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EMAGE:31002

Mapt microtubule-associated protein tau ( MGI:97180)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31002 EMAGE:31002 EMAGE:31002 EMAGE:31002 EMAGE:31002
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
neural retina
strong strong
regionalstrong expression: see section 01 02 03
olfactory cortex marginal layer
strong strong
regionalstrong expression: see section 10 11 12 15 16 17
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 05 06 07 17 18
stomach
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11
kidney calyx
moderate moderate
regionalmoderate expression: see section 08 09 19 20 weak expression: see section 18
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 09 10 16
spinal cord
weak weak
homogeneousweak expression: see section 08 09 10 11 12 13 14 15 16 17
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 05 06
thoracic ganglion
strong strong
regionalstrong expression: see section 13 14 15
midgut
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 weak expression: see section 13 14 15 16 17 18 19 20
dorsal root ganglion
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 16 17
facial vii ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 18 19 20 21
not examined not examined
regionalnot examined expression: see section 01 02 03
cerebral cortex marginal layer
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21
midbrain
weak weak
homogeneousweak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
vagus x ganglion
strong strong
regionalstrong expression: see section 07 17
diencephalon
weak weak
homogeneousweak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
trigeminal v ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 17 18 19 20 21
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17
hindgut
moderate moderate
regionalmoderate expression: see section 12 weak expression: see section 13 14 15 16 17
telencephalon marginal layer
strong strong
regionalstrong expression: see section 02 moderate expression: see section 01
cervical ganglion
strong strong
regionalstrong expression: see section 08 16
telencephalon mantle layer
weak weak
regionalweak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
hindbrain
weak weak
homogeneousweak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 20
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4281
Entity Detected:Mapt, microtubule-associated protein tau ( MGI:97180)
Sequence:sense strand is shown

>T4281
CTCCAGCACCAGCAGCAGCGCCGCCGCCACCGCCCACCTTCTGCCGCCGCCGCCACAACCACCTTCTCCT
CCGCTGTCCTCTTCTGTCCTCGCCTTCTGTCGATTATCAGGCTTTGAACCAGTATGGCTGACCCTCGCCA
GGAGTTTGACACAATGGAAGACCATGCTGGAGATTACACTCTGCTCCAAGACCAAGAAGGAGACATGGAC
CATGGCTTAAAAGCCGAAGAAGCAGGCATCGGAGACACCCCGAACCAGGAGGACCAAGCCGCTGGGCATG
TGACTCAAGCTCGTGTGGCCAGCAAAGACAGGACAGGAAATGACGAGAAGAAAGCCAAGGGCGCTGATGG
CAAAACCGGGGCGAAGATCGCCACACCTCGGGGAGCAGCCTCTCCGGCCCAGAAGGGCACGTCCAACGCC
ACCAGGATCCCGGCCAAGACCACGCCCAGCCCTAAGACTCCTCCAGGGTCAGGTGAACCACCAAAATCCG
GAGAACGAAGCGGCTACAGCAGCCCCGGCTCTCCCGGAACGCCTGGCAGTCGCTCGCGCACCCCATCCCT
ACCAACACCGCCCACCCGGGAGCCCAAGAAGGTGGCAGTGGTCCGCACTCCCCCTAAGTCACCATCAGCT
AGTAAGAGCCGCCTGCAGACTGCCCCTGTGCCCATGCCAGACCTAAAGAATGTAGGGTCGAAGATTGGCT
CTACTGAGAACCTGAAGCACCAGCCAGGAGGTGGCAAGGTGCAGATAATTAATAAGAAGCTGGATCTTAG
CAACGTCCAGTCCAAGTGTGGCTCGAAGGATAATATCAAACACGTCCCGGGTGGAGGCAGTGTGCAAATA
GTCTACAAGCCGGTGGACCTGAGCAAAGTGACCTCCAAGTGTGGCTCGTTAGGGAACATCCATCACAAGC
CAGGAGGTGGCCAGGTGGAAGTAAAATCAGAGAAGCTGGACTTCAAGGACAGAGTCCAGTCGAAGATTGG
CTCCTTGGATAATATCACCCACGTCCCTGGAGGAGGGAATAAGAAGATTGAAACCCACAAGCTGACCTTC
AGGGAGAATGCCAAAGCCAAGACAGACCATGGAGCAGAAATTGTGTATAAGTCACCCGTGGTGTCTGGGG
ACACATCTCCACGGCACCTCAGCAATGTGTCTTCCACGGGCAGCATCGACATGGTGGACTCACCACAGCT
TGCCACACTAGCCGATGAAGTGTCTGCTTCCTTGGCCAAGCAGGGTTTGTGATCAGGCTCCCAGGGCAGT
CAATAATCATGGAGAGAAGAGAGAGTGAGAGTGTGGAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4504850 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4504850 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002990 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS