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EMAGE:30991

Etv6 ets variant gene 6 (TEL oncogene) ( MGI:109336)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
lower jaw molar
strong strong
regionalstrong expression: see section 04 05 16 17
submandibular gland primordium
strong strong
regionalstrong expression: see section 04 05 06 07 13 14 15 16
upper jaw molar
strong strong
regionalstrong expression: see section 04 05 17
thymus primordium
strong strong
regionalstrong expression: see section 12 moderate expression: see section 08 09 10 11
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 06 07 09 10 11 13 14 15 16
parotid gland
strong strong
regionalstrong expression: see section 01 19
lower jaw incisor
strong strong
regionalstrong expression: see section 12 moderate expression: see section 08 11
upper jaw incisor
strong strong
regionalstrong expression: see section 12 moderate expression: see section 08 11
thyroid gland
moderate moderate
regionalmoderate expression: see section 08 09 12 13
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30443
Entity Detected:Etv6, ets variant gene 6 (TEL oncogene) ( MGI:109336)
Sequence:sense strand is shown

>T30443
AGTGGCTCAAGTGGGCAGAAAATGAGTTTTCTTTAAGGCCCATTGAGAGCAACAAGTTCGAAATGAATGG
CAAGGCCCTCCTGCTGCTGACCAAAGAGGATTTCCGCTACCGATCTCCTCATTCAGGCGACGTGCTCTAT
GAACTCCTTCAGCATATCCTGAAGCAGAGGAAATCTCGAATGCTCTTCTCACCATTCTTCCCCCCTGGGG
ACTCTATCCACACCAAGCCAGAGGTCCTCCTGCATCAGAACCATGACGAAGATAACTGTGTCCAGAGGAC
ACCCAGGACGCCCGCGGAGAGCGTGCACCACAACCCTCCCACCATCGAACTCTTACATCGCCCTAGGTCA
CCCATCACCACAAACCACAGGCCTTCTCCTGACCCCGAACAGCAGCGGCCCCAGCGGTCCCCCCTAGACA
ACATGAGCCGCCGCCTCTCGCCAGTGGAGAAAGCCCAGGGGCCCAGGCTACAGCAGGAGAACAACCACCA
GGAAACGTACCCCCTGTCAGTGTCTCCTGTCGAGAATAATCACTGCCTGCCCTCAAGCCCCTGGCAGGAG
AGCACTCGAGTGATCCAGCTGATGCCCAGCCCCATCATGCACCCTTTGATCCTGAACCCCCGGCACTCGC
ACTCGGTGGACTTCAAACAGTCCCGGCACTCCGAGGATGGGATGAATCGGGAAGGGAAGCCCATCAACCT
GTCTCATCGGGAGGACCTGGCTTACTTGAACCACATCATGGTCTCTATGTCCCCACCGGAAGAGCACGCC
ATGCCCATTGGGAGAATAGCAGACTGTAGACTGCTTTGGGATTATGTCTATCAGTTGCTGTCTGACAGCC
GGTACGAAAACTTCATCCGATGGGAGGACAAAGAATCCAAAATATTCCGGATAGTGGATCCCAACGGACT
GGCTCGAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6513848), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58561. Forward Primer - name:058561_F_IRAV105_f04_Etv6, sequence:AGTGGCTCAAGTGGGCAG; Reverse Primer - name:058561_R_SP6_IRAV105_f04_Etv6, sequence:AGTCGAGCCAGTCCGTT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_012303 same experiment
 EMAGE:32066 same embryo
 EMAGE:31449 same embryo
 EMAGE:29298 same embryo
 EMAGE:29287 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS