EMAGE mouse gene expression spatial database

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EMAGE:30985

Pip5k1a phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha ( MGI:107929)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002998_01	euxassay_002998_02	euxassay_002998_03	euxassay_002998_04	euxassay_002998_05

euxassay_002998_06	euxassay_002998_07	euxassay_002998_08	euxassay_002998_09	euxassay_002998_10

euxassay_002998_11	euxassay_002998_12	euxassay_002998_13	euxassay_002998_14	euxassay_002998_15

euxassay_002998_16	euxassay_002998_17	euxassay_002998_18	euxassay_002998_19	euxassay_002998_20

euxassay_002998_21	euxassay_002998_22	euxassay_002998_23	euxassay_002998_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 07 08 09 10 21 22 23 24

lower jaw incisor

moderate

regional

moderate expression: see section 13 14 17 18

upper jaw incisor

moderate

regional

moderate expression: see section 13 14 17 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4259
Entity Detected:	Pip5k1a, phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha ( MGI:107929)
Sequence:	sense strand is shown >T4259 CCACCCTGAGCCGAGCGGTGGAGTTGGGGATAAACAGGCAGTGGCTGGGGAGGTGAGGGAGGGGGGGCAT TCGCTTTTGGGGGCACCTCAGGTGCCTAGGTTTTCCTTTAGGTTTGTTCTATCCGAGACGCGGGACTCCC GGGGCCCGGCTCAAGTGTTCCTGTCTGGTAAGAGAAGGTAGCTTTGGCGGAGAGCAGTTCAAGTTGCTAG GAAGGTGCGAGACTGCAGCTCTCCCCCATGCCGGGCAAGTGGTGCGGTCCTGAGCTTGTCCTGGAGCCTG AACAACGCCTGTGAGAAGCCCGCCGAACGCGGGTTCTGTGAAGAGACGTGGGGAAGATTCGATTCCGAGA AAAGGAAAAGCCGGATTGAAAGGGAGCGAAGCCGCTGAGGGGGAGGGGGCTGCCAAGATGGCGTCCGCCT CCTCAGGGCCAGCGGCGGCCGGGTTTTCATCCCTTGATGCCGGGGCCCCTGCTGGTACCGCAGCAGCATC TGGAATCAAGAGAGCCACAGTATCTGAGGGACCTTCCGCCTCTGTCATGCCTGTTAAAAAAATAGGCCAT CGAAGTGTTGATTCCTCTGGAGAGACTACATACAAGAAGACAACTTCATCAGCCTTGAAAGGTGCCATCC AGTTAGGCATCACTCACACTGTGGGCAGCCTGAGCACCAAACCAGAGCGTGATGTCCTCATGCAAGACTT CTACGTGGTGGAGAGCATCTTTTTCCCCAGTGAAGGCAGCAACCTGACACCGGCTCACCATTACAATGAC TTTCGATTCAAGACCTATGCGCCTGTTGCCTTCCGTTACTTCAGAGAGCTCTTTGGCATCCGGCCTGATG ATTACTTGTACTCCCTTTGCAGTGAGCCATTGATTGAACTCTCCAATTCTGGAGCTAGTGGTTCCCTCTT CTATGTGTCCAGTGATGATGAATTCATCATTAAGACCGTCCAGCATAAAGAAGCAGAATTTCTGCAGAAG TTGCTTCCAGGATACTACATGAATCTTAACCAAAACCCTCGTACTTTGCTGCCCAAATTTTATGGATTGT ACTGTGTGCAAGCAGGCGGCAAGAACATACGAATTGTGGTGATGAACAATCTCTTGCCTCGGTCAGTCAA AATGCATATGAAATATGACCTGAAGGGTTCAACTTACAAGCGGCGGGCTTCTCAGAAAGAACGAGAGAAA ACTCTCCCCACCTTTAAAGACCTGGACTTCCTACAAGATATCCCTGACGGCCTATTTTTGGATGCTGACA TGTACAGTGCTCTGTGTAAGACTCTACAGCGTGACTGTTTGGTGCTGCAGAGCTTCAAGATAATGGACTA CAGCCTGTTGATGTCCATCCACAACATGGACCATGCACAGAGGGAGCCCACGAGCAATGACACACAGTAC TCGGCTGACACGCGCAGACCAGCCCCACAGAAGGCGCTCTATTCCACAGCTATGGAATCCATCCAGGGCG AGGCTCGACGGGGCGGCACTGTGGAGACTGAGGACCACATGGGTGGCATCCCTGCCCGGAATAACAAAGG AGAAAGGCTCCTGCTTTATATTGGCATCATTGATATTCTTCAGTCCTACAGGTTTGTTAAGAAGTTGGAG CACTCTTGGAAAGCACTGGTCCATGATGGGGATACGGTGTCAGTGCATCGTCCAGGCTTCTATGCTGAGC GGTTCCAGCGTTTCATGTGCAACACAGTGTTCAAGAAGATTCCCTTGAAGCCTTCTCCTACCAAAAAGTT TCGGTCTGGCCCGTCTTTCTCTCGGCGATCAGGCCCCAGCGGCAACTCCTGCACCTCCCAGCTGATGGCC TCTGGGGAACACAGAGCACAAGTGACCACCAAGGCGGAAGTGGAGCCAGATGTACACCTTGGGCGTCCTG ATGTCTTACCTCAGACTCCACCTTTGGAGGAAATAAGTGAGGGTTCACCTGTTCCTGGCCCCAGTTTCTC ACCTGTAGTTGGACAACCTTTGCAAATACTAAATTTGAGTTCAACCTTGGAAAAGCTTGATGTTGCAGAG TCAGAGTTCACCCACTGAGCAGTGAGCCTCAGAAGACCTGAAACAAGACTCTGCTGTCGCTTTGATCCCA AGATGTCAGCCCTTGCCCCAGGAATGCTGAATCGTCTTCTTGGTCATCAACAGAGGAGAGAATGTGGAGC TAGCAGGGCTCTGACACTGTCTCCCCTTCCTCTGGGCAGTAGTGCCTTTGACAGTTGAGGACAGCATCGT CCCCACCACTCCAGAAGTGGGTGGCGTGAATTTTCCACTGCCTGCCATGGCTTCTATAGCTGAATATTTT TCAAACCCCCATTCTTTGTGCTGACATGGGGTTGCTGGACTGGTGGTTTCCTCCTGTTTACCTTTCTCCT GGAGCTGGACTCTTCATTTCCTCAGGACGGACTGGCTGGACATTGTTCCTGCCTTGGCTCTCCCGCTGGT CTTTGGAGACTCCCAATGATTTGTATAAAGGTGTTTGGGGGAAAGTAAGGATCATCCTTACTCCCAACCT TCTTTTCCTTAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4503697 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503697 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002998 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source