EMAGE mouse gene expression spatial database

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EMAGE:30999

Atp6ap1 ATPase, H+ transporting, lysosomal accessory protein 1 ( MGI:109629)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000573_01	euxassay_000573_02	euxassay_000573_03	euxassay_000573_04	euxassay_000573_05

euxassay_000573_06	euxassay_000573_07	euxassay_000573_08	euxassay_000573_09	euxassay_000573_10

euxassay_000573_11	euxassay_000573_12	euxassay_000573_13	euxassay_000573_14	euxassay_000573_15

euxassay_000573_16	euxassay_000573_17	euxassay_000573_18	euxassay_000573_19	euxassay_000573_20

euxassay_000573_21	euxassay_000573_22	euxassay_000573_23	euxassay_000573_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 01 weak expression: see section 06 07 08 09 10 14 15 16 17

facial vii ganglion

weak

homogeneous

weak expression: see section 17 18 19 23

vagus x ganglion

weak

homogeneous

weak expression: see section 15

trigeminal v ganglion

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 15 16 17 18 19 20 23

vestibulocochlear viii ganglion

weak

homogeneous

weak expression: see section 16 17

glossopharyngeal ix ganglion

weak

homogeneous

weak expression: see section 01 05 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2237
Entity Detected:	Atp6ap1, ATPase, H+ transporting, lysosomal accessory protein 1 ( MGI:109629)
Sequence:	sense strand is shown >T2237 GGCCTCNAGCCAGATTCGGATCCTTGATCACCAGCGATATGCAGCTTTCTACCTACTTAGACCCTGCCCT GGAGCTGGGTCCCCGTAATGTACTGCTGTTCCTACAGGACAAGCTAAGCATTGAGGATTTCACAGCATAC GGTGGTGTGTTTGGAAATAAGCAGGACAGTGCCTTTTCTAACCTGGAGAATGCCCTGGACTTGGCCCCCT CCTCACTGGTGCTTCCTGCTGTGGACTGGTATGCAATCAGCACTCTGACCACCTACCTACAGGAGAAGCT TGGGGCTAGCCCCTTGCATGTGGATCTAGCTACCTTAAAGGAGCTGAAGCTCAATGCCAGCCTTCCTGCC CTGCTGCTCATCCGTCTGCCCTACACAGCCAGCTCCGGTCTGATGGCGCCCAGGGAGGTCCTCACAGGTA ACGATGAGGTCATCGGACAGGTACTGAGCACACTCAAGTCTGAAGATGTCCCTTACACCGCAGCTCTTAC TGCAGTCCGCCCTTCTAGAGTGGCCCGTGATATAACCATGGTGGCTGGGGGTCTAGGTCGCCAGCTGCTT CAGACTCAGGTGGCA
Notes:	The probe template was PCR amplified from IMAGE:1050498 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1050498 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000573 same experiment
	EMAGE:30107 same embryo
	EMAGE:30415 same embryo
	EMAGE:30085 same embryo
	EMAGE:30187 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source