EMAGE mouse gene expression spatial database

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EMAGE:30982

Pola2 polymerase (DNA directed), alpha 2 ( MGI:99690)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002999_01	euxassay_002999_02	euxassay_002999_03	euxassay_002999_04	euxassay_002999_05

euxassay_002999_06	euxassay_002999_07	euxassay_002999_08	euxassay_002999_09	euxassay_002999_10

euxassay_002999_11	euxassay_002999_12	euxassay_002999_13	euxassay_002999_14	euxassay_002999_15

euxassay_002999_16	euxassay_002999_17	euxassay_002999_18	euxassay_002999_19	euxassay_002999_20

euxassay_002999_21	euxassay_002999_22	euxassay_002999_23	euxassay_002999_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 13 14 15 16 17

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23 24 moderate expression: see section 01 02

midbrain ventricular layer

strong

regional

strong expression: see section 10 11 12 14 15 16 moderate expression: see section 09 13 17 weak expression: see section 18

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 10 11 12 13 17 18 19

rest of cerebellum marginal layer

strong

regional

strong expression: see section 08 09 18 19 20 21 moderate expression: see section 04 05 06 07

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 08 09 18 19 20 21 moderate expression: see section 05 06 07

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4655
Entity Detected:	Pola2, polymerase (DNA directed), alpha 2 ( MGI:99690)
Sequence:	sense strand is shown >T4655 CCACGCGTCCGGTTTGGCGGGTTTTTCCTGTGCTGTGCTCCTCACGGAGAAGCTAAGGTGTAGTCCTCGG AAGCAAGTTTTCTCTCTGGATCGAGACCTCTCGAGGGCTTTTGAAGTATTTCTCTGTGACCTGGTGGCTG GGGTCGTTTGAGGGCTTAGGCCAGGTGGTCTCGTGACACGTCCCTGTCACCTTCCGGTAAAGCGAAAGGA TAAGCGTCTGCAGGGTAGAGCTGAGGTCGGGGCCCGGCGGCAGGCGCCCGGCCACTCCCGGGTCACCATG GCCGTGTCCACCCAGCAGCTGGCGGAGGAACTACAGATCTTCGGCCTGGACTACGAGGACTCCCTGTTGG AGAAACTGGCAGAGCTGTGTGTTCTATACCGACAGACTGAGGACGGGATGGTCAGTGAGCTCATCGCCTT CTGCACCAGTGCGGGGAAGACATGCCTTACTGTAGACATCCTGAACTCTTTTGAATACGAGGTTCTGAAC AAAAAACTCTCCAAAGCCTGGCACAGTGCCTCCAAAGACAGTGGGCATGCAGGAACCAGAGACATTGTTT CTATACAAGAGCTAATTGAAGCAGAGGAGGAAGAGGAGACCCTCCTAAGCTCTTATACCACACCCTCTAA GGGTCCTCTCAAGCGAGTTAGCAGCACCCCAGAAACCCCCCTAACCAAAAGGAGCGTCGCTGCTCGCAGC CCACGTCAGCTTCTCTCACCGTCAAGTTTCTCCCCGAGTGCTACCCCCTCCCAGAAATACACCTCCAGAA CCAACCGGGGAGAAGTGGTGACCACCTTTGGCTCCGCACAGGGCTTGTCTTGGTCCGGGAGAGGAGGCTC TGGAAGCGTCAGTCTAAAGGTCGTGGGGGACCCAGAGCCACTCACTGGCAGCTACAAAGCGATGTTTCAG CAGCTCATGGGTGTTCGAGAAGTTCTGACCAGTAAGATCGAAGAGCTCGGCAGCGAGCTGAAGGAGCATC ACAAGATCGAGGCCTTTACCCCTCTCCTGGTCCCAGCGCAAGAGCCTGTTATTCTCCTGGGTCAGATTGG CTGCGACAGCAACGGGAAGCTGAACAGCAAGTCCGTGATTCTCGAGGGGGACCAGGAGCACTCCTATGGC GCGCAGATCCCAGTGGACCTATCGGAACTCAAAGAGTATTCCCTCTTCCCTGGACAGGTTGTAATTATGG AAGGATTCAACACCACTGGTAGGAGACTTACTGCCACCAAACTCTATGAGGGCGTGCCACTTCCGTTCTA TCAGCCTACTGAAGAGGAGGGAGCTTCTGAGCAGACTATGGTGGTAGTTGCCTGCGGGCCTTATACCACC TCCGACAGCATCACCTATGACCCCCTGCTTGATCTGATCGCCATCATCAACCGTGACCAGCCAGATGTCT GCATCCTGTTCGGACCTTTCCTGGATGCTAAACATGAACAGGTGGAGAACTGTAAGTTGACAAGCCCATT CGAAGACGTTTTCAAGCAATGCCTGAGAACAGTTATCGAAGGAACGAGAAGCTCCGGCTCCCACCTTGTC TTTGTCCCGTCTCTGAGGGATGTCCATCATGAGCCAGTATACCCACAGCCGCCTTTCACCTTCTCCGAGC TGTCTCGAGAGGACAAAAAGCGAGTGCAGTTTGTGTCAGAGCCCTGCAGCCTCTCCATAAATGGAGTGAT GTTTGGTTTGACATCCACGGACCTGCTGTTCCACATCGGGGCTGAGGAGATCTTTAGCTCTTCCGGGACG TCAGACAGATTCAGCCGAGTCCTTAAGCATATCCTGACGCAGAGAAGTTACTACCCACTTTATCCACCCC ACGAGGACATGGCCATAGACTACGAGAACTTCTATACCTATGCCCAGCTGCCGGTCACCCCCGATGTCTT CATAGTGCCGTCCGAGCTGAGATACTTTGTGAAGGACATCTTTGGCTGTGTCTGTGTGAATCCTGGGCGG CTCACGAAAGGACAAGTGGGCGGCACCTTTGGCCGCCTCTACCTCAGGAGGCAGCCCAAGGCCATGGACG GTGGGGGCAGGCAGGGCCTGAGCGTGGCAGCGCAGGTGGTCAGAATCTGAGTCCTCAGCCTGGTTGCCTG GCCTCACAAACACCAAGAGCCCAGGTATGGCTCTCTGGTGGCCAAACTGTTGGAGTACCTGACAAGGATC TGGCCACAGAGACAGGACTGTAGGGCTGGGCCGTCCAGTCACGGCTTCCCACTGTCCCTCACCCCCATGC AGCGTGGCTCTGATCTGAATTACGCCCTGCTTCTCTCCTGCTTCATGAGGTAGATGTACAGAAGTGAGCC ATCTATGGACACAAAGGCAGGAAAACTGACTTTTTTTTGTATGAATCAAATACAGCGTGTGGAGAAATAA AAATTTTTTTAGTATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5011204 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5011204 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002999 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source