EMAGE mouse gene expression spatial database

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EMAGE:30993

Dlk1 delta-like 1 homolog (Drosophila) ( MGI:94900)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_012302_01	euxassay_012302_02	euxassay_012302_03	euxassay_012302_04	euxassay_012302_05

euxassay_012302_06	euxassay_012302_07	euxassay_012302_08	euxassay_012302_09	euxassay_012302_10

euxassay_012302_11	euxassay_012302_12	euxassay_012302_13	euxassay_012302_14	euxassay_012302_15

euxassay_012302_16	euxassay_012302_17	euxassay_012302_18	euxassay_012302_19	euxassay_012302_20

euxassay_012302_21	euxassay_012302_22	euxassay_012302_23	euxassay_012302_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

ventral grey horn

strong

regional

strong expression: see section 10 11 12 13 14

pituitary gland

strong

regional

strong expression: see section 10 11 12 13 14 15 16

mesenchyme

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 24

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 13

axial skeleton

strong

regional

strong expression: see section 11 12 13 14 15 16

axial skeleton tail region

strong

regional

strong expression: see section 12 13 14

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 08 09 10 15 16 17 18

pons mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 14 15 16 17 18 19

spinal cord ventricular layer

strong

regional

strong expression: see section 13 14 15 16

hypothalamus mantle layer

strong

regional

strong expression: see section 09 10 11 12 14 15 16 17 18

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17

telencephalon ventricular layer

strong

regional

strong expression: see section 13

midbrain ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15

hypothalamus ventricular layer

strong

regional

strong expression: see section 12 13 14 15

telencephalon mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 12 14 15 17 19 20 21 moderate expression: see section 22

midbrain mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 09 10 11 12 14 15 16 17 18

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30444
Entity Detected:	Dlk1, delta-like 1 homolog (Drosophila) ( MGI:94900)
Sequence:	sense strand is shown >T30444 GCATCTGCAAGGATGGCTGGGACGGGAAATTCTGCGAAATAGACGTTCGGGCTTGCACCTCAACCCCCTG CGCCAACAATGGAACTTGCGTGGACCTGGAGAAAGGCCAGTACGAATGCTCCTGCACACCTGGGTTCTCT GGAAAGGACTGCCAGCACAAGGCTGGGCCCTGCGTGATCAATGGTTCTCCCTGCCAGCACGGAGGCGCCT GCGTGGATGATGAGGGCCAGGCCTCGCATGCTTCCTGCCTGTGCCCCCCTGGCTTCTCAGGCAACTTCTG TGAGATCGTAGCCGCAACCAACAGCTGTACCCCTAACCCATGCGAGAACGATGGCGTCTGCACCGACATC GGGGGTGACTTCCGTTGCCGCTGCCCAGCTGGATTCGTCGACAAGACCTGCAGCCGCCCGGTGAGCAACT GCGCCAGTGGCCCGTGCCAGAACGGGGGCACCTGCCTCCAGCACACCCAGGTGAGCTTCGAGTGTCTGTG CAAGCCCCCGTTCATGGGTCCCACGTGCGCGAAGAAGCGCGGGGCTAGCCCCGTGCAGGTCACCCACCTG CCCAGCGGCTATGGGCTCACCTACCGCCTGACCCCCGGGGTGCACGAGCTGCCTGTTCAGCAGCCCGAGC AACACATCCTGAAGGTGTCCATGAAAGAGCTCAACAAGAGTACCCCTCTCCTCACCGAGGGACAGGCCAT CTGCTTCACCATCCTGGGCGTGCTCACCAGCCTGGTGGTGCTGGGCACCGTGGCCATCGTCTTTCTCAAC AAGTGCGAAACCTGGGTGTCCAACCTGCGCTAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6581768), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58547. Forward Primer - name:058547_F_IRAV105_f08_Dlk1, sequence:GCATCTGCAAGGATGGCT; Reverse Primer - name:058547_R_SP6_IRAV105_f08_Dlk1, sequence:TGTAGCGCAGGTTGGAC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_012302 same experiment
	EMAGE:29847 same embryo
	EMAGE:30967 same embryo
	EMAGE:29848 same embryo
	EMAGE:29300 same embryo
	EMAGE:29855 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source