTCCAGCCTTGTCATCACCACCAAGA TCTTCTGGGGTGGAAAAGCGGAGACTGAGAGAGGCCTTTCCAGGAAGCACATAATTGAAGGACTGAAAGC GTCCCTGGAGCGGCTGCAGCTGGAGTACGTGGATGTGGTTTTTGCCAACCGCCCAGACCCCAACACGCCC ATGGAAGAGACCGTGCGGGCCATGACCCATGTCATCAACCAGGGGATGGCCATGTACTGGGGCACATCAC GCTGGAGCTCCATGGAGATCATGGAGGCCTACTCGGTGGCTCGGCAGTTCAACCTGATCCCGCCCATCTG CGAGCAAGCGGAATATCACATGTTCCAGAGGGAGAAGGTGGAGGTCCAGCTGCCAGAGCTGTTCCACAAG ATAGGAGTAGGTGCCATGACCTGGTCCCCTCTGGCGTGCGGCATCGTCTCAGGGAAGTATGACAGCGGGA TCCCACCCTACTCCAGAGCCTCCCTGAAGGGCTACCAGTGGTTGAAGGACAAGATCCTGAGTGAGGAGGG TCGCCGCCAGCAGGCCAAGCTGAAGGAACTGCAGGCCATTGCCGAACGCCTGGGCTGCACCCTACCCCAG CTGGCCATAGCCTGGTGCCTGAGGAATGAGGGTGTCAGCTCCGTGCTTCTGGGTGCTTCCAATGCAGAAC AACTTATGGAGAACATTGGAGCAATACAGGTCCTTCCAAAATTGTCGTCTTCCATCGTCCACGAGATCGA CAGCATTCTGGGCAATAAACCCTACAGCAAAAAGGACTATAGATCCTAAGTCTACTCCCCACCCCTCGTG CCCCACCCGGACAGTTCCCGGTTCCCTCCTCGTCTCTGTTCGCTCGCTTCTGCTGTTCCAAAGCCAAATG CAGAGTGTGGTTCACATCCAAAGGGAAATCACTATGCCCAGACGTGACAGGGAATCGACCTCCAAAGTCA CCGCCAGATGCCGCCTGCCGCTTCTTCACTGGATACTGTCCGACAACGGATGCCAGGGGATGGCATCAGT TAGGGGGACATCCAGAGGCTCCCGCCCAAGCCCACCACCTCTGCTTATCCTTCAAGAAGAAACACTTGCC CCCAGCCATGGCCCTTCACGGAAATTCTAGAAGCCATGGCCGGGTGGAGGTCTCATCCTTAGAGCAGGAG GGGCCGCCAAGAGCCCATCTCAAAAGAGGGGTGTGCACCCTTCTCCCAGAGTCCTGGTGGTTAGAGCAGG GCCCTCCCTCTTTCCATGGACACCAGGTACTTTGCTCAAGGCTGGCCTGGATCCCATTCTTCCTAGGTCT TGACCTGCCCCACATACCCACCGAGCCTTGGCTGACATAGCTACCCAGCTGGCCCTTGGCCCCCACCCTC CCCCTTGCTGGCAGGGCCAGGGATTCCAGCTGGCTTTCCCAGCATCTTCCAACTGCTGAGCCCCACAGCA GGCAAGCTCAGCAGGCTTGGATGGAGCCCATCGGAGTATGCCTCTCAAGACAGACACTCGTGGGCAAGTG TGGCAGCTGGCCCCACCGCAGGCAGGTGGCAGCAGGCTTGCAAGACCATATTGCTCTCACAGACCTTCCA ATACCTCTGCTGTACCGGGGCAGTGGGCTCATGATGTGGGCTTTGCCCAGATGAAGCACTTAGAGCTATG TTGCAAGCCAGGGAACTACGGGAGCAGCTCCAGTAATAGTCTCCGTGTTTTAAGAATAGATCCCAGAGCT TCTCCCTGGAAAAATGGCTAGGACCACCCATTCCCTTCTGCGTACCCTCATGCTGGCCTAGGGCAGGATG GGGAGAGGAAGTAGTGGAAAGGATAGGCAGCCCTTTATCACTGGGATAAATCCTGCATGGATGTTGATGC CAGGGAGCCTGCGGGATTGGTCAGTACACACTGCCTGGAGGCCCCATCCCATGGTTTTGTGATGCTCTGG GGGACAGGGTCCTACCCAGCTGCCTCCCTAGGAGACCAGCCTCCTGCCCTGCTGCACAGACCATGGCCAG CTCCCAGGCACAAGTCCTAGATGCTTAGCACCCTAGCTTTGCCTTCTGTAGCTGCTATTACAAGGCTGCT CCTTGCTGCTCTTAAGGCTGGGCAGAGGTGAGGACAGGGGTGGGGGTTGGGGGGGTTTATGGGGAGGAGA CAGGAAGAAAGGCAGACAAACCCCCCCCCGAAAACCCTGAGCTCTCTAACCTTCGCTGCTCATAGTGCTG ACCCTAAGACCTCAGAAGCAGCTCTGTGTGTGGGGGGATGGAGACCATGGGCTCTGGTATTTCCATGCCT GGCTAGCAGAGCCAGCTTTCTGGACAGGGCCCTGCAGTGGCCCAGCAGGAAGTACCAGACCTGTCCACAC AGCTGGCCTTGGCCCTGCTTGTTAGGTGGGCCAGGCATGCAGGGATGCCCACAGTGGGAGCTACTGCCTG CCTGTGAACCACACCTCAGAGCTAAGCTCCTGCTGGTGATGTGCAAGAGACAGGAAAACAGGCTGCCGGG CTAAGGCCCATAGGATTTAGCCCACAGGAGCCCTAGATGCAAAAGACATGTTAACCAAGTGTTTCTGAGA GGTTGTCTTCCACACCCACTCTCGAAGCGTCAGGGATTTGCTTCTTAAACTTCTGCGGAGGGGTTTGGCT TGGGAGGCCATGTAGAGCAAGTACAGACAGCCTGTTAGTGGGAGCTTGCCCCCAGGCCCTCAAAGGGTGT AAGGCCAGCTCCTTCCTCCATGGCCTCTCCTTCCTTTAGCCCAGCTGCCTGCAGGCCAGCCCAAGCCAGC TTCTCTTCACCTTGAAGTAGTCTGCCAGGCCTGGTGCAGGAGGAGGGCCATGTAGAGAGAGGATCTGGCA CTCGGAGCCCAAGGTCCCAAGGCACAGCAGCGCACCTGTGTGCCTGCTCTCCTCATGTACATACATAACG TGCTTTCTCTCCCCGTTTTTTGTTTGTTTTTTTTTAAACGAGCGTGGCTCCTCGCTAACTGCACCCCACC AGGCCGACAGCAAACGCCTCCAGCTTCCCGACAGACTCAGACCAAGGTGCGGCCCCGTATTTATGGAATG GCAAATAAAACCCGAGCCCTTTGGTCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:4503373 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503373 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002988 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source